
Growing mushroom trays requires careful consideration of the environment to ensure optimal conditions for mycelium development and fruiting. The ideal location should offer a controlled climate with stable temperatures between 60-75°F (15-24°C), high humidity levels around 80-90%, and proper ventilation to prevent mold and stagnant air. Dark or dimly lit spaces are preferable, as mushrooms thrive in low-light conditions. Common locations include basements, closets, or dedicated grow rooms equipped with humidity control systems and proper airflow. Additionally, the area should be clean and free from contaminants to avoid competing organisms. Whether using a home setup or a commercial facility, maintaining these conditions is crucial for successful mushroom cultivation.
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What You'll Learn
- Optimal Indoor Locations: Choose cool, dark spaces like basements, closets, or sheds with controlled humidity and airflow
- Outdoor Growing Spots: Use shaded areas under trees, in gardens, or on covered patios with indirect sunlight
- Temperature Requirements: Maintain 55–75°F (13–24°C) for most mushroom species to ensure healthy growth
- Humidity Control Tips: Place trays near humidifiers or in sealed containers to retain moisture levels
- Substrate Preparation: Use pasteurized straw, sawdust, or compost as a nutrient-rich base for mushroom trays

Optimal Indoor Locations: Choose cool, dark spaces like basements, closets, or sheds with controlled humidity and airflow
When selecting optimal indoor locations for growing mushroom trays, the key factors to consider are temperature, light, humidity, and airflow. Cool, dark spaces such as basements, closets, or sheds are ideal because mushrooms thrive in environments that mimic their natural habitat—shady, damp forest floors. Basements are particularly advantageous due to their naturally cooler temperatures, which typically range between 55°F and 65°F (13°C to 18°C), a range most mushroom species prefer. Ensure the chosen basement area is free from drafts and extreme temperature fluctuations, as stability is crucial for successful growth.
Closets can also serve as excellent mushroom-growing spaces, especially if they are spacious and can be modified to control humidity and airflow. Install a small fan to maintain gentle air circulation, which prevents the buildup of carbon dioxide around the mushrooms and reduces the risk of mold. Use a hygrometer to monitor humidity levels, aiming for 70-90%, and consider adding a humidifier or tray of water if the air is too dry. Keep the closet door slightly ajar or use a vent to allow for minimal light exposure while maintaining darkness, as excessive light can hinder mushroom development.
Sheds are another viable option, particularly for those with limited indoor space, but they require more attention to environmental control. Insulate the shed to maintain cooler temperatures and use a space heater or air conditioner as needed to keep the environment within the optimal range. Install a dehumidifier or humidifier to regulate moisture levels, and ensure the shed has proper ventilation to prevent stagnant air. Cover windows with blackout curtains or opaque materials to create the dark conditions mushrooms need to flourish.
Regardless of the location—basement, closet, or shed—consistency is key. Fluctuations in temperature, humidity, or airflow can stress the mushrooms and reduce yields. Regularly monitor these conditions and adjust as necessary. Additionally, keep the growing area clean and free from contaminants by sanitizing equipment and surfaces. By choosing and maintaining a cool, dark space with controlled humidity and airflow, you create an ideal environment for mushroom trays to thrive, ensuring healthy and abundant harvests.
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Outdoor Growing Spots: Use shaded areas under trees, in gardens, or on covered patios with indirect sunlight
When considering where to grow mushroom trays outdoors, shaded areas under trees, in gardens, or on covered patios with indirect sunlight are ideal. Mushrooms thrive in environments that mimic their natural habitats, which are often cool, humid, and protected from direct sunlight. Shaded areas under trees provide a natural canopy that filters sunlight, creating the perfect balance of light and darkness. Ensure the spot is free from strong winds, as mushrooms require stable conditions to grow. The soil under trees is often rich in organic matter, which can benefit mushroom growth, but avoid areas where the soil is overly compacted or waterlogged.
Gardens are another excellent outdoor spot for growing mushroom trays, especially if you have a dedicated section with partial shade. Choose a spot where plants or structures, like fences or shrubs, block direct sunlight during peak hours. Gardens often have good air circulation, which helps prevent mold and mildew on your mushroom trays. Incorporate organic mulch or compost into the soil to enhance moisture retention and provide nutrients. Regularly monitor the area to ensure it remains shaded and humid, as mushrooms are sensitive to drying out.
