
Carbon dioxide (CO2) is an important factor in mushroom cultivation, affecting growth and yield. While mushrooms do not require CO2 for photosynthesis, it is crucial for the development of their fruiting bodies and the growth of mycelium. Optimal CO2 levels vary depending on the mushroom species and growth stage, but generally, too much or too little can be detrimental to mushroom growth. Therefore, monitoring and controlling CO2 levels in the growing environment is essential for successful mushroom cultivation, including that of psilocybin mushrooms.
| Characteristics | Values |
|---|---|
| Do psilocybin mushrooms need CO2? | Unlike plants, mushrooms do not need CO2 for photosynthesis. They get all the CO2 they need from the air around them. However, CO2 is critical for the growth of the mycelium and the development of the fruiting body. CO2 levels must be maintained at the right level to achieve optimal growth and yield. |
| CO2 levels for psilocybin mushrooms | Most experts suggest CO2 levels between 10,000-20,000 ppm during the spawn run, and no more than 1,000 ppm during the fruiting phase, preferably between 300-800 ppm. |
| Methods for controlling CO2 levels | Ventilation, air exchange, and the use of a CO2 controller or sensor. |
| Tools for measuring CO2 levels | A CO2 meter is a small device that can help monitor and control CO2 levels in the growing environment. |
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What You'll Learn

CO2 is critical for the growth of the fruiting body
Carbon dioxide is an important factor in mushroom farming. It is critical for the growth of the fruiting body and for the mycelium to grow. Mushrooms require oxygen to grow, and during respiration, they release CO2, just like humans. Therefore, the correct CO2 level is crucial for successful mushroom farming.
CO2 levels that are too high can cause the mushrooms to grow too quickly, resulting in poor quality and low yield. On the other hand, insufficient CO2 can slow down mushroom growth and result in small or deformed mushrooms. The appropriate CO2 concentration can stimulate the differentiation of fruiting bodies, and excessive concentrations can inhibit the growth of the hypha. In the fruiting body stage, edible fungi are more sensitive to CO2 concentration.
To achieve optimal growth and yield, CO2 levels must be carefully controlled. Most experts recommend CO2 levels between 10,000 and 20,000 ppm during the spawn run but no more than 1,000 ppm during the fruiting phase, ideally between 500 and 800 ppm. If CO2 levels exceed 1,000 ppm during fruiting, yields will decrease. Additionally, carbon dioxide monitoring is crucial during pinning, a critical phase in the mushroom's growth cycle.
To maintain the proper CO2 levels, mushroom cultivators can employ CO2 generators and CO2 meters. CO2 meters are portable devices that allow users to monitor and adjust CO2 levels in their mushroom-growing environment. They are essential tools for optimising mushroom growth and ensuring high-quality, large mushrooms.
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CO2 levels must be monitored and controlled
Carbon dioxide (CO2) is an essential gas for mushroom growth, but it must be maintained at the right level to achieve optimal growth and yield. CO2 is critical for the development of the fruiting body and for the mycelium to grow. Mushrooms require oxygen and release CO2 during respiration. Therefore, the correct CO2 level is needed for successful mushroom farming.
CO2 levels can be controlled through ventilation, air exchange, and the use of a CO2 controller or sensor. A CO2 meter is a small but powerful device that can help monitor and control CO2 levels in your mushroom-growing environment. It measures the CO2 level and adjusts the ventilation or air exchange system to maintain the desired CO2 level. With a CO2 meter, you can easily detect when CO2 levels are too high or too low, and make adjustments accordingly.
To measure the CO2 level in your mushroom-growing environment, a CO2 sensor is used. A CO2 sensor measures the CO2 level in the air and displays the reading in parts per million (ppm). It can be used to monitor CO2 levels in real time and allows you to make adjustments accordingly. Most experts suggest CO2 levels between 10,000-20,000 ppm during the spawn run but no more than 1,000 ppm during the fruiting phase, preferably between 500 and 800 ppm.
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Mushrooms require fresh air
The process of mushroom growth involves releasing carbon dioxide (CO2) and consuming oxygen (O2). Mushrooms, like humans, require oxygen for respiration and produce carbon dioxide as a by-product. When cultivating mushrooms in an enclosed space without sufficient air exchange, oxygen depletion can occur, impeding growth and even suffocating the mycelium. Therefore, it is crucial to ensure proper ventilation and fresh air circulation to maintain optimal oxygen levels for the mushrooms' growth.
