
Mushrooms are the reproductive bodies of fungi, and their purpose is to produce spores or seeds. Each type of fungus has a unique spore type and releases them in distinct patterns depending on the form of the underside of the mushroom cap. Gill mushrooms are the easiest to harvest spores from, but all types can be harvested with some experimentation. The most common method for harvesting spores from mushrooms is making a spore print, which is used to identify a fungus. Spore prints can be made by placing the mushroom cap spore side down onto a piece of paper and covering it with a glass container to prevent it from drying out. The spores will drop onto the paper within a few hours or overnight. These spores can then be used to grow mushrooms. While it is possible to grow mushrooms from old spores, there are mixed reports on the success of this method, with some reporting slower growth and more mutants.
| Characteristics | Values |
|---|---|
| Freshness | Fresh mushrooms don't keep for very long and have a much shorter shelf life compared to dried ones. Fresh mushrooms can spoil within a few days if not properly refrigerated, while dried and stored mushrooms can last for months or even years. |
| Spoilage signs | Bad mushrooms look and feel slimy, have brown spots or discoloration, smell fishy or sour, or have mold. |
| Storage | Mushrooms should be kept in a cool, dark, and dry environment. For fresh mushrooms, refrigeration slows down decomposition, while dried mushrooms should be stored in airtight containers to minimize exposure to air and moisture. |
| Consumption | Old mushrooms can be consumed, but they may not look as pretty as fresh ones. They can be cooked in olive oil with garlic and herbs and served with pasta, as a side dish, or with fish or chicken. |
| Potency | Aged mushrooms may result in unintentional trips due to irregular potency. The main psychoactive compound, psilocybin, decomposes over time, and factors like light, heat, and air speed up this process. |
| Preservation | Mushrooms can be preserved in honey for up to a year, but the sugar content and type of mushroom should be considered. |
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What You'll Learn

Old spores can work, but they may produce more mutants
Mushroom cultivation is a complex process that requires specific equipment and techniques. While it is true that certain conditions, such as temperature and humidity, can encourage mushroom growth, cultivating mushrooms from spores is intricate. Old mushroom spores can work, but they may produce more mutants and exhibit slower growth rates.
One individual reported that they were able to germinate old mushroom spores stored in water. However, they observed that the growth post-germination was not as robust as that of fresh spores, and they also noticed an increase in mutant spores. This finding aligns with another user's experience, who described their old spores as "exceedingly slow growers" that generated a significant number of aborts.
The viability of old mushroom spores may be enhanced through sexual reproduction. After fruiting, the resulting spores may exhibit increased vigour. However, it is unclear whether old spores can be invigorated before fresh reproduction. Some individuals have expressed skepticism about the idea that old spores can become 100% healthy after being dormant for an extended period.
The success of cultivating mushrooms from old spores may depend on various factors, including the specific mushroom species and the environmental conditions. It is recommended to start with a small batch when trying a new species to understand its unique requirements without committing extensive resources. Additionally, maintaining a cultivation journal can be helpful for recording growth conditions, stages, and any challenges encountered during the process.
Overall, while old mushroom spores can work, they may not produce the same vigorous growth as fresh spores and may lead to a higher proportion of mutants. For optimal results, it is advisable to collect fresh spores and follow the recommended cultivation techniques for the specific mushroom species.
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Old spores can be reinvigorated through sexual reproduction
Fungi, including mushrooms, reproduce both asexually and sexually. Asexual reproduction involves the production of spores by one parent only, and these spores are genetically identical to the parent. On the other hand, sexual reproduction involves two parents, resulting in offspring with a mix of genes from each parent. This genetic diversity is advantageous as it helps the species survive by enabling different individuals to respond uniquely to threats and challenges.
Sexual reproduction in fungi is often triggered by adverse environmental conditions. It allows the fungus to adapt to new environments. Fungi employ various methods to bring together two compatible haploid nuclei. Some produce specialized sex cells (gametes) released from differentiated sex organs, while others have somatic (vegetative) hyphae that take over the sexual function, fusing and exchanging nuclei.
Mushrooms, being a type of fungus, also reproduce sexually. They produce spores that carry genetic information from both parents. These spores are released and dispersed through convectively created airflows, even in low-wind environments. While mushrooms can produce trillions of spores, the probability of any single spore establishing a new individual is very small.
