
Mushroom cultivation is a complex process that requires the right tools and techniques to ensure success. One of the critical decisions growers face is choosing the appropriate type of bag for their mushrooms. While various options are available, such as polypropylene grain bags and traditional methods, the use of filter bags has gained popularity due to their ability to provide optimal conditions for mushroom growth. These filter bags come in different varieties, such as micron filter patch bags, zipper filter bags, and unicorn bags, each offering unique advantages for cultivating healthy mushrooms. The choice of bag and filter type depends on several factors, including the species of mushroom, the substrate used, and the desired level of airflow and gas exchange during the cultivation process.
| Characteristics | Values |
|---|---|
| Purpose of filter | Allow mycelium to breathe while keeping moisture and contaminants out |
| Filter options | Micron filter patches, Uline gusseted bags, filter strips, collar and cotton plug |
| Filter pore size | 0.2, 0.5, 2-5 microns |
| Filter patch placement | Top 20% of the bag, 2x3-4 inches |
| Filter patch material | Polypropylene |
| Bag material | Polypropylene, Polythene |
| Bag type | Unicorn bags, zipper filter bags, lay-flat tubing, polypropylene grain bags, co-bags |
| Autoclave temperature | 250 °F |
| Autoclave pressure | 15 PSI |
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What You'll Learn

The role of filters in mushroom bags
Filters play a crucial role in mushroom cultivation, ensuring optimal conditions for mushroom growth. The type of bag used for cultivation is important, and mushroom growers can choose from various options, including traditional methods using polypropylene grain bags or more modern solutions like mushroom co-bags.
The primary function of filters in mushroom bags is to facilitate gas exchange while preventing contaminants from entering. Micron filter patches, for instance, allow for gas exchange and help maintain the right temperature and humidity levels necessary for mushroom growth. These filters have varying pore sizes, with smaller pores offering tighter protection against bacteria and spores but also restricting airflow more. For instance, a 0.2-micron filter allows only the smallest molecules to pass through, including air, but at a slower rate. In contrast, larger pore sizes provide increased airflow but carry a higher risk of competitor organisms infiltrating the bag.
The choice between filter types depends on the specific needs of the grower and the species being cultivated. For instance, the Unicorn Type T Filter, with its 0.2-micron pores, is ideal for spawn production, while a larger pore size may be preferable for faster-growing mushroom species.
Additionally, filter placement and shape can vary in mushroom bags. The average bag features a filter patch placed towards the top 20% of the bag, measuring roughly 2 by 3 to 4 inches. This design keeps the filter away from the bag's contents and allows sufficient airflow. Horizontal filters, spanning a larger width, are gaining popularity as they facilitate better fruiting conditions by allowing more air into the bag.
Overall, filters in mushroom bags are essential for successful cultivation, providing the necessary gas exchange while maintaining a sterile environment for mushrooms to thrive.
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Types of filters
The type of bag used for mushroom cultivation plays a crucial role in the growth process. The market offers a variety of mushroom growing bags, and choosing the right one can be confusing. Here is a guide to help you select the most suitable filter for your mushroom bag:
Filter Patch Bags
Micron filter patch mushroom grow bags are an excellent choice for mushroom cultivation as they allow gas exchange while preventing contaminants from entering the bag. These bags have a filter patch that enables the mushroom mycelium to breathe while growing. They come in various shapes and sizes, with different pore sizes to suit all mushroom-growing techniques. The pore size of the filter patch plays a vital role in maintaining optimal conditions for mushroom growth. For instance, a pore size of 5 microns allows you to seal the bags before autoclaving, preventing competing fungi from entering.
Unicorn Bags
Unicorn Bags is a popular supplier of high-quality filter patch bags. Their Unicorn Type T Filter with 0.2-micron pores is specifically designed for spawn production, providing excellent filtration performance with 90% efficiency. For shiitake cultivation, they offer the Type A filter bag with a nominal pore size of 0.5 microns, creating an environment conducive to shiitake mycelium growth in higher CO2 levels.
Zipper Filter Bags
Zipper filter bags, produced by SacO2, are autoclavable and have filters to allow the mycelium to breathe. Instead of a patch, they feature filter strips covering the entire length of the bag, optimising fresh air exchange. SacO2 offers different filters for spawn and substrate, and you can choose from various bag and filter sizes.
Collar and Cotton Plug
Some growers use a collar and cotton plug for air exchange instead of a filter. This method involves filling 3/4 of the bag with the substrate, folding the top over, and sterilising it. After sterilisation, the top of the bag is opened in a glove box, and the spawn or culture is inoculated, along with the collar and cotton plug.
DIY Filters
For beginners, an easy way to try mushroom cultivation without purchasing specialised bags is to use large ziplock freezer bags. You can make a breathable filter in these bags by cutting a 0.8-inch (2 cm) hole and filling it with Polyfill.
It is important to note that the ideal filter size depends on the mushroom species being grown and whether you plan to use the bag for spawn or substrate. Larger pore sizes provide increased air exchange but carry a higher risk of competitor organisms entering. Therefore, larger filters are generally recommended for faster-growing species to reduce the chances of contamination associated with larger filter sizes.
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Factors to consider when choosing a filter bag
When choosing a filter bag for mushroom cultivation, there are several factors to consider. The type of bag used for mushroom cultivation plays an important role in the success of the process. Here are some key factors to consider when selecting a filter bag:
Type of Mushroom and Growth Stage
The type of mushroom and the stage of growth are important factors when choosing a filter bag. Different mushroom species have specific requirements for optimal growth. Additionally, the filter bag should be suitable for the stage of mushroom cultivation, such as grain colonization, substrate colonization, or fruiting. Some filter bags, like the Unicorn Type T Filter, are specifically designed for spawn production and offer excellent filtration performance.
