
Growing mushrooms from stem butts is a fascinating and sustainable method that allows you to cultivate new fungi using the leftover bases of harvested mushrooms. This technique is particularly popular with oyster mushrooms but can also work with other varieties. To begin, ensure the stem butt is fresh and healthy, as it contains the mycelium necessary for growth. Prepare a suitable growing medium, such as straw, sawdust, or coffee grounds, sterilize it to prevent contamination, and inoculate it with the stem butt. Maintain optimal conditions—high humidity, proper ventilation, and a consistent temperature—to encourage mycelium colonization. With patience and care, the stem butt will sprout new mushrooms, offering a rewarding and eco-friendly way to enjoy homegrown fungi.
| Characteristics | Values |
|---|---|
| Substrate | Straw, wood chips, sawdust, or compost (sterilized or pasteurized) |
| Stem Butt Source | Freshly harvested mushroom stem butts (preferably from oyster mushrooms) |
| Sterilization/Pasteurization | Required for substrate to eliminate competing organisms |
| Moisture Level | 50-70% moisture content in substrate |
| Temperature Range | 60-75°F (15-24°C) for incubation |
| Humidity | 85-95% relative humidity |
| Light Requirements | Indirect light or low-light conditions |
| Incubation Period | 2-4 weeks for mycelium colonization |
| Fruiting Conditions | Cooler temperatures (55-65°F or 13-18°C) and high humidity |
| Harvest Time | 5-14 days after pinning (small mushroom formation) |
| Yield per Butt | Varies; typically 1-3 flushes of mushrooms per stem butt |
| Common Mushroom Types | Oyster, shiitake, and other saprotrophic mushrooms |
| Contamination Risks | Mold, bacteria, or competing fungi if not sterilized properly |
| Reusability of Substrate | Limited; substrate degrades after 1-2 harvests |
| Difficulty Level | Moderate; requires attention to sterilization and humidity control |
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What You'll Learn
- Prepare the Substrate: Sterilize sawdust or straw, mix with nutrients, and moisten for optimal mushroom growth
- Inoculate with Spawn: Introduce mushroom mycelium to the substrate, ensuring even distribution for colonization
- Maintain Humidity: Keep the environment humid (85-95%) using misting or a humidifier for healthy growth
- Control Temperature: Maintain 65-75°F (18-24°C) to encourage mycelium development and fruiting
- Harvest and Repeat: Pick mature mushrooms, rehydrate the butt, and restart the cycle for multiple yields

Prepare the Substrate: Sterilize sawdust or straw, mix with nutrients, and moisten for optimal mushroom growth
To prepare the substrate for growing mushrooms from a stem butt, the first step is to sterilize the sawdust or straw. This is crucial to eliminate any competing organisms that could hinder mushroom growth. Start by placing the sawdust or straw in a large pot or container. Pour boiling water over the material, ensuring it is fully submerged, and let it sit for at least an hour. Alternatively, you can use a pressure cooker or autoclave to sterilize the substrate at 121°C (250°F) for 30-60 minutes. Proper sterilization ensures a clean environment for the mushroom mycelium to thrive.
Once the sawdust or straw is sterilized, allow it to cool to a temperature that is safe to handle, typically around 25-30°C (77-86°F). Next, mix in the necessary nutrients to support mushroom growth. Common supplements include wheat bran, cornmeal, or gypsum, which provide essential elements like nitrogen and minerals. A general ratio is to mix 5-10% of the nutrient supplement by weight with the sterilized sawdust or straw. Thoroughly combine the ingredients to ensure an even distribution, as this will promote consistent mycelium colonization.
After mixing the nutrients, it’s essential to moisten the substrate to the correct humidity level. The goal is to achieve a moisture content of around 60-70%, which feels like a wrung-out sponge—damp but not soggy. Add clean, preferably distilled or filtered water gradually while mixing the substrate. Avoid overwatering, as excess moisture can lead to anaerobic conditions and contamination. Proper moisture levels are critical for the mycelium to grow efficiently and for the mushrooms to develop healthily.
Once the substrate is sterilized, nutrient-enriched, and properly moistened, it’s ready for inoculation with the mushroom stem butt. Fill sterilized growing containers or bags with the prepared substrate, leaving enough space for the mycelium to expand. Insert the stem butt into the substrate, ensuring good contact with the material. Seal the container or bag to maintain humidity and prevent contamination. Place the setup in a dark, cool environment (around 22-25°C or 72-77°F) to encourage mycelium growth. Regularly monitor the substrate for signs of contamination and adjust conditions as needed for optimal mushroom development.
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Inoculate with Spawn: Introduce mushroom mycelium to the substrate, ensuring even distribution for colonization
To successfully grow mushrooms from a stem butt, the inoculation process is critical. Begin by preparing your spawn, which is the mushroom mycelium that will colonize the substrate. You can purchase pre-made spawn or create your own by transferring mycelium from a healthy stem butt to a sterile grain substrate. Ensure the spawn is fully colonized and active before proceeding. The goal is to introduce this mycelium into the stem butt or prepared substrate in a way that promotes even and thorough colonization.