While the viability of old mushroom spores over time is unclear, it is known that spores can last about a year when stored in favorable conditions. Therefore, it is plausible that old mushroom spores could be reinvigorated through sexual reproduction, assuming they retain some viability. By fusing with fresh spores or compatible nuclei, old spores could potentially regain vitality and contribute to the formation of new, genetically diverse offspring.
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Old spores may be less vigorous than fresh spores
While mushroom spores can last about a year if stored in favourable conditions, old spores may be less vigorous than fresh spores. One source suggests that old spores, once reproduced, will be vigorous. However, this same source also suggests that the best isolate from fresh spores is likely to be a better performer than the best isolate from old spores.
Indeed, one user on a mushroom cultivation forum noted that their old spores, which were stored in water, were able to germinate, but the growth post-germination was less impressive than that of fresh spores, and there were more mutants. Another user agreed that sexual reproduction would invigorate old spores, but they were unsure whether to expect less vigour before fresh reproduction.
Another user on the same forum noted that their old spores were exceedingly slow growers and generated a lot of aborts. However, another user replied that they had seen a 20-year-old spore print that was fine, suggesting that the performance of old spores depends on various factors.
In addition to the age of the spores, the success of mushroom cultivation depends on factors such as temperature, humidity, and the growing substrate. For instance, coffee grounds are a poor substrate for growing Agaricus mushrooms, as they are very prone to contamination.
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Old spores can be stored in water
Old mushroom spores can be stored in water, but it is important to note that the spores should be placed in a sterile water solution. The use of sterilized water is crucial to prevent contamination and ensure the longevity of the spores. Here are some detailed guidelines for storing mushroom spores in water:
Sterilizing the Water
To sterilize the water for storing mushroom spores, you have two options. One method is to boil the water, let it cool down, and then boil it again. Alternatively, you can pressure cook the water in a beaker at 15 psi for 15 minutes. This process ensures that the water is free from any contaminants that could harm the spores.
Preparing the Spore Syringe
Mushroom spores are typically stored in syringes, which are then placed in sealed bags and stored in the refrigerator. To prepare the spore syringe, you will need to mix the spores with the sterilized water. This mixture is then drawn into the syringe, creating a spore-water solution. It is important to ensure that the syringe is also sterilized before use to maintain a sterile environment for the spores.
Storing the Spore Syringes
The spore syringes should be stored in a sealed, sterile environment to prevent contamination. Store the syringes in a sealed container or bag, and always use clean hands or gloves when handling them. It is recommended to store the spore syringes in the refrigerator at temperatures between 2-8°C (35-46°F). This temperature range will keep the spores dormant but not frozen, preserving their viability.
Longevity and Viability
Properly stored mushroom spores can last for a long time, typically remaining viable for up to two years or more. However, for optimal results, it is recommended to use the spores within 12-18 months. Refrigeration helps slow down the metabolic processes of the spores, allowing them to remain viable for extended periods.
In summary, old mushroom spores can be successfully stored in water by creating a sterile spore-water solution and following careful storage procedures. By following these guidelines, you can prolong the viability of your mushroom spores and increase their longevity.
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Old spores may be more prone to contamination
While there is limited information on the performance of old mushroom spores, some sources suggest that they can still be used to grow mushrooms. However, the growth and post-germination of old spores may not match that of fresh spores, with one source noting that their old spores produced more mutants.
Additionally, old spores may benefit from sexual reproduction, as the resulting spores will be more vigorous. However, it is unclear whether old spores will be less vigorous before fresh reproduction. Some sources suggest that old spores, even if half-decayed and barely viable, become 100% healthy once they "wake up."
Overall, while old mushroom spores may still be usable, they may not perform as well as fresh spores and could be more susceptible to contamination during the longer growth process.
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Frequently asked questions
Old mushroom spores can work, but they may not be as effective as fresh spores. Old spores may result in slower growth and more mutants.
If you live in a humid area, it is recommended to store old mushroom spores in the fridge. Do not freeze the spores. Kept in favourable conditions, spores can last about a year.
Old mushroom spores can be used in the same way as fresh spores. To grow mushrooms from spores, you will need a mushroom growing kit, a suitable substrate, and sometimes a spore syringe to inoculate the growing medium.

























