
Growing mushrooms is an exciting yet challenging process that requires keen observation and understanding of the various stages of mushroom development. One of the critical steps in mushroom cultivation is knowing when and how to cut the grow bags to ensure optimal growth and yield. The timing of cutting the bags depends on several factors, including the colonisation process, environmental conditions, and signs of healthy mushroom growth.
| Characteristics | Values |
|---|---|
| When to cut the bag | When full colonisation is evident, with factors such as temperature and humidity playing a significant role. Mycelium should appear white and fluffy, indicating robust colonisation. |
| How to cut the bag | Cut a slit 1-2 inches above the substrate matching the width of the filter patch. Once pins have formed, extend the cut line across the front to introduce more fresh air exchange. |
| Additional tips | The ideal temperature range is between 75-80 degrees Fahrenheit, with a humidity level of 95-100%. Sanitise your workspace, scissors, or utility knife, as well as the bag. Maintain a sterile environment to avoid contamination. |
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What You'll Learn

When to cut the bag open
The timing of when to cut open a mushroom bag is crucial for optimal growth and a bountiful yield. The ideal time to cut open the bag is when full colonisation is evident, indicated by a white, fluffy, dense layer of mycelium across the surface of the substrate. This phase is critical as it sets the foundation for healthy mushroom development.
It is essential to monitor the growth closely and look for any signs of contamination. A lack of discoloration or foul odour indicates healthy growth. The ideal temperature range for most species is between 75-80 degrees Fahrenheit, with a humidity level of 95-100%. It is also crucial to ensure that the bag is kept in a space with indirect light, as direct sunlight can inhibit mycelium growth.
Once full colonisation is achieved, the mycelium network will be robust enough to withstand the introduction of fresh air and humidity that comes with opening the bag. At this stage, you can cut a small 5cm long incision or slit 1-2 inches above the substrate, matching the width of the filter patch. This will allow for the necessary fresh air exchange while maintaining high levels of humidity.
For fast-growing species such as oysters, it is recommended to cut the full hood as soon as pins have formed. You can either fold it back or close it to trap more moisture within the growing environment if necessary. As the mushrooms continue to develop, you should cut a hood into the bag by extending the cut line around the sides while leaving the back intact.
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Preparing the bag
Sterilize the Environment and Materials
Start by sterilizing your workspace. Use 70% isopropyl alcohol to wipe down all surfaces, including your scissors or utility knife, and the mushroom bag itself. Allow the bag to sit in the sanitized workspace for several seconds, then wipe it dry with a clean paper towel. Put on gloves and sanitize your hands with isopropyl alcohol as well. This step is crucial to reducing the chances of contamination during the mushroom growth process.
Prepare the Mushroom Bag
To prepare the mushroom bag itself, you will need sterilized grain and mushroom genetics. Using a sterile needle, inject 2-3ml of a spore syringe directly through the back of the bag. Seal the injection hole with micropore tape. The grain should be sterilized grain, such as oats or rye berries, that has been inoculated and fully colonized by mushroom mycelium. Cover the injection hole securely.
Store in Optimal Conditions
Store the inoculated bags in a dark, warm, and humid environment. The ideal temperature range is between 75-80 degrees Fahrenheit, with a humidity level of 95-100%. Ensure the space has indirect light, as direct sunlight can inhibit mycelium growth. The incubation process can take between 1 to 3 weeks, depending on the colonized grain-to-mushroom substrate ratio. During this time, the white mushroom mycelium will grow and spread over the fruiting substrate.
Monitor for Signs of Healthy Growth
Keep a close eye on the bag during incubation. The mycelium should appear white and fluffy, indicating robust colonization. There should be no discolouration or foul odour, as these suggest contamination. Once the mycelium has fully colonized the substrate, you will know it is ready for the next step.
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Cutting the bag
Before cutting the bag, it is important to understand the stages of mushroom development and create optimal conditions for mycelium growth. The ideal temperature range is between 75-80 degrees Fahrenheit, with a humidity level of 95-100%. Indirect light is also preferable, as direct sunlight can inhibit mycelium growth. Sterilizing your workspace and equipment is crucial to reducing contamination risks. Use gloves and masks, and wipe down surfaces with 70% isopropyl alcohol.
