Printing Mushroom Spores: A Step-By-Step Guide

how to print mushroom spores

Mushroom spores are like seeds, carrying half the genetic information needed to produce a mushroom. They are released into the environment from the gills or pores under the cap of a mature mushroom and are carried away by air currents. Spore printing is a simple process that allows you to see the colour and pattern of spores, which is useful for identifying mushrooms. To make a spore print, cut off the stem of a mushroom and place the cap, gill-side down, on a piece of paper, foil, or glass. Cover it with a bowl or cup to protect it from air currents and leave it for a few hours or overnight. The spores will fall and settle, creating a spore print pattern. Spore prints can be preserved with hair spray or artist fixative and even framed as artwork.

Characteristics Values
Purpose Identification of mushroom species
Mushroom Types Gills, pores, morels, hydnums, corals, puffballs, bird's nests
Mushroom Characteristics Mature, soft, fresh, pale/dark gills
Materials Mushroom cap, paper (black/white), tinfoil, glass, scalpel/sharp knife, water, glass/bowl/paper cup, artist's fixative/hairspray
Process Cut the stem, place the cap on the material with gills facing down, add a drop of water, cover with glass/bowl, leave for 2-24 hours, spray with fixative/hairspray
Preservation Store in a ziploc bag at room temperature

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Choosing the right mushroom

When choosing the right mushroom for spore printing, there are a few key considerations to keep in mind. Firstly, it is important to select a mature mushroom as spores are not produced until near the end of the mushroom's life cycle. Young mushrooms may even have a "veil" covering the gills, so choosing a mushroom with fully open and exposed gills is ideal. Portobello mushrooms, for example, typically have open caps and well-defined gills, making them a good choice.

The colour of the mushroom's gills can also guide your selection. If you are using white paper for printing, choose mushrooms with darker gills, such as portobellos, as their spores will show up better. Conversely, if you opt for black paper, mushrooms with pale gills are more suitable. Shiitake mushrooms, for instance, have white gills that create light spore prints, so they are best printed on black paper.

Additionally, consider the surface on which you will be making the spore print. If you plan to print on paper, foil, or glass, certain mushrooms may be more conducive. For instance, shelf mushrooms and oyster-type mushrooms can sometimes be used whole, making them a versatile choice for various surfaces. If you intend to print on glass, the dreamy effect of the spores drifting down can be quite captivating.

It is worth noting that some mushrooms produce both dark and light spore prints, so if you are specifically aiming for a particular colour, consulting a mushroom guide or identification guide can be helpful. These guides often provide information on the spore print colour of different mushroom species. Additionally, if you are planning to use the spores for cultivation, choosing a print surface that can be easily sterilised, such as tinfoil, is recommended.

Lastly, when choosing the right mushroom, it is important to follow ethical and sustainable practices. Only pick mushrooms if there are plenty around, as they play a crucial role in the environment as decomposers. Remember to wash your hands after handling mushrooms and always collect them in a basket or paper bag to avoid damage.

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Preparing the mushroom

Firstly, choose a mature mushroom that is mature in age, as spores are not produced until near the end of a mushroom's life cycle. Look for a mushroom with visible gills, such as oyster, portobello, or Swiss brown mushrooms. If you are collecting mushrooms from the wild, remember to follow local guidelines and only pick a mushroom if there are plenty available, as they play an important ecological role.

Once you have selected your mushroom, carefully remove the cap from the stem. Cut off the stem as close to the gills as possible to ensure that the cap can sit flat. This step may vary slightly depending on the type of mushroom. For example, if you have a shelf mushroom or an oyster-type mushroom, you might be able to use the whole mushroom for printing.

After removing the cap, place it gill-side down onto a piece of paper, foil, or glass. The colour of the surface you choose will depend on the colour of the spores. White paper is suitable for mushrooms with darker gills, while black paper works well for mushrooms with pale gills. If you are using the spore print for identification purposes and intend to examine the spores under a microscope, it is recommended to use a microscope slide or a slide cover slip. Additionally, if you plan on growing mushrooms from the spores, tin foil is preferred as it is more sterile and easier to work with.

To enhance spore release, place a drop of water on top of the cap. Then, cover the cap with a glass, bowl, tumbler, or paper cup to prevent it from drying out and to protect it from air currents that might carry away the spores. For smaller mushrooms that tend to dry out quickly, place a piece of wet tissue paper on top of the cap before covering it.

Now that your mushroom is prepared, leave it undisturbed for 2 to 24 hours, depending on the humidity and freshness of the mushroom. During this time, the spores will fall and create a beautiful spore print pattern.

Remember to practise caution when handling mushrooms, and always wash your hands after touching them.

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Selecting the surface

When selecting a surface to print mushroom spores, there are several options to choose from, each with its own advantages and considerations. Here are some detailed instructions and guidelines for selecting the appropriate surface:

Paper:

Using paper as a surface for spore printing is a common and simple method. A normal sheet of white paper is typically sufficient for most mushrooms. However, it is important to consider the colour of the spores and choose the paper colour accordingly. For mushrooms with darker-coloured gills, opt for white paper, whereas for mushrooms with pale gills or white spores, black paper can provide better visibility. If you plan to use the spore print for identification purposes, having the spores stand out against the paper colour is crucial. Additionally, if you intend to observe the spores under a microscope, using a microscope slide or a slide cover slip is ideal.

