Glowing Mushrooms: Cultivating Your Own Bioluminescent Fungi

how to cultivate bioluminescent mushrooms

Bioluminescent mushrooms are a fascinating natural phenomenon, with over 70 species of fungi that can glow in the dark. The process of growing these mushrooms at home involves patience and precision. The basic procedure is similar to cultivating other wood-decaying mushrooms, but the slow growth of bioluminescent species requires specific conditions to prevent mould. This guide will explore the steps to cultivate these glowing mushrooms, from preparing the growing medium to maintaining optimal temperatures and humidity for successful fruiting.

Characteristics Values
Mushroom Species Panellus Stipticus, Lions Mane, Ghost Fungus, Jack-O-Lantern, Omphalotus nidiformis, Mycena chlorophos, Armillaria mellea, Armillaria gallica, Mycena pura, Neonothopanus gardneri
Growing Medium Sterilized blocks of wood shavings, wood supplemented cake, rye grain pocket, hardwood substrate, brown rice flour, logs of wood
Temperature Range 65-80° Fahrenheit
Incubation Temperature 65-80° Fahrenheit
Fruiting Temperature 60-75° Fahrenheit
pH Range 3.5-4 for optimum bioluminescence
Air Requirements Fresh air, high humidity, frequent air exchange
Light Requirements Darkness
Growth Cycle 22-hour cycle that corrects to 24 hours based on temperature
Growth Duration 6-7 months
Edibility Not edible

anspore

Choosing a species: Panellus stipticus is one of the brightest bioluminescent mushrooms

Choosing a species is an important step in cultivating bioluminescent mushrooms. One of the brightest bioluminescent mushrooms is Panellus stipticus, commonly known as the bitter oyster, the astringent panus, the luminescent panellus, or the stiptic fungus. This species of mushroom is native to the US and is mostly found on the East Coast on deciduous hardwood logs and stumps. The gills of the Glowing Panellus shine brightly in complete darkness, making it a fascinating choice for those interested in cultivating bioluminescent mushrooms.

Panellus stipticus is known for its ability to produce light through a chemiluminescence reaction. This reaction involves treating specific compounds within the fungus with salt of ammonia or primary amines, along with iron(II), hydrogen peroxide, and a cationic surfactant. The level of light emission is regulated by the enzyme superoxide dismutase (SOD), which plays a critical role in the brightness of the bioluminescence.

When choosing Panellus stipticus for cultivation, it is important to note that this species has a preference for specific environmental conditions. It thrives in northern temperate regions and has been found in Europe, Australia, New Zealand, Anatolia, Japan, China, and Alaska. In North America, it is more commonly found in the eastern regions, with a southern range extending to Costa Rica. The mushroom typically grows on decaying wood, particularly hardwood species such as birch.

To cultivate Panellus stipticus, one can follow a similar process to growing other wood-decaying mushrooms. However, it is important to note that bioluminescent mushrooms have extremely slow growth, so certain techniques like grain spawn may not be effective due to mould issues. Here is a general guide for cultivating Panellus stipticus:

  • Prepare a suitable growing medium: Choose a hardwood log or block of wood shavings, preferably from a water wood species like willow or poplar. Sterilize the wood to ensure it is free from contaminants, as they can negatively impact bioluminescence.
  • Obtain spawn: Purchase pre-cultured Panellus stipticus spawn online or from a reputable supplier.
  • Inoculate the growing medium: Drill holes into the wood and insert the spawn. Alternatively, you can use mycelium pegs or colonize a bioluminescent mushroom wood log.
  • Provide optimal conditions: Place the inoculated wood in a bright location but avoid direct sunlight. Maintain temperatures between 65 to 80 degrees Fahrenheit, as this type of mushroom thrives in fresh air and adequate moisture.
  • Monitor development: Check the development of the mycelium daily. After about two weeks, cut open the top of the bag or growing container when you observe white fluffy growth.
  • Add moisture: Pour 1/4 cup of distilled water into the container and distribute it throughout the growing medium. Close the bag and place it back into the growing chamber for about a week.
  • Expose to outdoor conditions: Once the entire bag is colonized with white mycelium, move the container outdoors. Maintain temperatures between 60 to 75 degrees Fahrenheit and expose the colonized substrate to fresh air.
  • Observe fruiting bodies: After several weeks to months, depending on the growing medium, you will observe the formation of mushroom caps. Transport the fully developed fruiting bodies to a dark area to observe their bioluminescence.

anspore

Growing conditions: Bioluminescence is highly nitrogen and pH-dependent

Bioluminescence in both nambi and stipticus is highly nitrogen and pH-dependent. To achieve optimum bioluminescence, it is recommended to use an acid buffer to adjust the pH to a range of 3.5 to 4. At a pH level of 6 to 7, no bioluminescence will be visible.

Nitrogen sources play a key role in the growth of mushrooms. For instance, yeast extract, a rich source of protein, amino acids, and vitamins, has been found to promote the growth of mycelium in some mushroom species. Dextrose and glycine, when used together, also support the maximum vegetative growth of edible mushrooms.

When cultivating bioluminescent mushrooms, it is important to maintain a temperature range of 65 to 80 degrees Fahrenheit. These mushrooms thrive in fresh air, so ensure the growing environment is well-ventilated. The growth of bioluminescent mushrooms is a slow process, and they are susceptible to mould due to the lengthy growth period. Therefore, it is recommended to use a sterile substrate and inoculate it with a mix of hardwood chips and oat flakes, transferring it to a bulk substrate after four to six weeks.