Covered patios or decks with indirect sunlight are also great options for outdoor mushroom cultivation. These areas offer protection from rain and excessive sunlight while still allowing for adequate air exchange. Place your mushroom trays on tables or stands to keep them off the ground, reducing the risk of pests or water damage. Use shade cloth or mesh to further diffuse sunlight if the patio receives too much light. Maintain humidity by misting the area or placing a tray of water nearby, as patios can sometimes be drier than other outdoor spots.
When selecting any of these outdoor growing spots, consider the microclimate of the area. Mushrooms prefer temperatures between 55°F and 70°F (13°C and 21°C), so avoid locations that get too hot or cold. Monitor the humidity levels, aiming for 60-80%, and adjust as needed. For shaded areas under trees or in gardens, ensure the trays are easily accessible for watering and harvesting. On covered patios, ensure proper drainage to prevent water from pooling around the trays. With the right spot and care, these outdoor areas can become productive environments for growing mushrooms.
Lastly, prepare the growing area by ensuring it is clean and free from contaminants. Sterilize your mushroom trays and tools before placing them outdoors to minimize the risk of infection. If using a garden or under-tree spot, consider laying down a ground cover like straw or landscape fabric to keep the trays clean. For patios, elevate the trays to allow air circulation underneath. Regularly inspect the area for pests or diseases and address any issues promptly. By carefully selecting and preparing these shaded outdoor spots, you can create an optimal environment for your mushroom trays to flourish.
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Temperature Requirements: Maintain 55–75°F (13–24°C) for most mushroom species to ensure healthy growth
Temperature control is a critical factor when considering where to grow mushroom trays, as it directly impacts the success and yield of your mushroom cultivation. Most mushroom species thrive within a specific temperature range, typically between 55–75°F (13–24°C). This range ensures that the mycelium, the vegetative part of the fungus, grows optimally and that fruiting bodies (mushrooms) develop healthily. Maintaining this temperature range is essential because deviations can lead to slow growth, contamination, or even the failure of the crop. Therefore, selecting a location that allows for consistent temperature regulation is paramount.
When choosing a space to grow mushroom trays, consider areas that naturally stay within the 55–75°F (13–24°C) range, such as basements, cellars, or climate-controlled rooms. Basements, for instance, often provide a stable environment due to their insulation from external temperature fluctuations. If natural conditions are not sufficient, investing in heating or cooling equipment, such as space heaters, fans, or air conditioners, can help maintain the desired temperature. Additionally, using thermostats or temperature monitors ensures you can track and adjust conditions as needed, providing a stable environment for mushroom growth.
For those growing mushrooms in warmer climates or during hotter seasons, it’s crucial to implement cooling strategies. Placing mushroom trays in shaded areas or using evaporative coolers can help lower temperatures to the optimal range. Conversely, in colder environments or seasons, insulation and supplemental heating are necessary. Insulated grow rooms or the use of heat mats can provide the warmth required to keep temperatures within the 55–75°F (13–24°C) range. Always avoid placing trays near drafts, windows, or doors that may expose them to extreme temperature changes.
Consistency is key when it comes to temperature requirements for mushroom cultivation. Fluctuations outside the 55–75°F (13–24°C) range, even for short periods, can stress the mycelium and hinder growth. For example, temperatures above 75°F (24°C) can cause the mycelium to become dormant or attract contaminants, while temperatures below 55°F (13°C) can slow growth significantly. Therefore, the chosen location should provide a stable environment, free from sudden temperature shifts. Regular monitoring and adjustments will ensure that your mushroom trays remain within the ideal range for healthy development.
Lastly, consider the scalability of your growing space. If you plan to expand your mushroom cultivation, ensure the area can accommodate additional trays while still maintaining the 55–75°F (13–24°C) temperature range. Larger spaces may require more robust heating or cooling systems to regulate temperature effectively. By carefully selecting and preparing your growing location, you can create an environment that supports the temperature requirements of most mushroom species, leading to successful and bountiful harvests.
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Humidity Control Tips: Place trays near humidifiers or in sealed containers to retain moisture levels
Maintaining optimal humidity is crucial for successfully growing mushrooms in trays. One effective strategy is to place trays near humidifiers. Humidifiers release moisture into the air, creating a humid environment that mushrooms thrive in. Position your trays within a few feet of the humidifier to ensure they benefit from the increased moisture levels. This method is particularly useful in dry climates or during seasons when indoor air tends to be drier. However, monitor the humidity closely to avoid over-saturation, which can lead to mold or other issues.
