
Building a mushroom laboratory at home is a great way to increase your mushroom-growing prowess. A mushroom lab is a controlled environment dedicated to cultivating mushrooms, and it can be as simple or as complicated as you want. The basic premise of a mushroom laboratory is to have a space that is clean, sterile, and free of contamination. This can be achieved with a few basic tools and some knowledge, allowing you to set up a lab and start growing mushrooms quickly. The size of the space you need depends on factors such as the number of mushrooms you aim to grow and your available resources. However, with proper planning, you can create a successful mushroom cultivation setup, whether as a hobby or a business venture.
| Characteristics | Values |
|---|---|
| Purpose | To create a controlled, clean, and sterile environment for cultivating mushrooms, experimenting with species, and developing strains |
| Location | An isolated area with low foot traffic, windows, and doors to minimize contamination; separate buildings for spawn lab and growing room |
| Size | Depends on the scale of mushroom production; a small room is sufficient for a basic lab |
| Design | Fruiting and inoculation rooms are central; advanced labs may include HEPA filters, positive pressure, and sterilization units |
| Equipment | Flow hood, pre-sterilized petri dishes, disposable gloves, mushroom grow bags, refrigerator, still air box, Martha tent, ducting installation |
| Techniques | Sterilization, inoculation, pasteurization, incubation, cloning, isolating strains, and maintaining multiple cultures |
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What You'll Learn
- Choosing a location: isolated, low foot traffic, easy to clean
- Basic equipment: laminar flow hood, fruiting chamber, Martha tent
- Reducing contamination: sterile workspaces, HEPA filters, positive pressure
- Storage: small refrigerator for long-term storage
- Design: fruiting and inoculation rooms at the centre

Choosing a location: isolated, low foot traffic, easy to clean
When choosing a location for your mushroom lab, there are several factors to consider. Firstly, it is ideal to select a space that is isolated from other areas of your home or building and has low foot traffic. This helps to minimise the risk of contamination from external sources, such as pets, children, or other people, who may inadvertently introduce contaminants into your lab space.
Secondly, the chosen location should be clutter-free and easy to clean. Smooth and hard surfaces, such as stainless steel or other non-porous materials, are preferable to carpeted or porous surfaces. This is because smooth surfaces can be easily sanitised by spraying and wiping with a decontamination solution, while porous surfaces can trap dust and potential contaminant spores, making it more challenging to maintain a sterile environment.
Additionally, consider the size of your operation and the amount of space you will need. The number of mushrooms you plan to grow and the design of your fruiting room will influence the required space. As a general guideline, you can expect to grow around 50kg of mushrooms per week in a 30m2 space. However, it is important to note that growing mushrooms is labour-intensive, and you may not want to handle this volume alone.
Lastly, access to utilities is crucial. Ensure your chosen location has access to water and electricity. You will also need to install ducting to facilitate air circulation and control humidity and temperature in your lab. Remember that growing mushrooms require a clean and sterile environment to thrive, so selecting a location that meets these criteria is essential for successful mushroom cultivation.
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Basic equipment: laminar flow hood, fruiting chamber, Martha tent
Basic Equipment for a Mushroom Lab
A mushroom lab requires a few essential pieces of equipment to create the ideal conditions for mushroom growth and to contain the process in a controlled environment. Here is a breakdown of the basic equipment you will need:
Laminar Flow Hood
The laminar flow hood is crucial for maintaining a sterile environment when working with mushroom cultures. It creates a clean, sterile airflow that prevents contaminants from entering the workspace. FP's Series I Laminar Flow Hood is an affordable option that comes largely pre-assembled. It features a high-efficiency HEPA filter, a fan, and a grounded plug and cord. Assembly only requires a screwdriver and silicone caulk, making it a convenient choice for those on a budget.
Fruiting Chamber
The fruiting chamber is where the mushrooms will grow. This enclosed space mimics the conditions and environment that different mushroom species typically grow in, helping them develop successfully. You can create a fruiting chamber using various methods, depending on the scale of your operation and the type of mushrooms you plan to grow.
For small-scale growers, a monotub is a simple option. It involves using a large plastic container partially filled with substrate and drilled with holes for ventilation. A shotgun fruiting chamber is similar but is better suited for mushrooms that grow out of bags, such as oyster or shiitake mushrooms.
If you want to grow mushrooms on a larger scale, a hydroponics tent might be a better option. You can also build a wooden stud frame covered with plastic sheeting or repurpose refrigeration shipping containers into fruiting rooms.
Martha Tent
The Martha Tent is a versatile mid-sized grow chamber that can be adapted to fit any space or budget. It provides a controlled environment for fruiting mushrooms in trays, blocks, and jars. Martha Tents can be set up in closets, kitchens, or basements and easily scaled up for larger operations. They are an excellent option for consistent mushroom growth, balancing humidity, fresh air exchange, temperature, and light.
