
Mushroom grow bags are an essential tool for cultivating mushrooms, used by both professionals and hobbyists. They can be used as spawn bags for sterilizing grain and propagating mushroom cultures or for creating supplemented sawdust fruiting blocks for gourmet mushrooms. The process of growing mushrooms in bags involves preparing the substrate or growing medium, which can include grain, straw, or wood chips. The substrate is then sterilized and placed inside the bag, after which mushroom spawn is added to inoculate the bag. The bag is then sealed and placed in a suitable environment for the mycelium to colonize the substrate. While some growers choose to fruit their mushrooms within the bag, others opt for alternative fruiting chambers. Proper harvesting techniques are crucial to ensure the integrity of future flushes. Additionally, the ability to reuse grow bags for various purposes adds to their appeal.
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What You'll Learn

Preparing the grain spawn
Selecting the Grain
The first step is to choose the right grain for your mushroom spawn. Various grains can be used, including wheat, rye, brown rice, white rice, wild bird seed, and even popcorn. Among these options, rye is considered ideal due to its water-absorbing qualities, nutrient content, and ability to support mushroom growth. However, rye may be challenging to source and expensive for small-scale growers. In such cases, wheat, which is the most commonly used grain in the UK, can be a suitable alternative. Millet is another option that provides smaller grains, resulting in more inoculation points when added to a bulk substrate.
Soaking the Grain
Once you have selected your grain, it's time to soak it. Place the grain in a bucket or bowl, cover it with water, and let it soak for 12 to 24 hours. This process ensures the grains are adequately hydrated, making them softer and easier for the mushroom mycelium to consume.
Simmering the Grains
After soaking, the grains need to be simmered on the stove. This step further hydrates the grains and makes them even softer. Simmer the soaked grains for about 15 minutes on low heat. Be careful not to overcook them, as prolonged cooking can lead to mushy grain spawn.
Draining and Filling Jars
Once the grains have been simmered, it's time to drain them. Simply place them in a sink with a drain cover and let them drain for about an hour. After draining, fill your jars about two-thirds to three-quarters full. Filling the jars to this level allows for easier shaking during the colonization process, speeding up mushroom growth.
Sterilization
Cover the jar lids with tinfoil to prevent water from entering during sterilization and altering the moisture content. Using a pressure cooker or autoclave, sterilize the jars at 15 PSI for at least 90 minutes. This step is crucial for killing any contaminants in the grain that could compete with your mushroom culture. Ensure you use a good-quality pressure canner that can reach and maintain 15 PSI.
Cooling and Inoculation
After sterilization, turn off the stove and let the jars cool for several hours before handling. Once cooled, your jars are ready for inoculation with mushroom culture. You can use a spore syringe or liquid culture to introduce the mushroom mycelium into the sterilized grain.
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Sterilizing the grain
The first step in the process of sterilizing grains for mushroom cultivation is preparing the grains. Proper preparation ensures that the grains absorb the right amount of moisture without becoming too wet, which can lead to contamination. The quantity of grains needed depends on the size of the grow bags. A general rule is about 1-2 cups of dry grain per bag.
Once the grains are measured out, they should be soaked in water. After soaking, drain the water and transfer the grains to a pot. Cover the grains with fresh water and bring them to a boil for 10-15 minutes. This ensures the grains are fully hydrated without becoming mushy.
Next, use a strainer to drain the boiled grains. Spread the grains out on a clean surface or towel to air dry for about 30 minutes. Proper drying prevents excess moisture inside the grow bags.
Now the grains are ready to be loaded into mushroom grow bags. Fill each bag halfway to allow space for mycelium growth and gas exchange. Fold the top of the bags but do not seal them completely to prevent pressure build-up during sterilization.
To protect the filter patches on the grow bags from getting wet during sterilization, cover the top of each bag with aluminum foil. The bags are now ready for the sterilization process.
It is important to note that sterilization ensures that only the desired mushroom spores or mycelium can colonize the grains, preventing competition from contaminants. The sterilization process for grains in mushroom grow bags typically involves the use of a pressure cooker. The pressure cooker should be capable of reaching 15 PSI, and the grains should be sterilized for a duration of 90 minutes to ensure all endospores and contaminants are destroyed.
After sterilization, turn off the heat and allow the pressure cooker to cool naturally. Do not attempt to open it until the pressure gauge reads zero to avoid accidents. Once the pressure cooker has cooled, carefully remove the grow bags, wearing heat-resistant gloves to handle the hot bags.
Avoid touching the inside of the bags to maintain sterility. Allow the bags to cool completely before inoculating them with mushroom spores or mycelium. This step should be done in a clean environment to prevent contamination. Sanitize the workspace using alcohol or a disinfectant.
Finally, inject the spores or mycelium into the grow bag through the self-healing injection port or seal using a sterilized syringe.
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Inoculating the bag
Prepare the Grain Spawn
Start by selecting an appropriate grain such as rye, wheat, milo, millet, brown rice, white rice, or even wild bird seed. Rinse and clean the grains thoroughly to remove any dust, wood chips, or other contaminants. Then, soak the grains in water for 12 to 24 hours to initiate the hydration process. This step is crucial as it prepares the grains to better absorb water and facilitates mycelium growth once they are inoculated.
