
The use of psychedelic substances like magic mushrooms is becoming more widely discussed, and with it comes a curiosity about whether these substances show up on drug tests, especially in the context of employment. Magic mushrooms, or shrooms, contain the psychoactive compounds psilocybin and psilocin, which produce profound changes in perception, mood, and consciousness. While standard drug tests do not typically screen for these compounds, specialized tests can detect them. The detection window for psilocin in urine is generally up to 24 hours after ingestion, but can extend up to 72 hours depending on factors such as dose and individual metabolism. Urine tests are the most common type of drug test and are preferred by many employers due to their convenience and low cost. Although rare, employers may test for psilocybin in certain cases, such as jobs with strict safety standards or a history of substance use.
| Characteristics | Values |
|---|---|
| Common drug tests for jobs | Do not test for shrooms |
| Detection in urine tests | Up to 24-72 hours after ingestion |
| Detection in blood tests | A few hours after ingestion |
| Detection in hair follicle tests | Up to 90 days |
| Metabolism | Rapid |
| Factors influencing detection time | Amount consumed, frequency of use, individual metabolism |
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What You'll Learn

Hallucinogen tests for mushrooms
Standard drug tests, such as the commonly used 5-panel urine test, typically screen for substances like THC, opioids, and amphetamines. Psilocybin and psilocin are not usually included in these routine tests, so mushrooms are unlikely to be detected unless a specific test for these compounds is requested.
Specialized hallucinogen tests for mushrooms do exist, but they are not in widespread use. These tests can detect psilocybin and psilocin in urine within a window of 1 to 3 days after consumption, although this window can be slightly longer for frequent users or those with slower metabolisms. Hair follicle testing can detect these compounds for up to 90 days, but these screenings are more expensive and less common than urine tests.
The duration of the effects of hallucinogenic mushrooms and the detectability of psilocybin and psilocin in the body depend on several factors, including dosage, species of mushroom, frequency of use, and individual metabolism. Higher doses and frequent use can lead to a longer detection window, as can a slower metabolism.
It is important to note that the legal status of psilocybin is complex and evolving. While it is classified as a Schedule I controlled substance under federal law in the United States and many other countries, indicating a high potential for abuse and no accepted medical use, some states, cities, and countries have decriminalized possession or are exploring its therapeutic potential through research and legislation.
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Standard drug tests and mushrooms
Standard drug tests typically do not test for psilocybin or psilocin, the active compounds in psychedelic or hallucinogenic mushrooms. These tests usually screen for more common substances, such as THC, opioids, amphetamines, cocaine, and phencyclidine (PCP). However, specialised tests can detect psilocybin and psilocin, although they are not widely used due to the higher cost and less frequent use of these substances.
The standard 5-panel or 10-panel urine tests, commonly used for employment screening, do not include psilocybin or psilocin. These tests focus on substances more commonly associated with workplace impairment and those with legal implications for safety-sensitive positions. However, certain job positions or legal circumstances may require a more extensive check, and employers can request specialised tests for psilocybin.
Specialised tests for psilocybin include urine, blood, saliva, hair follicle, and fingernail tests. Urine tests are the most common type, offering quick and reliable results. However, they have a shorter detection window, typically up to 24 hours after ingestion, which can extend to 72 hours depending on factors such as dose and individual metabolism. Blood tests are less common due to their invasive nature and even shorter detection window of only a few hours. Hair follicle and fingernail tests have longer detection windows, with hair tests providing a historical snapshot of drug use over approximately 90 days.
The accuracy of tests can vary, and false positives for psilocybin are uncommon but possible, especially with less specific tests due to potential cross-reactivity with other substances. The legal status of psilocybin also varies by area, with some states and cities decriminalising or legalising its use for potential therapeutic benefits. However, safety concerns remain due to the risks associated with psilocybin use, including panic attacks, psychotic episodes, and, in rare cases, death.
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Urine tests for mushrooms
Standard drug tests, such as the commonly used 5-panel urine test, typically screen for substances like THC, opioids, and amphetamines. Psilocybin and psilocin, the active compounds in hallucinogenic mushrooms, are not usually included in these routine tests, meaning that shrooms are unlikely to be detected unless a specific test for these substances is requested. Urine tests are the most common type of drug screening. While they do not routinely test for psilocybin or psilocin, specialized urine tests can detect these substances.