Once your spawn is ready, prepare the stem butt by cleaning it thoroughly to remove any dirt or debris. If using a wooden substrate, ensure it is properly hydrated and free of contaminants. Create small holes or grooves in the stem butt using a drill or knife, spacing them evenly to allow the mycelium to spread. These holes serve as entry points for the spawn, encouraging the mycelium to grow into the substrate. Avoid overcrowding the holes, as this can hinder proper colonization.
Next, introduce the spawn into the holes or onto the surface of the substrate. If using grain spawn, break it into small pieces and gently pack it into the holes, ensuring good contact between the spawn and the substrate. For stem butts, you can also place a layer of spawn on the cut surface and cover it lightly with soil or another substrate to retain moisture. The key is to distribute the mycelium evenly to prevent uneven growth and ensure the entire substrate is colonized.
After inoculation, maintain optimal conditions for mycelium growth. Keep the substrate in a warm, humid environment, typically around 70-75°F (21-24°C), and protect it from direct light. Mist the area lightly to maintain moisture, but avoid overwatering, as excess moisture can lead to contamination. Cover the inoculated stem butt with a plastic bag or place it in a humid container to create a mini-greenhouse effect, which helps retain humidity and supports mycelium growth.
Monitor the substrate regularly for signs of colonization. Healthy mycelium will appear as white, thread-like growth spreading through the substrate. If mold or other contaminants appear, remove the affected areas immediately to prevent further spread. With proper inoculation and care, the mycelium will fully colonize the stem butt within a few weeks, setting the stage for mushroom fruiting. Patience and attention to detail during this step are essential for a successful harvest.
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Maintain Humidity: Keep the environment humid (85-95%) using misting or a humidifier for healthy growth
Maintaining the correct humidity level is crucial when growing mushrooms from a stem butt, as it directly impacts the mycelium’s ability to thrive and fruit. Mushrooms require a highly humid environment, typically between 85% and 95%, to prevent the mycelium from drying out and to encourage healthy growth. Without adequate humidity, the stem butt may fail to develop new mushrooms, or the existing mycelium could weaken and die. To achieve this, you can use a humidifier in your growing area, ensuring it consistently maintains the desired range. Place the humidifier near the growing container but not so close that it causes water to pool on the substrate, which could lead to contamination.
Misting is another effective method to maintain humidity, especially if you’re working with a smaller setup. Use a fine-mist spray bottle to lightly mist the air around the stem butt and its growing environment several times a day. Avoid misting the stem butt directly, as excessive moisture on its surface can lead to mold or bacterial growth. Instead, focus on increasing the ambient humidity by misting the walls of the growing chamber or the air above the substrate. Be consistent with misting, as fluctuations in humidity can stress the mycelium and hinder growth.
If you’re using a growing chamber or container, such as a plastic tub or a terrarium, ensure it has proper ventilation while still retaining moisture. Cover the container with a clear lid or plastic wrap to create a mini greenhouse effect, trapping humidity inside. Periodically check the humidity levels using a hygrometer to ensure they remain within the 85-95% range. If the humidity drops, mist the inside of the container or add a small tray of water to help raise it. However, avoid over-saturating the environment, as stagnant moisture can lead to issues like mold or rot.
For larger growing areas, combining a humidifier with a misting routine can provide the best results. Set the humidifier to maintain the lower end of the humidity range (around 85%) and supplement with misting as needed, especially during drier periods or in low-humidity climates. Regularly monitor the environment, as factors like temperature, airflow, and the substrate’s moisture content can influence humidity levels. Adjust your methods accordingly to ensure the stem butt remains in an optimal humid environment.
Lastly, consider the substrate’s moisture content in conjunction with humidity management. While the air needs to be humid, the substrate should be moist but not waterlogged. Overwatering the substrate can reduce oxygen availability to the mycelium, while under-watering can cause it to dry out. Maintain a balance by checking the substrate’s moisture level regularly and misting or watering lightly as needed. By keeping both the air and substrate properly hydrated, you create an ideal environment for the stem butt to grow and produce a new flush of mushrooms.
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Control Temperature: Maintain 65-75°F (18-24°C) to encourage mycelium development and fruiting
Temperature control is a critical factor in successfully growing mushrooms from stem butts, as it directly influences mycelium development and fruiting. Mushrooms thrive in specific temperature ranges, and maintaining 65-75°F (18-24°C) creates an optimal environment for the mycelium to colonize the substrate and initiate fruiting. At this range, the mycelium remains active and healthy, ensuring robust growth and higher yields. Temperatures below 65°F (18°C) can slow down mycelium activity, delaying colonization and fruiting, while temperatures above 75°F (24°C) may stress the mycelium or encourage the growth of contaminants.
To maintain the ideal temperature range, start by selecting a suitable growing location. Basements, closets, or rooms with stable temperatures are excellent choices. Avoid areas prone to temperature fluctuations, such as near windows, heaters, or air conditioners. If natural conditions are not sufficient, consider using a thermostat-controlled environment, such as a grow tent or a small incubator, to ensure consistent temperatures. Monitoring tools like digital thermometers or temperature sensors can help you track the environment and make adjustments as needed.
