
Contaminated mushrooms are those that have been invaded by unwanted microorganisms that interfere with their growth and cause long-term problems or
| Characteristics | Values |
|---|---|
| Types of Contamination | Bacteria, Mould (Fungi), Pests |
| Common Bacteria | Bacillus spp. (known as "wet spot" or "sour rot") |
| Appearance of Bacterial Contamination | Slimy, wet patches; dull gray, slimy, excessively wet, mucus-like |
| Appearance of Trichoderma (a common fungus) | Aggressive white mycelium that causes soft decay in mushrooms before turning a vibrant green |
| Appearance of Mould | Smooth, sponge-like; dense mycelium; fungus may appear as a dust or powder-like layer on the substrate |
| Appearance of Penicillium (most common type of mould) | Starts out white; colonies are usually circular; musty dirt smell |
| Appearance of Aspergillus (another common fungus) | Light gray, similar to mushroom mycelium; can also be yellow, black, green, brown, or blue |
| Signs of Contamination | Discolouration (green, blue, grey, black, yellow, brown), strange odour, slimy patches, crusty or gel-like textures |
| Prevention | Sterilization, pasteurization, substrate sterilization, air filtration, use of a laminar flow hood or still air box, sanitation, use of airtight grow room |
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What You'll Learn
- Bacteria, including Bacillus spp., can be identified by slime and treated with pasteurisation and sterilisation
- Fungi, including Trichoderma, can be identified by discolouration and treated with salt
- Pests, tiny insects that transmit disease, can be controlled with strict sanitation and an airtight grow room
- Human contamination, the greatest source of contamination, can be mitigated with hand washing, sanitiser, clean clothes, face masks, and gloves
- Airborne contamination, including mould spores, can be reduced with a laminar flow hood or still air box

Bacteria, including Bacillus spp., can be identified by slime and treated with pasteurisation and sterilisation
Mushrooms are vulnerable to contamination by bacteria, mould, and pests. Bacterial contamination is often identified by the presence of slime, as well as brown and yellow stains, and crusty or gel-like textures. The most common type of bacteria that contaminates mushrooms is Bacillus spp., also known as "wet spot" or "sour rot". This is a rod-shaped, Gram-positive, aerobic or (under certain conditions) anaerobic bacteria that is widely found in soil and water. Bacillus spp. can cause food poisoning and intestinal anthrax in humans.
To prevent bacterial contamination of mushrooms, cultivators can soak their grains for 12 to 24 hours at room temperature before sterilisation. This causes any bacterial endospores on the grains to awaken and germinate, and they are then killed during the sterilisation process. If bacterial contamination occurs, it can be treated with pasteurisation and sterilisation. Pasteurisation is an effective method for destroying most Gram-negative organisms, although some bacteria may survive and grow slowly after pasteurisation.
To prevent contamination, it is important to maintain a sterile environment when cultivating mushrooms. This includes sterilising the substrate and using air filtration to prevent impurities in the air, soil, or water from contaminating the mushrooms. Overall, it is important to identify and treat bacterial contamination in mushrooms promptly to prevent it from spreading and affecting neighbouring dishes, jars, bags, and tubs.
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Fungi, including Trichoderma, can be identified by discolouration and treated with salt
Mushrooms are vulnerable to various types of contamination, including bacteria, mould (fungi), and pests. Fungi, including Trichoderma, can be identified by discolouration and treated with salt.
Bacterial contamination, such as Bacillus spp., often manifests as slimy, wet patches on the mushroom's mycelium, earning it the nickname "wet spot". This type of contamination can be prevented by soaking the grains in water for 12 to 24 hours before sterilisation, causing any heat-resistant bacterial endospores to germinate and subsequently die during the sterilisation process.
Discolouration is a telltale sign of fungal contamination. While healthy mushroom mycelium should appear completely white, the presence of other colours, excluding blue (indicative of bruising) and yellow (a sign of ageing), suggests fungal growth. Trichoderma harzianum, in particular, produces an aggressive white mycelium that causes soft decay in mushrooms before it sporulates into a vibrant green, making it challenging to contain.
To address fungal contamination, salt can be effective. In mushroom production, spreading salt on affected areas is a strategy employed to combat Trichoderma. Additionally, salt has been found to inhibit the growth and infective capacity of certain fungi, such as the amphibian chytrid fungus, increasing host survival rates.
While salt can be used to combat fungal growth, it is important to note that simply cutting away and isolating the contaminated areas may be a more effective approach to prevent the spread of contamination to neighbouring dishes, jars, or bags. Sanitising the affected area and equipment is crucial to prevent future contamination.
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Pests, tiny insects that transmit disease, can be controlled with strict sanitation and an airtight grow room
Mushroom contamination can occur due to bacteria, mould, and pests. Pests, in particular, can be tiny insects that transmit diseases and devour the mushroom culture.
To control pests, strict sanitation and an airtight grow room are necessary. Here are some measures to achieve this:
Regular Monitoring and Inspection
Regularly monitor and inspect your mushroom farm for early signs of pests or diseases. Look for visible insects, eggs, larvae, or any abnormalities. Check for physical damage to the mushrooms, such as holes or bite marks, which can indicate pest presence.