Boil and Drain the Grains
After soaking, drain the grains and briefly boil them for 10 to 20 minutes until they are fully hydrated but not mushy. This step ensures that the grains are properly hydrated and ready for the next stage. Drain and rinse the boiled grains to remove any excess water and starch. It is important to ensure that the grains are not overly moist, as this can affect the inoculation process.
Dry and Load the Grains
Spread the drained grains into a thin layer and allow them to dry for about an hour or two. The grains should have no noticeable moisture on the outside. Once dry, load the grains into your chosen containers, such as mason jars or grain bags. Fill the containers to about two-thirds or three-quarters full, leaving some space for the grains to expand during sterilisation.
Sterilise the Grain
Before inoculation, it is essential to sterilise the grains to eliminate any potential contaminants. You can use an autoclave, a pressure cooker, or a pressure steriliser. For mason jars, sterilise them for 90 minutes at 15 PSI. If using pre-sterilised grain bags, ensure they are stored at room temperature out of direct sunlight, and they can be stored for several months before use.
Inoculate the Bag
Now you are ready to inoculate the grow bag. Wear clean gloves to reduce the risk of introducing contaminants. Using a spore syringe or liquid culture, inject the spores or culture through the injection port of the grow bag. Ensure you follow the recommended dosage, typically 2.5-5ccs per 3-pound injection port bag. Avoid using an entire syringe in one bag, as it can create excessive moisture and oversaturation.
Seal the Bag
After inoculation, seal the mushroom grow bag using an impulse sealer to ensure an airtight seal, which helps prevent contamination. If you don't have an impulse sealer, you can carefully fold the top of the bag over a few times and secure it with paper clips or binder clips. Ensure the bag stands upright to facilitate proper gas exchange through the filter patches.
Initiate Fruiting
Once your bag is colonised, you can initiate fruiting. For beginners, it is recommended to fruit within the bag. Make a slice or hole in the bag just above the substrate to allow for fresh air exchange. Maintain optimal environmental conditions, including fresh air, humidity, light, and temperature, to trigger fruiting from fully colonised substrates.
Remember to refer to specific guides and instructions for your chosen mushroom species, as each variety may have unique requirements for optimal growth. Happy mushroom cultivating!
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Initiating fruiting conditions
Fresh air exchange is crucial for mushroom growth. The ShroomTek bag system, for example, is designed to achieve optimal fresh air exchange while allowing the cultivator control over light and temperature. A 5-micron filter or 5-micron filter patch is better suited for fruiting as it allows more fresh air exchange.
Mushrooms do not use light as an energy source to grow, but they need some light to grow properly. Indirect light from a window or artificial lighting can be used. If using artificial lighting, it is recommended to keep it on for 12 hours a day, and a digital timer can help automate this process.
Some growers cut holes in the bag to allow mushrooms to grow out from various directions. For example, the Reishi mushroom will grow long and twisted "antlers" in the high CO2 environment inside the bag, and if left long enough, it will fruit right through the filter.
Once the mushrooms are ready, simply open the bag and harvest your bounty.
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Harvesting the mushrooms
Harvesting mushrooms grown in bags requires a careful technique to avoid damaging the mycelium and reducing future yields. To harvest the mushrooms, gently grasp the mushroom at its base and twist it slightly to remove it cleanly. Avoid yanking or pulling the mushrooms out, as this could damage the mycelium and affect future growth.
After the first harvest, you can initiate additional flushes by rehydrating the substrate using a dunking process. Fill the bag with cold water, making sure the substrate is fully submerged, and let it soak for 12 to 24 hours. Then, drain the water from the bag. This process replenishes the moisture content and prepares the bag for another flush.
To promote the next flush, place the bag in a dark, conducive environment for mycelium recovery after the dunking process. Leave the bag undisturbed for a few days to allow the mycelium to recover and initiate the next flush.
Some mushrooms, like the Reishi mushroom, may grow inside the bag in unique ways. In the high CO2 environment of the bag, Reishi mushrooms may develop long and twisted "antlers" instead of their typical shape. If left to grow, they will eventually fruit right through the filter.
When harvesting mushrooms from grain bags, it is important to use a gentle technique and ensure proper rehydration practices to maintain the health of the mycelium and maximize your yield.
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Frequently asked questions
Mushroom grow bags are an essential tool for cultivating mushrooms. They can be used as spawn bags for sterilizing grain and propagating mushroom cultures, or to create supplemented sawdust fruiting blocks for growing gourmet mushrooms.
First, select a suitable grain such as rye, wheat, milo, or millet. Then, soak the grain in water for 12-24 hours to partially hydrate it. Larger or harder grains may need to be boiled for 10-20 minutes. After soaking or boiling, drain and rinse the grain to remove excess water and starch. Finally, transfer the hydrated grain into a mushroom grow bag.
First, open the top of your bag in a sterile working environment. Then, pour your mushroom grain spawn into the bag, distributing it evenly throughout the substrate material inside. Gently massage and mix the bag to integrate the spawn without compacting the substrate. Finally, securely re-seal the opened top of your bag to protect the spawn from contamination.
Expose the bag to fresh air and light to encourage mushroom fruiting. Some growers cut holes in the bag to allow mushrooms to grow out from various directions.
Gently grasp the mushroom at the base and give it a slight twist to remove it cleanly. Avoid yanking or pulling as it could damage the mycelium. After the first harvest, you can initiate subsequent flushes by rehydrating the substrate through a dunking process.

