Bioluminescent mushrooms, such as the Jack-O'-Lantern variety, are known for their glowing appearance due to a chemical reaction involving luciferase and oxygen. This phenomenon, called "foxfire," typically occurs in mushrooms growing on decaying wood. The light they emit helps attract insects that aid in spore dispersal.

anspore

Temperature: Maintain a temperature range of 65–80°F

Temperature is a critical factor in cultivating bioluminescent mushrooms successfully. Maintaining the optimal temperature range is essential to encourage mushroom growth and bioluminescence.

Bioluminescent mushrooms generally thrive within a temperature range of 65–80°F (18.3–26.7°C). This range is ideal for the incubation period, promoting the development of the mycelium and the subsequent fruiting of the mushrooms. Deviating significantly from this range may hinder the growth of the mushrooms and their ability to glow.

The specific temperature requirements may vary slightly depending on the species of bioluminescent mushroom being cultivated. For example, Mycena chlorophos, a species native to Indonesia, Japan, Sri Lanka, Australia, and Brazil, exhibits its brightest glow when the temperature reaches around 80°F (26.7°C). This temperature aligns with its native subtropical climate.

Additionally, it is important to note that mushrooms tend to glow on a cycle, similar to the human body's circadian rhythm. They maintain a 22-hour cycle that corrects to 24 hours based on temperature. Therefore, maintaining a consistent temperature within the optimal range can help synchronize their bioluminescent rhythm.

The temperature preferences of bioluminescent mushrooms also extend beyond their incubation and growth stages. Once the mushrooms have reached maturity, they can be moved outdoors, where they will continue to thrive within a slightly narrower temperature range of 60–75°F (15.6–23.9°C). This adjustment in temperature can induce fruiting and expose the mushrooms to the fresh air they relish.

anspore

Light observation: Place in a dark room to observe the bioluminescence

Once your bioluminescent mushrooms have grown, you can observe their light in a dark room. The mushrooms will glow in the dark due to a chemical reaction between oxyluciferin molecules, an enzyme called luciferase, and oxygen.

To observe the bioluminescence, place the mushrooms in a dark room and allow your eyes time to adjust to the darkness. You may need to wait until nightfall to see the full effect. The mushrooms will likely appear as a constant greenish-blue light, increasing in brightness over time. This is because mushrooms tend to glow on a cycle, similar to the human body's circadian rhythm, and they will intensify their glow at night when it is dark and most effective.

The brightness of the bioluminescence can also depend on air pressure, temperature, and pH levels. For example, Mycena chlorophos is brightest when it is one day old and the temperature is around 80 degrees Fahrenheit, while Armillaria mellea only glows in its mycelia, a part of the mushroom that is usually invisible.

If you want to capture the bioluminescence with photography, you will need a camera that allows you complete control over the settings. You can take a "daytime" photo and a bioluminescent photo to create a before-and-after comparison. Using a tripod is good practice for both daytime and nighttime photography.

anspore

Fruiting bodies: Expect to see little mushroom caps after several months

Bioluminescent mushrooms are a fascinating sight, and cultivating them can be a rewarding experience. One of the key steps in this process is observing the development of fruiting bodies, which are the little mushroom caps that form after several months of care and patience.

First, let's understand the life cycle of these glowing mushrooms. It all starts with the mycelium, the part of the fungus that is responsible for nutrient absorption and growth. The mycelium will gradually colonize the growing medium, and this stage can take a few weeks to ensure complete colonization. During this time, it's important to maintain the right temperature and provide fresh air by fanning the bag and cutting it open when necessary.

After the mycelium has fully colonized the substrate, it's time to move the container outdoors or to an area with the right temperature and humidity levels. This step is crucial for the next phase of development—the formation of fruiting bodies. The mycelium will start to produce little mushroom caps, and this process can take several months. It's important to be patient and provide the necessary moisture and temperature control during this period.

As the months go by, keep an eye out for the emergence of tiny mushroom caps. These caps are the fruiting bodies, and they signal that your bioluminescent mushrooms are nearing maturity. The caps will usually stay for several weeks, providing you with a wonderful display of nature's beauty. With a good hardwood substrate, you may even enjoy mushroom caps for several years!

To optimize the growth of fruiting bodies, consider using a hardwood fruiting block. This block takes around three to four months to prepare for fruiting, but it can provide a more sustainable base for your mushrooms. Additionally, maintaining the right temperature and humidity levels is crucial. Bioluminescent mushrooms thrive in temperatures ranging from 65 to 80 degrees Fahrenheit during incubation and fruiting.

Finally, when the mushroom caps have fully developed, it's time to move them into a dark area. This step will allow you to observe the mesmerizing green glow of bioluminescence. The contrast between the dull shade of the mushrooms during the day and their brilliant glow in the dark is truly remarkable.

Frequently asked questions

Some bioluminescent mushrooms include Lions Mane, Ghost Fungus, Jack-O-Lantern, Panellus stipticus, Panellus pusillus, Armillaria mellea, Armillaria gallica, Mycena chlorophos, Mycena pura, and Omphalotus nidiformis.

Bioluminescence in mushrooms is highly nitrogen and pH-dependent. The pH should be adjusted to between 3.5 and 4 for optimum bioluminescence. The ideal temperature range is between 65 to 80 degrees Fahrenheit. The mushrooms should be kept in an area with fresh air and high humidity.

First, purchase spawn and a prepared mushroom grow bag with a rye grain pocket from a mushroom supplier. Cut open the top of the bag when the mycelium has colonized the grain pocket with white fluffy growth. Add 1/4 cup of distilled water and seal the bag. Place it back into the growing chamber for about a week. Open the bag and observe the development of the mycelium daily. When the entire bag is colonized with white mycelium, move the container outdoors.

Written by
Reviewed by

Explore related products

Share this post
Print
Did this article help you?

Leave a comment