Sterilization and Pasteurization
Ensure a clean growing environment by using sterile growth mediums and pasteurizing the soil. Substrate sterilization and air filtration can help create a sterile environment. However, it's important to remember that these methods are not foolproof, and the duration of sterility is limited.
Natural Pest Control
Introduce natural enemies of pests, such as predators, parasites, and pathogens. For example, beneficial insects like ladybugs, lacewings, or parasitic wasps can be released to control pests like aphids, mites, and fungus gnats. Beneficial nematodes can also be used to control fungus gnats.
Companion Plants
Companion plants like basil, marigolds, or chives can be strategically planted around the mushroom cultivation area to repel certain insect pests. These companion plants mask the scent of the mushrooms, confusing and deterring pests.
Integrated Pest Management (IPM)
Implement an Integrated Pest Management (IPM) strategy, which combines multiple pest control methods for a more effective solution. This includes regular monitoring, prevention, and control methods, aiming to minimize the use of chemical pesticides.
By following these measures, you can effectively control pests and create a thriving, pest-free mushroom crop.
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Human contamination, the greatest source of contamination, can be mitigated with hand washing, sanitiser, clean clothes, face masks, and gloves
Mushrooms are highly susceptible to contamination, which can be caused by anything from bacteria and moulds to pests. The human body is the greatest source of contamination in mushroom cultivation. Human hands and clothes carry a lot of bacteria and spores that can ruin a crop. However, there are several ways to mitigate human contamination.
Firstly, personal hygiene is crucial. Before handling mushrooms, it is important to maintain good hand hygiene by washing hands thoroughly with soap and water or using an alcohol-based hand sanitiser. This helps eliminate any bacteria or spores that may be present on the hands. Additionally, taking a shower before working with mushrooms can further reduce the risk of contamination.
Secondly, wearing clean clothes is essential. It is recommended to change into fresh clothing, such as a lab coat or scrubs, before entering the mushroom cultivation area. This ensures that any bacteria or spores on everyday clothing are not transferred to the mushrooms.
Another measure to prevent human contamination is the use of personal protective equipment (PPE). Wearing a face mask or a mouth covering helps prevent the spread of respiratory droplets that may contain bacteria or spores. Additionally, laboratory gloves can be worn to create a barrier between the hands and the mushrooms, reducing the risk of transferring contaminants.
By following these measures, such as hand washing, using sanitisers, wearing clean clothes, and donning face masks and gloves, the risk of human contamination in mushroom cultivation can be significantly reduced. These practices help maintain a sterile environment, which is crucial for the healthy growth of mushrooms and preventing crop loss due to contamination.
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Airborne contamination, including mould spores, can be reduced with a laminar flow hood or still air box
Contamination is a common issue in mushroom cultivation. It can be caused by bacteria, mould, or pests, and can quickly spread and ruin crops. The human body is the greatest source of contamination, as human hands and clothes can carry bacteria and spores. Airborne contamination, including mould spores, is also a significant concern.
To reduce the risk of airborne contamination, mushroom growers can use a laminar flow hood. This is a workbench with a built-in filter pad, fan, and High Efficiency Particulates Air (HEPA) filter. The laminar flow hood provides a clean stream of air for mycological work and can effectively eliminate airborne contaminants. However, it is quite costly and usually only used by dedicated mycologists and commercial growers.
If a laminar flow hood is not feasible due to budget constraints, a still air box (SAB) is a good alternative. A still air box is a large clear tote with arm holes cut into the sides. The inside of the tote can be cleaned with alcohol, and the user can slide their hands through the holes to perform the work. This option is more affordable and is popular for small-scale projects and home cultivators.
While these tools can help reduce airborne contamination, it is important to note that achieving perfect sterility in mushroom cultivation is nearly impossible. Other sources of contamination include cultivation tools, breath, and even the mushroom cultures or substrates themselves. Therefore, it is crucial to take multiple precautions and maintain a clean and sterile environment to minimize the risk of contamination.
Additionally, it is important to be able to identify the early signs of contamination. Discolouration is one of the most obvious signs, as contaminants often display distinct and bright colours. Slimy patches on grains or mycelium can indicate excess moisture and possible bacterial contamination. Unusual odours can also be a sign of contamination, such as mouldy or musty smells.
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Frequently asked questions
Mushroom contamination occurs when there is an unwanted presence in the mushroom substrate or mycelium that negatively impacts its growth. Contaminants can include bacteria, mould, and animal pests.
Discolouration is one of the most obvious signs of contamination. Yellow or brown spots or streaks on the surface of the mushrooms or growing medium, for example, may indicate contamination. However, blue stains may be natural bruising, and yellow may indicate the mycelium is getting old. Other signs include a strange odour, such as a mouldy or musty smell, and slime, which may indicate bacterial contamination.
Trichoderma harzianum is the most common fungus contaminating mushrooms. It produces an aggressive white mycelium that causes a soft decay in mushrooms before it sporulates into a vibrant green. The most common type of mould is Penicillium, which often appears on wood. The most common type of bacterial contamination is Bacillus spp., also known as "wet spot" or "sour rot".

























