
Mushrooms are fascinating organisms that play a crucial role in ecosystems. They are a type of fungus, with a unique reproductive mechanism that involves the production and release of spores. These spores, akin to seeds, are microscopic and can give rise to new mushrooms under the right conditions. The process of spore release varies depending on the type of fungus, but it typically involves the maturation of the fungus and the subsequent discharge of spores. One intriguing aspect of mushrooms is that they can leave behind spore prints, which are not only aesthetically pleasing but also serve as a means of identification. By understanding the characteristics of these prints, including their colour, shape, texture, and pattern, mycologists can identify different species of mushrooms without relying on high-powered microscopes. Creating spore prints is a relatively simple process that involves selecting a mushroom with visible gills, placing it gill-side down on paper, and covering it gently to maintain humidity. The spores will then settle onto the paper, creating intricate and symmetrical patterns that offer a glimpse into the hidden world of fungi.
| Characteristics | Values |
|---|---|
| Mushroom type | Any edible mushroom with gills, such as oyster, portobello, Swiss brown, or shiitake |
| Mushroom colour | Dark-gilled mushrooms like portobellos have dark spores, whereas shiitake mushrooms have white gills and spores |
| Paper colour | White paper for dark-gilled mushrooms, black paper for pale-gilled mushrooms |
| Mushroom preparation | Cut off the stipe/stem so that the cap can sit flat, then place gill/spore side down on the paper |
| Equipment | Glass or bowl to cover the mushroom and paper, fixative or hairspray (optional) |
| Timing | Leave covered overnight, then uncover carefully the next day |
| Result | A spore print, which can be used for art or to identify the mushroom species |
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What You'll Learn

Choosing the right mushroom
Maturity
Mushrooms need to be mature enough to produce spores. Look for mushrooms with fully opened caps and exposed gills, pores, or spines. The gills should be free of any "veil" that may be protecting them during the early stages of development.
Accessibility of Spore-bearing Structures
The spores are typically found on the undersides of the caps, so choose a mushroom that allows easy access to this area. This accessibility will make it more convenient when you place the cap gill-side down for spore printing.
Spore Color
While spore color alone should not be the only factor for identification, it can be a helpful characteristic. Different species of mushrooms have spores in various colors, ranging from white, purple, rusty-brown, orange-brown, cream, pink, chocolate brown, to black. If you're unsure about the expected spore color, consider making spore prints on different colored papers, such as black and white, to capture the contrast effectively.
Freshness and Humidity
The freshness and humidity of the mushroom will impact the time needed for spore printing. Fresher mushrooms may require less time for the spores to fall and create a print.
Type of Mushroom
Some mushrooms, such as shelf mushrooms and oyster-type mushrooms, may be easier to work with for spore printing. They may not require removing the cap and can be used whole. Additionally, consider the purpose of your spore printing. If you plan to grow mushrooms from spores, using tinfoil is recommended as it is more sterile and suitable for making spore syringes.
Remember, when collecting mushrooms, always follow local regulations and respect private property and protected areas. Wash your hands after handling mushrooms, and only pick them if there are plenty in the area, as they play an important role in the environment.
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Preparing the mushroom
Selecting the Mushroom: Choose a fresh, mature mushroom with visible gills. Common varieties such as oyster, portobello, shiitake, and Swiss brown mushrooms are good options. You can opt for store-bought mushrooms or forage for them in your local ecosystem. Ensure the gills are apparent, as these are the structures that house the spores.
Cutting the Stipe: Using a sharp knife or scissors, carefully cut off the stipe (stem) of the mushroom. Make sure to cut as close to the gills as possible so that the cap can sit flat on the surface. This step ensures that the spores can be released effectively.
Placing the Cap: Once the stipe is removed, gently place the mushroom cap, gill-side down, onto a piece of paper. The colour of the paper depends on the type of mushroom you're using. For mushrooms with pale gills or light-coloured spores, use black paper. For mushrooms with darker gills or spores, opt for white paper. This contrast ensures that the spores are visible against the background.
Covering the Mushroom: To maintain humidity and prevent the mushroom from drying out, cover it gently with a glass container, bowl, or upside-down glass. This creates a microenvironment that mimics the natural conditions in which spores are released. It also protects the spores from external disturbances.
Leaving it Overnight: Leave the covered mushroom undisturbed overnight. During this time, the spores will naturally drift down and settle, creating a delicate spore print. The length of time required may vary, but generally, leaving it overnight is sufficient for the spores to drop onto the paper.
Revealing the Spore Print: In the morning, carefully remove the glass or bowl and lift the mushroom cap. You should now see the intricate spore print revealed on the paper. The print may appear as a fine, delicate dust, forming a symmetrical pattern that traces the gills' structure.
By following these steps, you can effectively prepare your mushroom and create the optimal conditions for spore release, resulting in a beautiful and informative spore print.
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Paper and colour
When creating a spore print, it is recommended to use white and black paper side by side. White paper is suitable for mushrooms with non-white spore prints, while black paper is ideal for capturing white or light-coloured spores. If you already know the colour of the spores, you can choose a coloured paper that will highlight the spore colour. For example, if you know that the mushroom drops white spores, a black piece of paper will make them stand out.
