
The Glowing Mushroom Biome in Terraria is a treacherous yet rewarding landscape. With giant glowing mushrooms and unique monsters, it is a challenging biome to traverse. Players can create their own glowing mushroom biome by locating a natural biome and harvesting mushroom grass seeds from the mushrooms. These seeds can then be planted in mud, and will spread to adjacent mud blocks. Players should be warned, however, that creating this biome on the surface will spawn hard mode enemies, even if the player is not in hard mode.
| Characteristics | Values |
|---|---|
| Location | Above or below sea level |
| Requirements | Mushroom grass seeds, mud, water source |
| Number of blocks | 100 |
| Placement | Blocks should be placed opposite each other |
| Waiting time | 5-6 in-game days |
| Music | Music will change once the biome is ready |
| Background | The background will become darker |
| Housing | A house must be built for the Truffle NPC to spawn |
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What You'll Learn

Find a natural mushroom biome and harvest seeds
Mushroom biomes are rare and can be challenging to find. They are often located in the middle of the ocean, surrounded by deep waters. To find a natural mushroom biome, you can follow these steps:
- Look for ocean-adjacent locations: Mushroom biomes are typically found near or touching large bodies of water. They are often islands or floating islands, so focus your search on areas with deep oceans and few nearby landmasses.
- Check coordinates: Mushroom biomes tend to have high X and/or Z coordinates. To view your coordinates in-game, use the key combination F3 or Shift+F3. A mushroom biome might have coordinates like 3000 x, 98 z, or 768 x, 3576 z. Most will have at least one coordinate exceeding 3000.
- Maintain a straight sailing path: When sailing to explore potential locations, it is recommended to go in straight or diagonal lines. Changing directions too often may cause you to miss a mushroom biome, as they can be elusive.
- Utilize world seeds: Certain world seeds are known to contain mushroom biomes. You can obtain these seeds, find their coordinates, and use that information to navigate to the desired location.
Once you have successfully located a natural mushroom biome, you can proceed to harvest the seeds to create your own glowing mushroom biome. Here are the steps to harvest mushroom seeds:
- Identify mature mushrooms: Look for mature mushrooms with fully developed caps. These mushrooms should be from a known edible variety to ensure safety.
- Remove the mushroom stem: Carefully detach the stem from the mushroom. Gently remove any skirt or protective layer covering the gills in the mushroom head. You will be working with the top portion of the mushroom that contains the gills.
- Prepare a sterile surface: Use a piece of paper or foil as a sterile surface. You will place the mushroom on this surface to collect the spores.
- Create a spore print: Place the mushroom with the gills facing down onto the paper or foil. Cover the mushroom with a glass or container to protect it from contamination. Leave it undisturbed for 24 hours.
- Harvest the spores: After 24 hours, carefully lift the mushroom. You will notice that the spores have fallen from the cap, creating a spore print on the paper or foil. This print replicates the gill pattern and contains the spores necessary for new mushroom growth.
- Propagate the spores: One mature mushroom can contain up to 16 billion spores, providing ample opportunities for propagation. You can use the spore print to inoculate a prepared substrate, such as compost or substrate blocks. Maintain humid and dark conditions to encourage successful mushroom growth.
By following these steps, you can locate a natural mushroom biome and harvest the seeds needed to create your own glowing mushroom biome. Remember to be patient and meticulous during the process, as growing mushrooms from spores requires dedication and attention to detail.
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Convert dirt into mud
To convert dirt into mud, you can use a water bottle on a dirt block. This process can be automated using a dispenser. Another way is to craft buckets, stand in water, fill the bucket, and then use the water to craft mud.
Mud is an essential component of creating a glowing mushroom biome. To build a glowing mushroom biome, you will need to find a natural glowing mushroom biome and harvest the glowing mushrooms to obtain mushroom grass seeds. Alternatively, you can chop down the mushrooms, and at least one should give you a mushroom grass seed. Once you have the seeds, head to a source of water and convert dirt into mud as described above. Mine a hole where you want your glowing mushroom biome, fill it with mud (around 250-300 blocks should be enough), and plant your mushroom grass seeds. You will need about 100 blocks of blue grass in total, so be sure to put the seeds on opposite sections to speed up the spread. As the mushroom grass starts to grow, the background will gradually get darker, and the music will change when the biome is officially formed.
If you are playing the game Terraria, it is important to note that placing glowing mushrooms will not count towards creating the biome. However, the glowing mushroom grass will spread to adjacent mud blocks. One effective strategy is to make a 1x100 mud strip in the sky and plant 4 mushroom grass seeds in random places on it. After about 5-6 in-game days, the whole strip of mud should be covered in mushroom grass.
Additionally, building a house in the biome is necessary for the Truffle NPC to spawn. You can use glowing mushrooms to make the house appear as though it was constructed from the same material as the biome.
