
Psilocybin mushrooms, also known as magic mushrooms or shrooms, are classified as a Schedule I drug and have been the subject of increasing interest in psychedelic research. Despite the legal restrictions on their use, there is a growing body of evidence that suggests psilocybin mushrooms may have therapeutic benefits for mental health disorders, including post-traumatic stress disorder, depression, anxiety, and addiction. As a result, Oregon became the first state to legalize the adult use of psilocybin mushrooms in 2020, leading to a surge of investment in psychedelic research. This trend has sparked interest in cultivating psilocybin mushrooms, with some universities receiving licenses to grow them for research purposes. While the potential medical benefits of psilocybin mushrooms are being explored, it is important to remember that their use outside of medical supervision can carry risks, and adverse effects such as bad trips have been associated with their consumption.
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What You'll Learn

Choosing the right spores
First, it is important to understand that not all mushroom species can be cultivated artificially. Some mushrooms only grow in their natural wild habitat, so make sure the species you choose is suitable for cultivation.
When selecting spores, opt for a reputable and trusted supplier with extensive knowledge of mycology and spore cultivation. They should be able to provide guidance and advice on the specific requirements of the spores they offer. Look for suppliers who prioritize the purity and viability of their spores, as this will increase your chances of successful cultivation.
Consider the substrate on which the spores will grow. Different species of mushrooms have specific substrate preferences, so choose spores that align with the substrate you plan to use. For example, some mushrooms thrive on wood chips, sawdust, or straw, while others prefer compost or manure-based substrates. Matching the spores to their ideal substrate will encourage vigorous growth.
Additionally, pay attention to the climate and environmental conditions required for the spores' germination and fruiting. Psilocybin mushrooms typically prefer specific temperature and humidity ranges, so choose spores that align with the conditions you can provide. This ensures that your cultivation efforts are more likely to succeed.
Lastly, always prioritize your safety when working with psilocybin mushroom spores. Wear protective gear, including gloves and a face mask, when handling the spores and growing medium. Ensure proper ventilation in your workspace, and refrain from ingesting any mushrooms unless you are absolutely certain of their safety.
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Preparing the substrate
Selecting the Right Materials
The substrate provides the nutrients necessary for mushroom growth, so choosing the right materials is essential. Various materials can be used as a substrate, including straw, wood chips, gypsum, and manure. It is important to ensure that the materials are free from contaminants and have the right nutritional composition for mushroom growth.
Sterilization and Pasteurization
To create a sterile environment for the mycelium to grow, sterilization and pasteurization processes are necessary. This step helps prevent contamination and ensures the substrate is free from harmful bacteria and microorganisms that could hinder mushroom growth. Sterilization can be achieved through pressure cooking or steaming, while pasteurization involves heating the substrate to a lower temperature to reduce the number of contaminants.
The chosen substrate materials need to be properly mixed and hydrated to create the ideal growing environment. This usually involves soaking the materials in water and then draining them to ensure they are moist but not soggy. The substrate mixture should be fluffy and airy, providing adequate oxygen and moisture retention for the mycelium to proliferate.
PH Adjustment
Maintaining the right pH level in the substrate is critical for mushroom growth. Most mushrooms prefer a slightly acidic environment, so adjusting the pH to a range of 5.5 to 6.5 is generally recommended. This can be achieved by adding lime or other pH adjusters to the substrate mixture. Be sure to test the pH regularly to ensure it remains within the optimal range.
Packing the Substrate
Once the substrate mixture is ready, it needs to be packed into containers or bags, commonly known as spawn bags. These containers should be sterile and have proper ventilation to allow gas exchange during mushroom growth. Make sure to pack the substrate loosely, avoiding compaction, as this can hinder the growth of the mycelium.
Inoculation
Inoculation is the process of introducing the mushroom spawn or culture to the prepared substrate. This step involves mixing the spawn evenly into the substrate to ensure colonization. It is important to work quickly and sterilely during inoculation to prevent contamination. After inoculation, the substrate needs to be sealed and maintained within the appropriate temperature and humidity ranges to encourage mycelium growth.
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Sterilising the equipment
Sterilising equipment is a crucial step in cultivating psilocybin mushrooms, as contamination is the most common source of failure in any mushroom lab. The mycelium must colonise the entire substrate to develop healthy fruiting. However, if the material used is contaminated, microorganisms will compete with the mycelium for nutrients, hindering its growth.
There are various methods for sterilising equipment, and the right one depends on the type of material and the context of cultivation. Steam sterilisation, using equipment like autoclaves, is the most effective and reliable way to sterilise mushroom substrate. Autoclaves are industrial equipment designed specifically for steam sterilisation, offering greater capacity and additional features like vacuum systems compared to pressure cookers. They are widely used in labs due to their precision and ability to control the preparation process, resulting in consistent results and higher yields.