Another practical approach is to place trays inside sealed containers. This method traps moisture around the mushroom trays, creating a microclimate with consistent humidity. Use clear plastic containers with lids or DIY setups like large storage bins. Ensure the container is large enough to accommodate the trays with some airspace but not so large that humidity control becomes difficult. Periodically check the trays to ensure proper air exchange while maintaining high humidity. This technique is especially effective for small-scale growers or those with limited space.
Combining both methods can yield even better results. Pairing humidifiers with sealed containers maximizes humidity control, providing a stable environment for mushroom growth. Place a humidifier near the sealed container to continuously replenish moisture lost through minimal air exchange. This setup is ideal for growers aiming for precision and consistency, particularly when cultivating sensitive mushroom varieties that require strict humidity levels.
When using these techniques, regularly monitor humidity levels with a hygrometer to ensure they remain within the optimal range for your mushroom species (typically 85-95% relative humidity). Adjust the humidifier settings or container ventilation as needed. Additionally, avoid placing trays in drafty areas or near heat sources, as these can disrupt humidity levels. Proper placement and monitoring will significantly enhance the success of your mushroom cultivation efforts.
Finally, consider the material of your trays and containers when implementing these tips. Non-porous materials like plastic or glass are best for retaining moisture, while porous materials may absorb water, affecting humidity levels. Clean and sanitize all equipment regularly to prevent contamination. By strategically placing trays near humidifiers or in sealed containers, you can create an ideal environment for healthy mushroom growth while minimizing the risk of common humidity-related issues.
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Substrate Preparation: Use pasteurized straw, sawdust, or compost as a nutrient-rich base for mushroom trays
When preparing the substrate for mushroom trays, selecting the right base material is crucial for successful growth. Pasteurized straw is one of the most commonly used substrates, especially for oyster mushrooms. To prepare straw, soak it in water for a few hours to rehydrate it, then pasteurize it by soaking in hot water (around 160-180°F or 71-82°C) for about an hour. This process eliminates competing microorganisms while retaining enough nutrients for mushroom mycelium. After pasteurization, drain the straw thoroughly and allow it to cool before using it as a base in your trays. Proper moisture content is key—the straw should feel damp but not waterlogged.
Sawdust is another excellent substrate, particularly for shiitake and other wood-loving mushroom species. To prepare sawdust, mix it with a nutrient supplement like wheat bran or soy hulls to provide additional nitrogen. Pasteurization is also necessary for sawdust; this can be done by steaming it for 2-3 hours or using a hot water bath similar to straw. Once pasteurized, let the sawdust cool and ensure it is evenly moist before filling your trays. Sawdust substrates often require more attention to detail, as they can compact easily, restricting air flow, so consider adding a small amount of gypsum to improve structure.
Compost serves as a nutrient-dense substrate, ideal for button mushrooms and other agaricus species. Preparing compost involves creating a mixture of straw, hay, and horse or poultry manure, which is then allowed to decompose and pasteurize through a process called Phase II composting. This step raises the temperature of the compost to kill pathogens and competing fungi. After pasteurization, the compost should be cooled and adjusted to the correct pH (around 6.8-7.2) before being used in trays. Compost substrates are rich in organic matter, providing ample nutrients for robust mushroom growth.
Regardless of the substrate chosen, uniformity and cleanliness are essential. Ensure all materials are free from pesticides or contaminants that could harm mycelium. After pasteurization, allow the substrate to cool to room temperature before inoculating with spawn, as high temperatures can kill the mushroom culture. Properly prepared substrates should be evenly moist, with a moisture content of 60-70%, and well-aerated to support healthy mycelium colonization. Following these steps will create an optimal environment for mushroom growth in your trays.
Finally, consider the specific needs of the mushroom species you are cultivating, as some may require additional amendments or specific substrate conditions. For example, some growers add lime to compost for button mushrooms to stabilize pH. Always sterilize or pasteurize your tools and trays before use to maintain a sterile environment. With careful substrate preparation, you’ll provide a strong foundation for your mushroom trays, setting the stage for a bountiful harvest.
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Frequently asked questions
Most mushrooms thrive in temperatures between 60°F and 75°F (15°C and 24°C). Ensure consistent temperature to promote healthy growth.
Choose a cool, dark, and humid area like a basement, closet, or under a sink. Avoid direct sunlight and drafts to maintain optimal conditions.
Basic equipment includes trays, a growing medium (like straw or sawdust), mushroom spawn, and a spray bottle for misting. A humidifier or DIY humidity tent can also help.
Mist the trays lightly 1-2 times daily to maintain humidity. Avoid overwatering, as excess moisture can lead to mold or rot.

