Aluminum Foil:

Aluminum foil is another suitable surface for collecting mushroom spores. It is versatile and can accommodate any colour of spores. Foil is also more sterile than paper, as it can be wiped down with alcohol, making it a preferred choice if you intend to grow mushrooms from the spores or use them for cultivation. Additionally, spore prints on foil can be easily preserved by spraying them with an artist's fixative or hair spray.

Glass:

Using a piece of glass, such as a glass microscope slide or a small square of glass, can provide an interesting effect for spore printing. Glass is suitable for all spore colours and can be useful for observing the spores under a microscope. Similar to foil, glass can be sterilised, making it a good option for cultivation purposes. To preserve the spore print, you can simply cover it with a glass tumbler or bowl to protect it from external elements.

Other Surfaces:

While less commonly mentioned, there are other surfaces that can be used for spore printing. Card stock or coloured paper can be used for artistic purposes, allowing the spores to "float" down and create unique patterns due to air currents. Additionally, clear plastic can be utilised for mushrooms with white spores, providing a similar effect to glass.

In summary, the selection of the surface depends on the intended purpose of your spore print. For identification, choose a surface that provides good contrast with the spore colour. For artistic purposes, feel free to experiment with different surfaces to create unique and visually appealing spore prints. For cultivation or growing mushrooms, opt for surfaces that can be sterilised, such as foil or glass.

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Covering the mushroom

When preparing to print mushroom spores, it is important to cover the mushroom cap with a suitable container to prevent the spores from being dispersed by air currents. Here are some detailed instructions on how to cover the mushroom effectively:

Choosing the Right Container

Use a glass, bowl, or another suitable container to cover the mushroom cap. A glass tumbler or a small bowl is recommended to create a controlled environment. If you are using a glass, ensure it is large enough to cover the entire cap without touching the surface. Alternatively, you can use a paper cup, especially if you are concerned about the mushroom getting soft.

Creating a Controlled Environment

Place the chosen container over the mushroom cap to prevent air currents from carrying away the spores. Make sure the cap is centred and in full contact with the surface to ensure a clear and accurate print. If you are using a bowl, you can place a piece of wet tissue paper on top of the cap to prevent it from drying out.

Timing and Checking

Leave the mushroom covered for a few hours or overnight. The exact duration may vary depending on the freshness of the mushroom and the humidity of the environment. It is recommended to check periodically to ensure the spore print has fully developed. Do not leave the mushroom covered for more than 24 hours, as older specimens may start to rot or attract larvae.

Preserving the Spore Print

Once you have achieved a clear spore print, you can preserve it by covering it with a transparent sheet, such as plastic wrap. Alternatively, you can use an artist spray, hair spray, or lacquer to fix the spores onto the paper. If you plan on taking multiple spore prints, consider laminating the paper so that you can wipe it clean and reuse it.

Storage and Handling

After removing the container, carefully lift the mushroom cap to avoid smudging the spore print. If you intend to store the spore prints, fold the paper or foil and place them in a ziplock bag. Spore prints can be stored at room temperature and can last for decades.

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Storing the spore print

Refrigeration

One popular method for storing spore prints is to place them in a refrigerator. Spore prints should be stored in a sealed bag or airtight container in the fridge. Maintaining a consistent temperature between 2°C and 8°C (35°F and 46°F) is crucial. Avoid freezing the spore prints, as this can damage them and reduce their viability. Under optimal conditions, spore prints can remain viable for a year or even longer.

Room Temperature Storage

Alternatively, you can store spore prints at room temperature. It is recommended to keep them in a dark place, away from direct sunlight, such as a cabinet, closet, or drawer. Avoid locations with significant temperature fluctuations, such as rooms with exterior walls or windows. Spore prints stored at room temperature can remain viable for about six months.

Long-Term Storage

For long-term storage, spore prints can be sealed in their original packaging or airtight containers and placed in the freezer. While freezing may extend the viability of the spore prints, there is a risk of damage due to freezing temperatures. This method is not commonly recommended for mushroom cultivation purposes.

Display and Storage

If you wish to display your spore prints while also keeping them protected, consider using a binder or scrapbook. Place the spore print in a protective case, such as a plastic sleeve or UV-protected plastic, and then slide it into a binder sleeve or scrapbook page. This allows you to showcase your collection while keeping the prints safe from contamination.

Storage Containers

When storing spore prints, it is important to use clean and sterile containers. Wipe down the packaging or containers with 70% isopropyl alcohol before placing the spore prints inside. Ensure that the containers are airtight to prevent contamination and maintain the viability of the spore prints over time.

By following these storage instructions, you can effectively preserve your spore prints and ensure their longevity for successful mushroom cultivation.

Frequently asked questions

You will need a mature mushroom, a sheet of paper, foil, or glass, a scalpel or sharp knife, a glass or bowl, and a ziploc bag for storage.

First, cut off the mushroom stem so that the cap can sit flat on the paper, foil, or glass. Place the cap gill-side down and cover it with a glass or bowl to prevent spores from being carried away by air currents. Leave it for 2-12 hours, then remove the cap. You should have a fully formed mushroom spore print.

Fold over the paper or foil containing the spore print and store it in a ziploc bag at room temperature. Spore prints can last for decades and do not need to be refrigerated, even if you plan to use them for cultivation. You can also preserve spore prints by spraying them with an artist's fixative or hairspray.

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