
Mushrooms are known to be nature's healers, and they can help decompose toxic substances, including diesel. However, diesel contamination is a common issue for diesel fuel users, and it can lead to serious engine problems. This contamination is caused by microbes such as bacteria and fungi, which thrive in the presence of water and certain types of fuel. While some products claim to kill these microbes, it is unclear how effective they are. To prevent contamination, it is recommended to keep fuel tanks full and use the vehicle regularly. In addition, mycoremediation, which uses mushrooms to decompose toxic substances, has been explored as a potential solution to diesel contamination.
| Characteristics | Values |
|---|---|
| Diesel contamination by microbes | Bacteria, fungi, algae |
| Contaminants enter diesel via | Condensation, rainwater penetration, adsorption from the air |
| Contaminants thrive in | Above-ground plastic tanks, fuel tanks exposed to a wide range of temperatures |
| Contaminants are prevented by | Sulphur in diesel, Biobar, Bio Kleen Diesel Fuel Biocide, Clear-Diesel, Biobor, Ameristats, electro magnets, UV LEDs, anti-bacterial diesel additive, shock dosing |
| Contaminants cause | Malfunctioning, bad smell, clogged filters, fuel injectors, engine shut-down, engine wear, fuel degradation, reduced combustion efficiency, poor detergency, elevated cloud and pour point |
| Mushrooms help with | Mycoremediation, decomposing toxic substances, habitat recovery, filtration systems, breaking down hydrocarbon-based toxic substances |
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What You'll Learn

Diesel fuel contamination
One of the main contributors to diesel fuel contamination is water. Water can enter the fuel through condensation, temperature changes, or direct exposure to rain or snow. Even a small amount of water can enable microbiological contamination as microbes can develop and thrive in the presence of water and the hydrocarbons in diesel fuel. This microbial growth, also known as diesel bug or diesel fungus, can cause serious damage to engines and fuel systems, leading to corrosion, blockages, and even engine failure.
To prevent and address diesel fuel contamination, several methods can be employed:
- Fuel Additives: Using fuel additives or biocides, such as Killem or Sta-Bil, can help kill microbial growth and prevent further contamination. However, it is important to carefully select and use these products as some may have adverse effects on certain engine components.
- Filtration and Polishing: Circulating the diesel fuel through external filters or a fuel polishing service can help remove water, dead bacteria, and debris from the fuel and fuel tanks.
- UV Treatment: Implementing UV LEDs or treatment units inside diesel tanks can help prevent biological contamination by inhibiting the growth of microorganisms.
- Proactive Fuel Management: Regularly using and maintaining diesel engines, as well as storing fuel under optimal conditions, can reduce the risk of contamination. Additionally, paying attention to fuel management issues and staying informed about recent changes in diesel fuel compositions can help users make informed decisions to mitigate contamination risks.
- Cleaning and Maintenance: In some cases, it may be necessary to drain and clean the fuel tanks, removing accumulated sediment and contaminants. Services specializing in tank cleaning and fuel restoration can be engaged to perform these tasks effectively.
It is important to note that while these methods can help prevent and address diesel fuel contamination, early detection is crucial to minimize potential damage and costs associated with engine repairs and downtime. Regular monitoring of fuel quality and engine performance can help identify contamination issues before they become critical.
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Anti-bacterial additives
Diesel fuel is susceptible to bacterial and fungal contamination, which can cause serious damage to engines and systems. This contamination often occurs due to the presence of water in fuel tanks, which can result from condensation, water penetration, or absorption from the air. Biodiesel, a common component in modern diesel fuels, is hygroscopic, meaning it attracts water molecules from the atmosphere, further increasing the risk of contamination.
To combat this issue, anti-bacterial diesel additives are available as a preventive measure. These additives are designed to inhibit the growth of bacteria, yeast, and mould, and are especially recommended for diesel-powered vehicles that are decommissioned for extended periods or are infrequently used. They can also be used to disinfect infested tank systems. When used proactively, these additives serve as a cost-effective solution, preventing the need for complex repairs or engine cleaning.
One such additive is LIQUI MOLY's Anti-bacterial Diesel Additive, which is compatible with all conventional diesel and biodiesel fuels. It is a highly effective biocide with a broad spectrum of activity against bacteria, yeast, and mould. For preventive use, 25 ml of the additive is sufficient for 25 litres of diesel fuel. However, in cases of severe contamination, a booster dose of 1:200 is recommended.
Another popular option is Bio Kleen Diesel Fuel Biocide Treatment, which is widely used in North America across various industries, including construction and mining. This biocide is effective in both diesel fuel and water, targeting and killing microbes in fuel, including bacteria and fungus. By maintaining fuel tank hygiene, this additive helps improve diesel engine performance and ensures trouble-free operability.
While these anti-bacterial additives offer a solution to microbial contamination in diesel fuel, it is important to exercise caution when using biocides. Users should always read the label and product information before use and follow the recommended dosage instructions. Additionally, some individuals have suggested alternative methods, such as using UV LEDs or electro magnets to prevent and treat contamination.