During the colonization phase, when the mycelium is spreading through the substrate, maintaining the lower end of the temperature range (around 65-70°F or 18-21°C) can promote faster and more efficient growth. Once the substrate is fully colonized and you’re ready to induce fruiting, slightly increasing the temperature to the higher end of the range (70-75°F or 21-24°C) can encourage pinhead formation and fruiting body development. This slight adjustment mimics the natural conditions mushrooms experience in the wild, signaling to the mycelium that it’s time to produce fruit.
Humidity and ventilation also play a role in temperature control. High humidity levels, typically around 85-95%, are necessary for fruiting, but poor ventilation can lead to heat buildup, causing temperatures to rise above the optimal range. Ensure proper air exchange by using fans or passive ventilation methods while maintaining humidity with misting or a humidifier. Balancing these factors will help keep temperatures stable and conducive to mushroom growth.
Finally, be mindful of external factors that can affect temperature, such as seasonal changes or the heat generated by grow lights. If using artificial lighting, ensure it doesn’t raise the ambient temperature beyond the desired range. In colder climates, insulation or heating pads may be necessary to keep temperatures from dropping too low. By carefully managing temperature alongside other environmental factors, you can create the ideal conditions for growing mushrooms from stem butts, maximizing your chances of a successful harvest.
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Harvest and Repeat: Pick mature mushrooms, rehydrate the butt, and restart the cycle for multiple yields
Harvesting mushrooms from a stem butt is a sustainable and efficient way to grow multiple yields from a single source. Once your mushrooms have reached maturity, typically when the caps have fully opened and the gills are visible, it’s time to harvest. Use a sharp knife or your fingers to gently twist and pull the mushrooms from the butt, ensuring you don’t damage the remaining mycelium. Harvesting at the right time is crucial, as overripe mushrooms may release spores that could contaminate the growing area or exhaust the butt prematurely. After harvesting, clean the butt by removing any leftover mushroom fragments or debris to prevent contamination and prepare it for the next cycle.
Rehydrating the stem butt is the next critical step to restart the growing cycle. After harvesting, the butt will likely have dried out, so it needs moisture to reactivate the mycelium. Soak the butt in clean, non-chlorinated water for 12 to 24 hours, ensuring it’s fully submerged. Chlorinated water can harm the mycelium, so consider using filtered or distilled water. After soaking, drain the excess water and place the butt back into your growing container, which should be a humid, dark environment like a plastic box or bag with a substrate such as straw or wood chips. Maintain proper humidity levels by misting the container or using a humidifier to encourage new growth.
Once the butt is rehydrated and placed in the growing environment, monitor it closely for signs of new mushroom pins emerging. This process can take anywhere from a few days to a couple of weeks, depending on the mushroom species and environmental conditions. Keep the substrate and butt moist but not waterlogged, as excess moisture can lead to mold or bacterial growth. Temperature and humidity are key factors; most mushrooms thrive in temperatures between 60°F and 75°F (15°C and 24°C) with humidity levels around 80-90%. Adjust these conditions as needed to support healthy growth.
The ability to repeat this cycle multiple times is one of the greatest advantages of growing mushrooms from a stem butt. With proper care, a single butt can produce mushrooms for several cycles, though yields may decrease over time. After each harvest, inspect the butt for signs of degradation or contamination. If the butt becomes discolored, slimy, or emits a foul odor, it’s likely contaminated and should be discarded. Healthy butts will remain firm and white or slightly brown, indicating active mycelium. By consistently rehydrating and maintaining optimal conditions, you can maximize the productivity of the butt and enjoy a steady supply of fresh mushrooms.
To ensure long-term success, keep detailed records of each cycle, including harvesting times, rehydration methods, and environmental conditions. This information will help you refine your technique and troubleshoot any issues. Additionally, consider experimenting with different substrates or environmental adjustments to optimize yields. Growing mushrooms from a stem butt is not only a cost-effective method but also an eco-friendly way to cultivate your own food. With patience and attention to detail, you can master the harvest-and-repeat process and enjoy multiple bountiful yields from a single stem butt.
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Frequently asked questions
The stem butt method involves using the base of a mushroom stem (the part that connects to the cap) to grow new mushrooms. This method is particularly effective for certain species like oyster mushrooms.
Clean the stem butt gently with a damp cloth to remove any dirt or debris. Allow it to dry slightly, then place it on a damp paper towel or directly into a growing medium like straw, wood chips, or coffee grounds.
Mushrooms thrive in a humid environment with temperatures between 65–75°F (18–24°C). Keep the growing medium moist but not waterlogged, and ensure good air circulation. Indirect light is sufficient, as mushrooms do not require sunlight.
It typically takes 2–4 weeks for mycelium (the mushroom's root system) to colonize the growing medium, and another 1–2 weeks for mushrooms to form. Patience is key, as growth times can vary based on conditions.
Yes, some stem butts can be reused for a second or even third flush of mushrooms. After harvesting, rehydrate the stem butt and replant it in fresh or rehydrated growing medium, maintaining optimal conditions for continued growth.

