For those without access to autoclaves, pressure cookers or atmospheric pressure steriliser units are viable alternatives for sterilisation. Pressure cookers are commonly used by mushroom hobbyists, but their limited capacity makes them impractical for larger operations. Atmospheric pressure steriliser units can be a step up from pressure cookers for those seeking more advanced equipment.
The sterilisation process should be tailored to the specific needs of the mushroom cultivation. In some cases, dry heat may be used for non-straw mushroom substrates, but this requires subsequent rehydration of the substrate. Repeating the sterilisation process may be necessary if biological indicators (BIs) remain active after the initial cycle.
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Inoculating the substrate
Preparing the Substrate
Before inoculating the substrate, it is essential to prepare the growing medium. Commonly used substrates for psilocybin mushrooms include sterilized rye grain or a mixture of vermiculite, perlite, and gypsum, known as "casing soil." Prepare your substrate by following these steps:
- If using rye grain, purchase food-grade rye grain and sterilize it by pressure cooking. This kills any contaminants and prepares the grain for optimal mushroom growth.
- For casing soil, mix vermiculite, perlite, and gypsum in equal parts. Sterilize the mixture by steaming or boiling to eliminate any unwanted microorganisms.
- Prepare your containers by cleaning and sterilizing them. Jars or containers with wide openings are ideal for rye grain, while shallow trays or containers are suitable for casing soil.
Spawning and Inoculation
Once your substrate is prepared, it's time to introduce the mushroom spawn:
- Wear sterile gloves or wash your hands thoroughly to avoid contaminating the spawn and substrate.
- If using rye grain, fill your containers with the sterilized grain, leaving some space at the top. Allow the grain to cool to room temperature before use.
- For casing soil, spread the substrate evenly in your trays or containers to a depth of 2-3 inches. Lightly water the substrate to maintain moisture.
- Obtain your mushroom spawn, choosing a strain suitable for your growing conditions and preferences.
- For rye grain, carefully sprinkle the spawn over the surface of the grain, mixing gently to ensure even distribution.
- With casing soil, create small indentations or holes in the substrate and place small amounts of spawn in each hole. Cover the spawn with a thin layer of the substrate.
Post-Inoculation Care
After inoculation, proper care is essential to encourage mycelium growth:
- Seal the containers with micropore tape or breathable fabric to allow gas exchange while preventing contamination.
- Maintain a suitable temperature, typically between 70-80°F (21-27°C), and keep the substrate moist, misting with water if needed.
- Store your containers in a dark place, such as a closet or a covered area with indirect light.
- Monitor your substrate regularly for signs of contamination, such as unusual colours or odours.
By following these steps, you can successfully inoculate your substrate and create an optimal environment for psilocybin mushroom growth. Remember to work hygienically and maintain sterile conditions throughout the process to increase your chances of a healthy mushroom crop.
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Maintaining the right environment
Temperature and Humidity Control: Psilocybin mushrooms thrive in a temperature range of 70-80°F (21-27°C). Aim to maintain a relative humidity of 90-95% during the initial stages of growth. You can adjust the humidity down to 70-80% as the mushrooms start to pin and develop. Use a thermostat-controlled heater or air conditioner to regulate temperature and a humidifier or dehumidifier to control humidity levels.
Ventilation and Airflow: Proper airflow is essential for mushroom growth and to prevent the buildup of excess moisture, which can lead to mould or bacteria. Use fans to create a gentle airflow, ensuring fresh air is circulated throughout the growing area. Avoid excessive airflow, as this can dehydrate the mushrooms.
Light and Darkness: Psilocybin mushrooms require a balance of light and darkness. During the initial stages, maintain a dark environment with minimal light exposure. As the mushrooms develop, provide indirect light, avoiding direct sunlight, which can be too intense. Aim for 12 hours of light and 12 hours of darkness each day.
Substrate Moisture: The growing substrate, usually a mixture of nutrients and organic matter, should be moist but not soaked. Ensure the substrate is well-drained to prevent waterlogging, which can cause anaerobic conditions harmful to the mushrooms. Regularly check the moisture content and adjust by spraying water or providing additional drainage if needed.
Cleanliness and Sanitation: Sanitation is critical to prevent contamination. Sterilize all equipment and tools before use and maintain a clean growing environment. Use sterile gloves when handling substrates and mushrooms to avoid introducing contaminants. Regularly inspect your mushrooms for any signs of mould or bacteria, removing affected areas immediately to prevent the spread.
By carefully controlling these environmental factors, you can create optimal conditions for cultivating psilocybin mushrooms. Remember that consistency is key, and any deviations from the ideal environment may impact the success of your cultivation.
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