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Sulphur and biodiesel
Biodiesel is derived from various feedstocks such as peanut oil, soybean oil, corn oil, canola oil, etc. However, such feedstocks require large areas of fertile land, fresh water, and the biodiesel production based on them competes with human food. Sulphur analysis is an important step in the production of biodiesel. Sulphur in diesel helps prevent bacteria. Low sulphur diesel is more susceptible to contamination than high sulphur diesel as the sulphur kills the bacteria.
The biodiesel industry has successfully transitioned to using yellow grease, a waste material from frying food. Making biodiesel from lower-grade waste streams such as FOG (fat, oil, and grease) is an attractive pathway that reduces feedstock costs and solves a difficult waste management problem. FOG is created from cooking byproducts mixed with water. Brown grease is derived from FOG by removing most of the water, leaving fats and oils, some water, suspended solids, and other contaminants.
The β-CD system is a new desulfurization method applied to biodiesel that uses β-cyclodextrin as the adsorbent. It operates at ambient temperature and pressure, whereas the vacuum distillation system operates at high temperatures and low pressure. The initial cost of the β-CD system is expected to be less than half the cost of the distillation system, but the operating costs may be similar. The β-CD system does not require the addition of hydrogen as in hydrodesulfurization but does require regeneration and periodic replacement of β-CD.
Biodiesel gained widespread attention starting in the 1980s as a replacement for petroleum diesel amid rising oil prices. As a diesel-equivalent, processed fuel derived from biological sources has been used as a blending component or a direct replacement for diesel fuel in vehicle engines.
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Fuel storage
Diesel fuel should be able to sit for at least two years without degrading. However, if your fuel is subjected to variations in weather and temperature, or has been stored for several months, it may become contaminated. Water is the most common contaminant, usually building up from condensation. Biodiesel is hygroscopic, meaning it attracts water molecules from atmospheric moisture. Sulphur in diesel helps to prevent bacteria, but low-sulphur diesel is more susceptible to contamination as it lacks this natural preventor.
To avoid contamination, it is recommended to buy fuel from a name-brand station that turns over fuel, preferably from newer tanks, and to keep your tank as full as possible. This will reduce the amount of space in the tank in which moisture can accumulate.
If your fuel does become contaminated, there are several ways to address the issue:
- Pay someone to recirculate the diesel through external filters and filter it.
- Use a product like Biobar or Biobor to shock the fuel and kill the bacteria, then filter out the dead bacteria and debris.
- Use a biocide like Killem from FPPF to dose your fuel tanks.
- Use electro magnets around the fuel intake lines to break up waxy, non-fungal solid clusters that form in stored diesel.
- Have your tanks professionally cleaned, which will typically cost a few hundred dollars.
- Drain the tank, steam clean it, and refill with fresh fuel.
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Mycoremediation
Fungi have been used to remediate tracts of land contaminated by mining or other human activities. For example, in one experiment, a field that was inoculated with oyster mushroom spores biodegraded the most toxic oil and replaced what was once a contaminated wasteland with a thriving ecosystem. This experiment took the soil’s PAH count (the concentration of this often carcinogenic aromatic hydrocarbon) from 10,000 parts per million (ppm) to less than 200 ppm in eight weeks.
Fungi such as Pleurotusdryinus, Trameteshirsuta MK640786, and Aspergillusniger have shown degradation of pollutants ranging from 91% to 98.4%. They can also remove heavy metals and emerging pollutants from water and soils, such as pesticides, pharmaceuticals, polycyclic aromatic hydrocarbons (PAHs), and weedicides.
In addition to the benefits of mycoremediation, it is important to consider the potential drawbacks and limitations. For instance, the role of different fungal species in the degradation process is still not fully understood and requires further research. Additionally, the effectiveness of mycoremediation may depend on the type of hydrocarbon fuel being remediated, with some fuels being more resistant to fungal degradation than others.
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Frequently asked questions
Diesel mushrooms are a result of diesel bug, which is contamination of diesel fuel by microbes such as bacteria and fungi.
Water can enter diesel fuel through condensation, rainwater penetration, or adsorption from the air. This water can start the growth of bacteria, which breeds and multiplies quickly, eventually plugging and blocking a fuel system.
The bacteria and fungi reproduce and form biomass, which accumulates at the fuel-to-water interface, on tank surfaces, and filters. As the biomass breaks down, sludge forms at the bottom of the tank. This sludge can clog fuel injectors and filters, leading to engine shutdown due to fuel starvation.
To prevent diesel mushroom formation, you can use anti-bacterial diesel additives, biocides, or UV LEDs in the diesel tank. Keeping your fuel tanks full and regularly using your vehicle can also help prevent water accumulation and microbial growth.
Oyster mushrooms (Pleurotus ostreatus) have been found to decompose diesel and other toxic substances in soil through a process called mycoremediation.

