Effective air exchange helps regulate humidity levels, ensuring that the growing medium remains appropriately moist. Fans play a vital role in achieving the necessary air exchange by providing a continuous supply of fresh oxygen. They also aid in carbon dioxide ventilation, helping to maintain lower CO2 levels, which are vital for the development of healthy mushrooms. Additionally, air circulation generated by fans deters the accumulation of contaminants, reducing the risk of mold, bacteria, or other harmful microorganisms.
The amount of fresh air required for an optimum growing environment varies depending on the type of mushroom. For example, mushrooms from the Panaeolus family require more frequent air refreshment than those from the Psilocybe family. It is recommended to refresh the air a minimum of 3 to 4 times per day for Psilocybe mushrooms and 6 to 8 times per day for Panaeolus mushrooms.
To maximize the benefits of fans in a mushroom-growing setup, strategic placement and appropriate fan size are crucial. It is also beneficial to use a timer or controller to regulate the fan's operation, allowing for intermittent air exchange. Regular maintenance of the fans is essential to keep them clean and maintain their efficiency. Overall, ensuring proper fresh air exchange is pivotal to successful mushroom cultivation.
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CO2 is essential for mushroom respiration
Carbon dioxide is an important factor in mushroom farming because it directly affects the growth and yield of mushrooms. Mushrooms require oxygen for respiration, and during this process, they release carbon dioxide, similar to humans.
CO2 is essential for the development of the mushroom's fruiting body and for the mycelium to grow. The mycelium is a branching, thread-like colony of fungus that grows in compost, which is why mushrooms are often seen growing out of dead logs or tree branches on forest floors. When the mycelium is ready, growers "shock" the fungus by lowering the temperature, reducing the CO2 level, and increasing evaporation.
CO2 levels need to be carefully monitored and controlled in mushroom growing environments. While mushrooms do not require CO2 for photosynthesis, as they are not plants, too much CO2 can cause mushrooms to grow too quickly, resulting in poor quality and low yield. Conversely, too little CO2 can slow down growth and result in small or deformed mushrooms. Therefore, it is crucial to maintain optimal CO2 levels, usually between 10,000 and 20,000 ppm during the spawn run and no more than 1,000 ppm during the fruiting phase, preferably between 300 and 800 ppm.
To control CO2 levels, growers can employ strategies such as ventilation, air exchange, and the use of CO2 controllers or sensors. Regular fans can be used to bring in fresh air, and more specialized equipment, such as CO2 meters and controllers, can help monitor and adjust CO2 levels to ensure optimal conditions for mushroom growth.
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CO2 levels affect mushroom morphology
CO2 levels are critical to the success of mushroom farming, as they influence the growth, morphology, and overall yield of mushrooms. Mushrooms require oxygen for growth, and high CO2 levels can cause issues with mushroom morphology, resulting in deformed or stunted mushrooms with long stems as the developing fruit pushes higher in the air to search for oxygen. This phenomenon is known as "legging".
CO2 is critical for the development of the fruiting body and for the mycelium to grow. Mycelium is the vegetative part of the mushroom and requires specific conditions to thrive, including consistent temperature, humidity, and airflow. During the spawn run, most experts suggest CO2 levels between 10,000–20,000 ppm, with high levels of up to 10,000 ppm being beneficial for mycelial growth. However, during the fruiting phase, CO2 levels should be reduced to no more than 1,000 ppm, preferably between 500 and 800 ppm. If CO2 concentrations are higher than 1,000 ppm during fruiting, yields will decrease, and the mushrooms may develop long, skinny stems and small caps, which are less desirable in the market.
To maintain optimal CO2 levels, mushroom farmers employ various techniques such as ventilation, air exchange, and the use of CO2 controllers or sensors. Exhaust fans are commonly used to reduce CO2 levels, while humidifiers help maintain the high humidity levels that mushrooms require. Additionally, the choice of substrate can influence CO2 levels, as some substrates release more CO2 during decomposition.
By managing CO2 levels effectively, mushroom farmers can create an ideal environment for their crops, maximizing yield and ensuring the highest quality mushrooms for the market.
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Frequently asked questions
Psilocybin mushrooms do not require additional CO2 as they get all the CO2 they need from the air around them. However, CO2 levels must be monitored and controlled to ensure optimal growth and yield.
The optimal CO2 levels for growing psilocybin mushrooms vary depending on the growth stage. During colonization, CO2 levels can range from 5000 to 10,000 ppm, while during fruiting, levels should be reduced to around 300 ppm.
CO2 levels can be controlled through ventilation, air exchange, and the use of CO2 controllers or sensors. Regular fans or intake and exhaust fans can be used to bring in fresh air and maintain CO2 levels below 1000 ppm for most psilocybin mushroom species.

