By utilizing this basic equipment—a laminar flow hood, fruiting chamber, and Martha Tent—you can create a functional mushroom lab capable of producing consistent and high-quality mushrooms.
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Reducing contamination: sterile workspaces, HEPA filters, positive pressure
When building a mushroom lab, reducing contamination is a key consideration. A dedicated lab space makes it easier to create a clean environment, minimising the risk of contamination and increasing success rates.
Firstly, choose a location that is isolated from the rest of the house, with low foot traffic, windows, and doors to minimise opportunities for contamination. A separate building for the spawn lab and growing room is ideal, as inoculation is the main source of contaminants in mushroom farming.
To create a sterile workspace, a laminar flow hood or still air box can be used. These provide a unidirectional flow of air that has been filtered to remove airborne contamination. This is achieved using an air exchange fan, which propels the air through a HEPA filter, trapping any potential contaminants, including spores and fungi, and ensuring only clean air is circulated. HEPA filters have the highest ability to capture harmful dust, fumes, and bacteria, and are essential for maintaining the sterility of the growing environment.
To further reduce contamination, positive pressure can be added to the lab, which prevents dust, spores, and insects from entering the room through gaps and ensures all incoming air is contaminant-free. This can be achieved by installing fans and HEPA filters, creating a continuous flow of filtered clean air opposing the entry of contaminants.
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Storage: small refrigerator for long-term storage
When setting up a mushroom lab, it is important to have a small refrigerator for long-term storage. This is where you will store all your spore prints, liquid cultures, agar plates, and grain spawn. As you cultivate more mushrooms, you will naturally build up a collection of mushroom strains.
Mushrooms are delicate and perishable, and proper storage is essential to preserve their quality and flavour. The refrigerator is the ideal location for storage, but the exact spot you select can influence their longevity. It is best to avoid the crisper, as it tends to have high humidity, which is not ideal for mushrooms. Mushrooms should be kept dry to prevent spoilage.
When storing mushrooms in the refrigerator, keep them uncovered or place them in a paper bag. Paper bags are preferred as they absorb any moisture, keeping the mushrooms safe and fresh. If you don't have a paper bag, wrap the mushrooms in a paper towel and place them in a bowl in the refrigerator. Avoid plastic containers, as they can trap moisture and speed up spoilage.
Dry mushrooms are best kept in a deep freeze. Freezing and dehydrating mushrooms are effective methods for long-term preservation. To dry mushrooms, you can use a dehydrator or air-drying techniques, which involve placing the mushrooms in a mesh container with good airflow for about a week. Once they are completely dry, store them in an airtight glass jar.
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Design: fruiting and inoculation rooms at the centre
The fruiting and inoculation rooms are central to the design of a mushroom farm or laboratory. The fruiting room is where mushrooms grow and develop, while the inoculation room is where the spawn is prepared. These rooms should be at the heart of the building to minimise the risk of contamination.
The fruiting room should be kept at a negative pressure to prevent the dispersal of mushroom and contaminant spores into other areas of the farm or laboratory. It should be well-ventilated, with good airflow and air circulation. Relative humidity should be maintained at around 80-90% to encourage mushroom growth. This can be achieved through misting and the use of fans. Light and temperature should also be controlled, with a 12-hour day and night cycle recommended.
The inoculation room, meanwhile, should be a sterile environment, free from contaminants. It should be a sealed space with easy-to-clean hard surfaces, such as stainless steel tables and smooth floors, to facilitate decontamination. A flow hood is essential for this room, as it creates a sterile airflow within which to work.
In terms of equipment, the inoculation room should contain pre-sterilised and disposable petri dishes, preferably made of glass so they can be reused. A small refrigerator is also necessary for long-term storage of spore prints, liquid cultures, agar plates, and grain spawn.
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Frequently asked questions
A mushroom lab is a controlled environment dedicated to cultivating mushrooms. It’s a clean, sterile space where mushroom growers can experiment with different mushroom species, develop new strains, and produce high-quality mushroom mycelium.
You will need a laminar flow hood, liquid culture, basic tools, a fruiting chamber, and a Martha tent. You will also need pre-sterilised and disposable petri dishes, a small refrigerator, and a still-air box.
Ideally, you want a space that is isolated from the rest of your house and has low foot traffic, windows and doors to minimise opportunities for contamination.
The size of the space you need depends on how many mushrooms you’re aiming to grow, how you build your fruiting room and what options are available to you. As a guide, if you optimised everything well, you’d be able to grow up to 50kg of mushrooms a week in a space of around 30m2.

