The best time to cut the bag is when full colonization is evident. You will see white and fluffy mycelium spreading over the substrate, indicating robust colonization. This process can take between 1 to 3 weeks, depending on the conditions and the mushroom variety. Once full colonization is achieved, the substrate will appear white and dense, and this is a sign that the bag is ready for the next stage.
When you are ready to cut the bag, start by sanitizing your scissors or utility knife and the bag itself. Cut a slit 1-2 inches above the substrate, matching the width of the filter patch. If using an enclosed grow chamber, you can place the bag inside. Then, let the bag sit until primordia or "pins" (baby mushrooms) appear. This usually takes about a week but can vary depending on environmental conditions.
Once the pins have formed, extend the cut line across the front of the bag to introduce more fresh air exchange. For fast-growing species like Oysters, cut the full hood as soon as pins appear. You can fold it back or close it to trap moisture within the growing environment if needed. As the mushrooms continue to develop, cut a hood into the bag by extending the cut line around the sides while leaving the back intact.
Remember, mushroom cultivation is a delicate process that requires keen observation and understanding of the various stages of development. By following these steps and maintaining optimal conditions, you can successfully cut the bag and encourage healthy mushroom growth.
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After cutting the bag
To increase humidity, spray the sides and up into the hood of the bag several times over a few hours. This gives the moisture time to be absorbed rather than pooling at the bottom. Close the bag by tilting the hood down, trapping in the CO2 and humidity needed for a second flush of growth.
It is also important to regularly monitor the mushrooms for any signs of contamination. Indicators of healthy growth include white and fluffy mycelium, with no discolouration or foul odour. If there are signs of stagnant growth or contamination, review the growing conditions and consult experienced cultivators.
Once the mushrooms are ready to harvest, cut the bottom portion of each stem and ensure there is no growing medium remaining on the mushroom. Dry your mushrooms using your preferred method, and repeat the process for the second and third flushes. After the third flush, the grow bag can be disposed of.
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Avoiding contamination
Maintain a Clean Environment: It is essential to maintain a sterile environment around your mushroom bags to prevent contamination. Sanitize your hands and tools before coming into contact with the inside of the bags. Use gloves, masks, and alcohol to sterilize your equipment and surroundings.
Proper Sterilization: Sterilization of mushroom bags is crucial. Steam sterilization, pressure cooker sterilization, and chemical sterilization are common methods to ensure your bags are free from contamination. Choose a method considering the scale of your operation, the type of substrate, and available resources.
Monitor for Signs of Contamination: Regularly inspect your mushroom bags for any signs of contamination. Look for unusual colors, odors, or stagnant growth. Healthy mycelium should appear white and fluffy, indicating robust colonization.
Timing of Bag Opening: Do not open the mushroom bag until the mycelium has fully colonized the substrate. Opening the bag too early increases the risk of contamination. Wait until the bag appears white and fluffy with mycelium, and there are no signs of discoloration or foul odors.
Air Exchange: Mushrooms require fresh air exchange, but be cautious when exposing the bag's contents. If your bag has a filter patch, avoid cutting it. Instead, gently squeeze the top of the bag and pull to allow fresh air in. If you choose to cut slits in the bag, make sure they are small to prevent excessive air exchange, which can impact mushroom growth.
Harvesting Techniques: When harvesting mushrooms, use proper techniques to minimize the risk of contamination. Hand-picking is recommended; gently twist the mushroom stem until it separates from the substrate. Avoid pulling or using a knife, as this can damage the substrate and increase the risk of contamination.
By following these steps and maintaining a sterile environment, you can significantly reduce the risk of contamination when cutting mushroom bags.
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Frequently asked questions
You should cut open the bag when pinning has begun. This is when “pins”, or the first fruiting bodies, start to grow.
The substrate will appear fully white and dense with mycelium, indicating that colonisation is complete.
Sanitise your workspace, scissors or utility knife, and the bag. You should also put on gloves and clean with isopropyl.
Cut a slit 1-2 inches above the substrate, matching the width of the filter patch. Once pins have formed, extend the cut line across the front of the bag to introduce more fresh air exchange.
Add humidity to the growing environment by spraying the sides and up into the hood of the bag. Then, close the bag by tilting the hood down to trap in the CO2 and humidity needed for a second flush.

