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Plant seeds in mud
To create a glowing mushroom biome, you will need to plant mushroom grass seeds in mud. Here is a step-by-step guide on how to do this:
First, find a natural glowing mushroom biome and harvest the glowing mushrooms to obtain mushroom grass seeds. You can also get dirt and make your own mud. Chop down the mushrooms and collect the dirt as you go. You will need around 250-300 blocks of mud for your biome.
Next, find a source of water and stand within about two blocks of it. Turn the dirt into mud by adding water. Prepare the area where you want your biome to grow by mining a hole and filling it with the mud.
Now, it's time to plant the seeds. Place your mushroom grass seeds on the mud. You only need one seed to start a farm, but for a larger biome, it is recommended to have around 100 blocks of blue grass in total. Place the seeds on opposite sections of the mud strip to speed up the spread. You can also try the layer platform farm design, where you create one-block-thick platforms of mud with a shaft open on one end and join them together vertically with more mud blocks. This way, one seed can cover the entire farm.
Once the seeds are planted, all you need to do is wait. It usually takes about 5-6 in-game days for the mud strip to be covered in mushroom grass. As the grass spreads, you will notice the background getting darker, and eventually, the music will change. This indicates that your glowing mushroom biome is officially ready!
Remember, creating a surface glowing mushroom biome will attract enemies, especially if you are in hardmode. A house is necessary for the Truffle NPC to spawn, so be sure to build suitable housing within your biome.
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Wait for the biome to spread
Now that you've set up your mushroom biome, it's time to wait for the mycelium to spread and colonize the substrate. This process can take some time, and the speed of colonization depends on various factors such as temperature, substrate type, and strain of mycelium. Generally, it can take upwards of 10 days for the mycelium to jump off the spawn and start growing on the substrate.
During this waiting period, it's important to maintain the right environmental conditions to encourage the growth of your mushroom biome. Make sure the temperature is optimal for mycelium growth, which is typically around 70 degrees F. Avoid temperatures above 80 degrees F, as this can introduce contamination and harm the mycelium. If you're using a fan to regulate temperature, be mindful that constant low-level fanning may not be as effective as periodically blasting new air into the environment. Exchanging all the air in the room every five to ten minutes is recommended to keep CO2 levels in check.
While you wait, you may start to see signs of mycelium growth. Keep an eye out for white mycelium threads, which indicate that colonization is taking place. The growth will initially be slow, but after about seven to eight days, you may notice exponential growth. This exponential growth phase indicates that the mycelium is actively colonizing the substrate.
If you're using mushroom grass seeds, you'll need to be patient as they spread and transform the surrounding area into a mushroom biome. It may take around 5-6 in-game days for the grass seeds to spread and cover the desired area. During this time, you can observe the background gradually darkening, and eventually, the music will change, signifying that your mushroom biome is officially established.
Remember, creating a mushroom biome requires patience and the right environmental conditions. Don't be discouraged if you don't see immediate results, as the mycelium needs time to spread and colonize. Enjoy the process of watching your glowing mushroom biome come to life!
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Build a house
Building a house in a glowing mushroom biome is a challenging but rewarding task. To begin, you will need to gather essential materials such as dirt, mud, and mushroom grass seeds. The process starts by locating a natural glowing mushroom biome and harvesting the mushrooms to obtain the seeds. It is recommended to have at least one mushroom grass seed for planting.
Once you have the seeds, the next step is to create mud by finding a source of water and turning dirt into mud. You will need approximately 250-300 blocks of mud for planting. Prepare the desired location by digging a hole and filling it with the mud you prepared. Plant your mushroom grass seeds in the mud, ensuring they are spread out to allow for adequate growth.
As the mushroom grass starts to spread and cover the mud, you can begin constructing your house. The materials used for building the house are flexible, and you can even use glowing mushrooms to create a cohesive look. The house can be decorated with glowing mushroom furniture to further enhance the aesthetic.
It is important to note that the background will gradually become darker as the mushroom grass spreads, and eventually, the music will change, indicating that the transformation into a mushroom biome is complete. This process takes time, and you may need to wait about a week for the full effect. Once your house is built and the biome is established, you can expect the Truffle NPC to spawn, adding a unique element to your glowing mushroom biome home.
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Frequently asked questions
You will need to find a natural glowing mushroom biome and harvest the mushrooms to get mushroom grass seeds. You can then plant these seeds in mud in your desired location.
You only need one seed to make a farm. However, to create a glowing mushroom biome, it is recommended to have around 100 blocks of blue grass in total.
You can make mud by finding a source of water and turning dirt into mud when standing within two blocks of the water source.
Glowing mushrooms will grow at a depth of 2ft or below.

