The detection window for psilocin in urine is generally up to 24 hours after ingestion, although it can extend to 72 hours depending on factors such as the dose taken, the individual’s metabolism, and how often the person uses mushrooms. The longer it takes the body to break down and excrete the compounds, the longer they are detectable in a urine test.
After ingesting hallucinogenic mushrooms, the body breaks them down into their active components, psilocybin and psilocin. These compounds act on the brain to produce changes in emotion, perception, and consciousness, which can start in as little as 20–30 minutes after ingestion. The kidneys are responsible for excreting these compounds, which then leave the body in the urine. The half-life of a drug is how long it takes the body to break down or excrete half of the total amount ingested.
To test for mushrooms, a technician will provide a cup in which to supply a urine sample. The urine is then tested by liquid chromatography/mass spectrometry (LC/MS-MS) at a threshold of 1.0 ng/mL. If the first test is positive, a second confirmatory test is run using the same method to ensure the most accurate result. Results from these tests typically take 7-14 business days, with an additional 2-4 business days to confirm a positive result.
It is important to note that hair follicle testing can detect psilocybin and psilocin for up to 90 days, but these screenings are less common due to their higher cost. Blood and saliva tests are even less common due to their invasive nature and short detection window, as psilocin is typically detectable in blood for only a few hours post-ingestion.
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Blood tests for mushrooms
Psilocybin mushrooms, also known as magic mushrooms or shrooms, are hallucinogenic and contain the psychoactive compound psilocybin. This compound is converted into psilocin in the body, which is responsible for the drug's hallucinogenic effects. Shrooms are not typically included in standard drug tests, which often screen for substances like THC, opioids, and amphetamines. However, they may show up on specific tests designed to detect hallucinogens, although these are not commonly used.
Blood tests for psilocybin are rarely ordered unless there is a specific reason to do so. Psilocybin and psilocin are typically detectable in the blood for only a few hours after ingestion, so blood tests are most useful when recent use is suspected. The detection window for psilocin in urine is generally up to 24 hours after ingestion but can extend to 72 hours depending on factors such as the dose and the individual's metabolism.
Hair follicle testing can detect psilocybin and psilocin for up to 90 days, but these tests are less common due to their higher cost. Standard urine tests are the most common type of drug screening and are typically a five-panel test that does not include psilocybin or psilocin. However, specialized urine tests can detect these compounds.
The body metabolizes psilocybin and psilocin relatively quickly, and most people can expect the compounds to be out of their system within a day. The speed at which the body processes these compounds depends on various factors, including sleep, hydration, diet, chronic diseases, genetics, weight, and age.
While shrooms are not routinely tested for in workplace settings, specialized tests can detect their presence. If you are concerned about your substance use or the effects of shroom use, professional support and treatment programs are available.
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Hair follicle testing for mushrooms
Standard drug tests, such as the commonly used 5-panel urine test, typically screen for substances like THC, opioids, and amphetamines. Psilocybin and psilocin, the active compounds in magic mushrooms, are not usually included in these routine tests, meaning that shrooms are unlikely to be detected unless a specific test for these substances is requested.
Hair testing can be performed on hair from 30 days to up to a year, depending on the hair, the timeframe selected, or the location where the specimen was collected. Higher levels of a substance are a good indication of use history but could also be from one-time use of a lot. Dark hair retains drugs more easily, and UV light and hair treatments only seem to affect THC or other acidic drugs.
While hair follicle testing for mushrooms is possible, it is not as common as urine tests. Federally regulated programs currently only collect and test urine.
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Frequently asked questions
Most standard drug tests don't screen for psilocybin, the active ingredient in "magic mushrooms". However, there are rare cases where employers can test for psilocybin, especially if a job involves strict safety standards or if there's a history of substance use.
Urine tests are the most common type of drug test and can detect psilocybin within 24 hours of use. Blood tests are less common due to their invasive nature and short detection window. Hair follicle testing can detect psilocybin for up to 90 days, but it is more costly and less common.
Psilocybin is rapidly metabolized by the body, with most of the compound being eliminated within 24 hours of consumption. However, the detection window can vary depending on factors such as the dose, frequency of use, and individual metabolism.
No, there are no known legal medications that can cause a false positive for psilocybin or psilocin on a drug test.
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