
Growing reishi mushrooms (Ganoderma lucidum) from plugs is a rewarding and accessible way to cultivate these medicinal fungi at home. Reishi mushrooms are prized for their immune-boosting and stress-relieving properties, making them a popular choice for both hobbyists and health enthusiasts. The process begins by selecting high-quality reishi mushroom plugs, which are small wooden dowels inoculated with mycelium, the vegetative part of the fungus. These plugs are then inserted into a suitable substrate, such as hardwood logs or stumps, creating an ideal environment for the mycelium to colonize and eventually produce fruiting bodies. Proper preparation of the substrate, including drilling holes and sealing the plugs with wax, is crucial for success. With patience and the right conditions—such as shade, humidity, and adequate moisture—reishi mushrooms can thrive, offering a sustainable source of this valuable fungus for personal use or small-scale production.
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What You'll Learn
- Prepare substrate: Sterilize hardwood sawdust or straw, mix with nutrients, and pack into grow bags
- Inoculate plugs: Insert reishi mycelium plugs into pre-drilled holes in logs or substrate
- Maintain humidity: Keep environment at 60-70% humidity using misting or humidifiers for optimal growth
- Monitor temperature: Ensure 70-75°F (21-24°C) for healthy mycelium colonization and fruiting
- Harvest reishi: Cut mature mushrooms at the base when caps are fully developed but firm

Prepare substrate: Sterilize hardwood sawdust or straw, mix with nutrients, and pack into grow bags
To prepare the substrate for growing reishi mushrooms from plugs, the first step is to sterilize the hardwood sawdust or straw. This is crucial to eliminate any competing organisms that could hinder the growth of your reishi mushrooms. Begin by sourcing high-quality hardwood sawdust or straw, ensuring it is free from contaminants. Place the sawdust or straw in a large pot or pressure cooker. If using a pot, add water to cover the material and bring it to a boil for at least 1 hour. For a pressure cooker, follow the manufacturer's instructions, typically sterilizing at 15 PSI for 1.5 to 2 hours. Proper sterilization ensures a clean environment for the reishi mycelium to thrive.
After sterilizing, allow the sawdust or straw to cool to a temperature that is safe to handle, usually around 80-90°F (27-32°C). While cooling, prepare the nutrient supplement, which typically includes a mixture of wheat bran, gypsum, and other organic nutrients. The general ratio is 5-10% supplement to the weight of the sawdust or straw. Mix the cooled, sterilized substrate with the nutrient supplement thoroughly, ensuring an even distribution. This step is vital as it provides the necessary nutrients for the reishi mycelium to grow and develop into healthy mushrooms.
Once the substrate is mixed with nutrients, it’s time to pack it into grow bags. Use autoclavable polypropylene grow bags with a filter patch to allow for gas exchange while keeping contaminants out. Fill each bag to about ¾ full, lightly packing the substrate to remove air pockets but avoiding compaction, as the mycelium needs oxygen to grow. Seal the bags loosely with a fold or use a bag sealer, ensuring they are secure but not airtight. Properly packed bags create an optimal environment for the reishi plugs to colonize the substrate.
Before introducing the reishi plugs, it’s essential to ensure the substrate is fully sterilized and cooled. Any residual heat or contamination can jeopardize the entire process. After packing, let the bags sit for a day to stabilize and cool completely. This waiting period allows any remaining moisture to distribute evenly and ensures the substrate is ready for inoculation. With the substrate prepared, you’re now set to insert the reishi mushroom plugs and begin the colonization process.
Finally, label each grow bag with the date and type of substrate used for easy tracking. Store the bags in a clean, dark environment with stable temperatures between 70-75°F (21-24°C). This setup encourages the reishi mycelium to spread efficiently through the substrate. Regularly inspect the bags for any signs of contamination, such as mold or unusual colors, and remove any affected bags immediately to protect the rest of your crop. With careful preparation and attention to detail, your substrate will provide the perfect foundation for growing robust reishi mushrooms.
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Inoculate plugs: Insert reishi mycelium plugs into pre-drilled holes in logs or substrate
Inoculating plugs is a critical step in growing reishi mushrooms from plugs, as it involves introducing the reishi mycelium into a suitable substrate where it can colonize and eventually produce mushrooms. Begin by preparing your logs or substrate, which should be hardwood such as oak, maple, or beech, as reishi mushrooms thrive on these types of wood. Ensure the logs are freshly cut, ideally within a few weeks, to provide the mycelium with the maximum nutrients. Drill holes into the logs using a 5/16-inch drill bit, spacing them about 6 inches apart in a diamond pattern to allow for adequate mycelial growth. The holes should be slightly smaller than the diameter of the reishi mycelium plugs to ensure a snug fit, which helps prevent contamination and promotes better colonization.
Once the holes are drilled, it’s time to insert the reishi mycelium plugs. Gently tap each plug into a pre-drilled hole using a rubber mallet or a similar tool, ensuring the plug is inserted firmly but not forced, as this could damage the mycelium. The goal is to have the plug flush with the surface of the log or slightly below it. After inserting all the plugs, seal the holes with wax to protect the mycelium from drying out and to deter competing fungi or insects. Use cheese wax or a specialized inoculation wax, melting it carefully and applying it over the plugs while the wax is still warm but not hot enough to damage the mycelium.