Laminating the black and white paper together is a useful tip, especially if you plan on taking multiple spore prints. This way, you can wipe the laminated paper clean and reuse it. If you are planning to use the spore print for cultivation purposes, it is important to maintain a sterile environment and clean your workspace, hands, and tools with isopropyl alcohol or a disinfectant.
Mushroom spores come in a variety of colours, including white, cream, black, shades of red, purple, brown, and green. The colour of the spores can be observed by removing the mushroom cap after a few hours or overnight. The imprint on the paper will not only show the colour but also patterns corresponding to the lamellae or pores of the fungus.
Spore prints can be used for artistic purposes as well. By allowing the spores to "float" down onto the paper in patterns created by air currents, you can create unique designs. Spore prints can be preserved and displayed by covering them with lacquer or hair spray, adding interesting designs, and getting creative.
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Covering the mushroom
Choosing a Cover
To prevent air currents from carrying away the spores, it is essential to use a cover that fits snugly over the mushroom cap. Common household items that can be used as covers include glass cups or bowls, paper cups, or small containers. The cover should be large enough to enclose the mushroom cap completely but not so large that it disturbs the arrangement.
Placement of the Cover
Gently place the chosen cover over the mushroom cap, ensuring that it is centred and in full contact with the surface. This step is crucial to prevent any gaps through which air currents can enter and disturb the spores. Take care not to touch the gills of the mushroom, as they are delicate and contain the spores you are trying to collect.
Preventing Air Dispersal
By covering the mushroom cap, you create a microenvironment that shields the spores from air currents. This ensures that the spores fall directly onto the desired surface, such as paper or foil, rather than being carried away. The cover also helps to maintain humidity, which can aid in the release and collection of spores.
Timing and Observation
After covering the mushroom cap, the waiting period begins. Leave the covered mushroom undisturbed for 6 to 12 hours or even overnight. The duration depends on the freshness of the mushroom and the humidity of the environment. It is a good idea to check periodically to ensure the spores have started to drop and form a print. Avoid leaving the mushroom covered for more than 24 hours, as older specimens may attract larvae or begin to rot.
Uncovering the Mushroom
Once the waiting period is over, carefully remove the cover by lifting it straight up and avoiding any contact with the spores. You should now see a spore print, which may reveal intricate patterns and colours. Take caution not to smudge or disturb the delicate spore print. If you are satisfied with the print, you can proceed to the next steps of preservation or identification.
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Harvesting spores
Gill mushrooms are the easiest to harvest spores from, but with some experimentation, spores can be harvested from all types of mushrooms. The most common method for harvesting spores from mushrooms is by making a spore print. Here is a step-by-step guide to creating a spore print:
Firstly, choose a mushroom with visible gills, such as an oyster, portobello, or Swiss brown mushroom. Ensure that the mushroom is mature, with gills readily apparent. The mushroom's gills contain billions of spores.
Next, cut off the stipe (stem) of the mushroom, right up to the gills, so that the cap can sit flat. Place the mushroom gill-side down on a piece of paper. The colour of the paper depends on the colour of the mushroom's gills. If the mushroom has pale gills, use black paper. If the gills are darker, use white paper. This is because the spores of mushrooms with darker gills, such as portobellos, tend to be darker, while mushrooms with pale gills, such as shiitakes, tend to have white spores. However, some mushrooms make both dark and light spore prints, so using two colours of paper side by side will ensure that the spores are visible regardless of their shade.
Cover the mushroom gently with a glass bowl or container to prevent it from drying out and to maintain humidity. Leave it overnight. During this time, the spores will drift down and settle, tracing out the pattern of the gills in fine, delicate dust.
In the morning, carefully lift the mushroom and the glass bowl or container. If the paper has absorbed a lot of moisture from the mushroom, it may need to dry before the spore print can be seen clearly. The spore print may be used as a work of art, or to identify the mushroom species. The colour, shape, texture, and pattern of the spores can be used to identify the mushroom without the need for a high-powered microscope.
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Frequently asked questions
Mushroom spores are like seeds, but they are structurally different. They are microscopic and are released by the mushroom when it matures.
A spore print is a method used to identify a fungus by examining the colour, shape, texture, and pattern of the released spores. It is usually made on a piece of paper or a glass plate.
To make a mushroom spore print, you need an edible mushroom with visible gills, such as an oyster, portobello, or Swiss brown mushroom. Cut off the stem so that the cap can sit flat, and place it gill-side down on a piece of paper. Cover it gently with a glass or bowl to protect it from drafts and increase humidity, and leave it overnight. In the morning, carefully lift the cap to reveal your spore print.
The colour of the paper depends on the colour of the spores. Mushrooms with dark spores, such as portobellos, show up well on white paper, while mushrooms with light-coloured spores, such as shiitakes, show up better on black paper.
