If you’re using a substrate other than logs, such as sawdust or straw blocks, the process is similar but requires a different approach. Prepare the substrate by sterilizing it to eliminate any competing organisms, then allow it to cool before introducing the reishi mycelium plugs. Create small holes in the substrate using a tool like a dowel or a specialized substrate inoculator, ensuring the holes are the right size for the plugs. Insert the plugs into these holes, pressing them gently to ensure good contact between the mycelium and the substrate. Cover the inoculation points with a thin layer of sterile substrate or wax to maintain humidity and protect the mycelium.
After inoculation, the logs or substrate must be placed in an environment conducive to mycelial growth. Keep them in a shaded, humid area, such as a forest or a purpose-built structure, where temperatures range between 60°F and 80°F (15°C and 27°C). Mist the logs or substrate periodically to maintain moisture levels, but avoid overwatering, as excessive moisture can lead to contamination. The mycelium will begin colonizing the substrate, a process that can take several months, depending on environmental conditions and the health of the mycelium.
Patience is key during the colonization phase, as rushing the process can lead to poor results. Monitor the logs or substrate regularly for signs of contamination, such as mold or unusual colors, and address any issues promptly. Once the mycelium has fully colonized the substrate, typically indicated by white mycelial growth visible through the bark or substrate surface, the reishi mushrooms will begin to fruit. This stage requires slightly different conditions, including increased airflow and specific humidity levels, to encourage mushroom development. With proper care and attention during the inoculation and colonization stages, you’ll be well on your way to harvesting your own reishi mushrooms.
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Maintain humidity: Keep environment at 60-70% humidity using misting or humidifiers for optimal growth
Maintaining the correct humidity level is crucial for successfully growing reishi mushrooms from plugs, as these fungi thrive in environments with consistent moisture. The ideal humidity range for reishi cultivation is between 60% and 70%, which mimics their natural habitat and promotes healthy growth. To achieve this, you can employ misting techniques or use humidifiers, depending on your setup and resources. Misting involves spraying a fine layer of water around the growing area, which increases humidity without saturating the substrate. Use a spray bottle with a gentle mist setting, and aim to mist the walls of the growing container or the surrounding air rather than directly soaking the plugs or substrate. This should be done 2–3 times daily, especially during the initial stages when the mycelium is colonizing.
If misting is not sufficient or practical, investing in a humidifier is a reliable alternative. Humidifiers provide a steady and controlled moisture level, ensuring the environment remains within the optimal range. Place the humidifier near the growing container or fruiting chamber, and monitor the humidity using a hygrometer to make adjustments as needed. For reishi mushrooms, ultrasonic or evaporative humidifiers work well, as they produce a fine mist that raises humidity without creating excessive condensation, which can lead to mold or bacterial issues. Ensure the humidifier is cleaned regularly to prevent the buildup of minerals or bacteria that could harm the mushrooms.
In addition to misting or using a humidifier, proper ventilation is essential to maintain humidity without causing waterlogged conditions. Ensure your growing area has adequate airflow by using vents or fans, but avoid directing airflow straight at the mushrooms, as this can dry them out. A balanced approach to humidity and ventilation will prevent stagnant air while keeping moisture levels stable. If you notice condensation forming on the walls of the growing container, reduce misting frequency or adjust the humidifier settings to avoid excess moisture, which can suffocate the mycelium or encourage contaminants.
For growers using a fruiting chamber, such as a grow tent or plastic enclosure, lining the walls with a moisture-retaining material like perlite or vermiculite can help stabilize humidity. These materials absorb excess moisture when humidity is high and release it when levels drop, creating a more consistent environment. Pairing this with a humidifier or regular misting ensures the reishi mushrooms receive the moisture they need without fluctuations that could stress the mycelium. Regularly monitor the humidity levels, especially during the fruiting stage, as reishi mushrooms are particularly sensitive to dryness during this period.
Lastly, consider the ambient conditions of your growing space, as external factors like heating or air conditioning can impact humidity. If your growing area is prone to dryness, you may need to mist more frequently or use a larger humidifier. Conversely, in naturally humid environments, you might rely less on artificial humidification but still need to monitor levels to prevent oversaturation. By maintaining a consistent humidity range of 60–70%, you create an environment where reishi mushroom plugs can thrive, leading to robust mycelium growth and healthy fruiting bodies.
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Monitor temperature: Ensure 70-75°F (21-24°C) for healthy mycelium colonization and fruiting
Temperature control is a critical factor in successfully growing reishi mushrooms from plugs, as it directly influences the growth rate and overall health of the mycelium. Reishi mushrooms thrive in a specific temperature range, and maintaining this range is essential for both mycelium colonization and fruiting. The ideal temperature for reishi cultivation is between 70-75°F (21-24°C). At this range, the mycelium can efficiently break down the substrate, absorb nutrients, and prepare for fruiting. If the temperature falls below or exceeds this range, the growth process may slow down or even halt, leading to poor yields or contaminated crops.
To monitor temperature effectively, invest in a reliable thermometer or a digital temperature sensor that can provide accurate and continuous readings. Place the sensor inside the growing environment, such as a grow tent, greenhouse, or even a simple plastic container, to ensure it reflects the actual conditions experienced by the mushroom plugs. Regularly check the temperature throughout the day, as fluctuations can occur due to external factors like room heating, cooling systems, or natural temperature changes. If you notice the temperature drifting outside the optimal range, take immediate steps to adjust the environment, such as using heaters, fans, or insulation to stabilize the conditions.
Maintaining consistent humidity levels alongside the correct temperature is also crucial, as these two factors work together to create an ideal growing environment. However, the focus here is on temperature, and it’s important to avoid the common mistake of overheating or overcooling the space. For instance, while reishi mushrooms can tolerate slightly cooler temperatures during colonization, fruiting requires a more stable and warmer environment within the specified range. Using a thermostat-controlled heating or cooling system can help automate temperature regulation, ensuring that the mycelium remains in its optimal zone without constant manual intervention.
For growers using indoor setups, such as grow tents or cabinets, monitoring temperature becomes even more manageable with the right equipment. Consider using a temperature controller that can activate heating or cooling devices as needed to maintain the 70-75°F range. Additionally, placing the growing container away from windows, doors, or vents can prevent sudden temperature shifts. If you’re growing reishi in a natural environment, like a shed or outdoor space, monitoring becomes more challenging but equally important. Use shade cloths, insulation, or even buried containers to buffer against extreme outdoor temperatures and keep the internal environment stable.
Finally, observe the mycelium’s response to temperature changes as a visual indicator of whether conditions are optimal. Healthy mycelium should appear white, fluffy, and actively spreading throughout the substrate. If the mycelium turns brown, slows in growth, or shows signs of contamination, it may indicate that the temperature is not within the ideal range. By consistently monitoring and adjusting the temperature, you create a stable environment that encourages robust mycelium colonization and, ultimately, successful fruiting of reishi mushrooms. This attention to detail will significantly improve your chances of a bountiful harvest.
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Harvest reishi: Cut mature mushrooms at the base when caps are fully developed but firm
Harvesting reishi mushrooms at the right time is crucial to ensure optimal quality and potency. The key indicator for harvesting is the maturity of the mushroom caps. When the caps are fully developed but still firm to the touch, it’s time to harvest. Overripe reishi mushrooms may become woody and less desirable, so timing is essential. To determine readiness, observe the caps: they should have a vibrant color, typically a deep reddish-brown, and a smooth, glossy surface. The edges of the cap should be slightly curled downward, and the overall structure should feel solid yet yield slightly when pressed.
To harvest reishi mushrooms, use a clean, sharp knife or pruning shears to cut the mushroom at its base. This method ensures minimal damage to the growing substrate and allows the mycelium to continue producing more mushrooms in the future. Hold the mushroom firmly but gently to avoid tearing the tissue, and make a clean, precise cut just above the substrate or wood surface. Avoid pulling or twisting the mushroom, as this can damage the mycelium and reduce future yields. Each cut should be swift and deliberate to maintain the integrity of both the harvested mushroom and the remaining growth.
After cutting, inspect the base of the mushroom to ensure no part of it remains attached to the substrate, as this can lead to decay or contamination. Properly harvested reishi mushrooms should have a clean, smooth stem end. If there are any signs of mold, pests, or disease on the mushroom or the substrate, address these issues immediately to protect the rest of your crop. Healthy mushrooms will have a fresh, earthy aroma and a firm texture, indicating they are ready for drying or processing.
Once harvested, reishi mushrooms should be handled with care to preserve their medicinal properties. Place them in a well-ventilated area away from direct sunlight to begin the drying process. Drying is essential to extend their shelf life and concentrate their bioactive compounds. Properly dried reishi mushrooms will become lightweight and brittle, making them ideal for grinding into powder or brewing into tea. Harvesting at the right stage and handling the mushrooms correctly ensures you get the most out of your reishi cultivation efforts.
Finally, after harvesting, monitor the substrate for signs of new growth. Reishi mushrooms often produce multiple flushes, especially if the growing conditions remain favorable. Keep the substrate moist and maintain proper humidity and temperature levels to encourage additional fruiting. By following these harvesting techniques and post-harvest care steps, you can maximize the yield and quality of your reishi mushrooms grown from plugs, ensuring a successful and rewarding cultivation experience.
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Frequently asked questions
Reishi mushrooms thrive in a humid environment with temperatures between 65–75°F (18–24°C). They require indirect light and good air circulation. Use a substrate like hardwood logs or sawdust supplemented with nutrients, and maintain moisture by misting regularly or using a humidifier.
Reishi mushrooms are slow-growing and can take 6–12 months to start fruiting after inoculating plugs into the substrate. Full maturity and harvest may take 1–2 years, depending on environmental conditions and care.
Yes, reishi mushrooms can be grown indoors using a controlled environment. Use a grow tent or shelf with proper humidity, temperature, and airflow. Inoculate plugs into supplemented sawdust bags or hardwood blocks, and ensure consistent moisture for successful growth.

