Filter Efficiency and Pore Size
Filter efficiency and pore size are crucial considerations. Filters with smaller pore sizes, such as 0.2-micron filters, offer tighter filtration, allowing only the smallest molecules like air to pass through while blocking bacteria and spores. These filters are ideal for maintaining a sterile environment and can be used in various growth stages. On the other hand, larger pore sizes, such as 2-5 micron filters, provide increased air exchange but carry a higher risk of contaminants entering the bag.
Bag Size and Thickness
The size and thickness of the filter bag are important factors. The bag size should be chosen based on the available space and the desired quantity of mushrooms to be grown. Thicker bags, like those with a thickness of 4 mil (100 microns), are less likely to tear but tend to be more expensive. For beginners, it is recommended to start with thinner and less expensive bags, such as 2.2 mil (56 microns) bags, and handle them with care.
Autoclavability and Sterilization
If sterilization is required, choose autoclavable filter bags that can withstand high temperatures. Polypropylene bags are a great choice for this purpose as they can withstand temperatures up to 270°F (132°C) without melting. Autoclavable bags are necessary if you plan to sterilize your substrate. However, if you're using the pasteurization method, you can opt for regular plastic bags with holes poked in them for air exchange.
Bag Design and Features
Consider the design and additional features of the filter bag. Gusseted bags, for example, have a pleated design that expands to accommodate growing mycelium. Some bags offer injection ports for injectable mushroom genetic material, which can be useful depending on your cultivation method. Additionally, look for bags with proper insulation and ventilation to maintain optimal temperature and humidity levels for mushroom growth.
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The importance of temperature and airflow
Temperature and airflow are critical factors in mushroom cultivation. Mushrooms require specific temperature ranges to grow, and airflow helps maintain these temperatures. Oyster mushrooms, for instance, require a temperature range of 65-75°F, while Shiitake mushrooms require a slightly cooler range of 65-75°F. In addition, airflow provides the necessary gas exchange for mushroom respiration and energy production.
Proper airflow facilitates the exchange of oxygen and carbon dioxide, which is essential for mushroom growth. It also helps regulate moisture levels, as it allows excess water to evaporate, maintaining optimal humidity levels. This is crucial as mushrooms require high humidity to initiate growth and develop into mature mushrooms.
The type and size of mushroom bags can impact airflow and, consequently, temperature and humidity levels. Some bags are designed with micro-perforations or breathable filter patches to enhance airflow, prevent moisture buildup, and provide gas exchange. These bags help maintain a consistent temperature and reduce the risk of contamination by allowing fresh air in while keeping contaminants like mould spores and bacteria out.
To further regulate temperature and airflow, cultivators can adjust the substrate moisture content. Reducing substrate moisture can decrease humidity levels, and this can be achieved by increasing airflow. Additionally, air conditioning systems can be used to decrease the temperature, but it is important to make gradual adjustments to avoid shocking the mushrooms and impacting their growth.
In summary, temperature and airflow are vital for successful mushroom cultivation. Airflow provides the necessary gas exchange and helps regulate temperature and humidity levels. The design of the mushroom bags and substrate moisture content also play a role in maintaining optimal conditions for mushroom growth.
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Alternative methods to using filter bags
Filter bags are commonly used in mushroom cultivation to maintain optimal conditions for mushroom growth. These bags are designed with filter patches that allow the mushroom mycelium to breathe while preventing contaminants like mould, spores, or bacteria from entering. While filter bags are widely used, there are alternative methods and solutions for growing mushrooms that do not rely on filter bags.
One alternative method is to use a collar and cotton plug for air exchange instead of a filter. In this approach, growers fill about three-quarters of the bag with the substrate, fold the top over, and sterilize the substrate. After sterilization, they open the bag in a controlled environment, inoculate with spawn or culture, and insert the collar and cotton plug into the top. This method eliminates the need for heat-resistant bags and is suitable for cultivating easy-to-grow mushrooms like oyster mushrooms.
Another option is to use large ziplock freezer bags, which can be purchased from a local grocery store. These bags are thicker and less likely to split, providing a simple solution for beginners who want to try mushroom cultivation without investing in specialised mushroom grow bags. To ensure breathability, small holes can be cut into the freezer bags, and materials like Polyfill can be used to cover the holes, acting as a makeshift filter.
For those cultivating shiitake mushrooms, cylindrical storage containers made from polypropylene offer a reusable and cost-effective alternative to filter patch bags. By drilling holes in the containers and using filter patches or Poly-Fil, growers can achieve adequate air exchange. These containers can withstand the temperature and pressure of a pressure cooker, making them a suitable option for mushroom cultivation.
Additionally, some growers have suggested using sterile medical tape to create custom filter patches on bags. This DIY approach involves cutting a hole in the bag and covering it with medical tape. To ensure a secure seal, a hot glue gun can be used to affix the tape to the bag. This method allows for customisation and can be adapted using materials like polyester or cotton discs.
Lastly, for those cultivating oyster mushrooms, a method called "lay flat tubing" can be employed. This involves using a long, continuous plastic tube made from Polythene or Polypropylene. Growers cut the tubing to the desired length, fill it with pasteurized straw substrate, tie the ends shut, and poke holes in the bag for the mycelium to breathe. This approach helps reduce plastic waste as it requires fewer bags compared to traditional methods.
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Frequently asked questions
Filters on mushroom bags allow the mycelium to breathe while keeping moisture inside and contaminants outside. They also allow for gas exchange while preventing competing fungi from entering the bag.
There are three common types of filters for mushroom bags: .2 micron filters, .5 micron filters, and 2-5 micron filters. The size of the filter depends on the species being grown and the purpose of the bag.
Yes, some growers use a collar and cotton plug for air exchange instead of a filter. Others use injection ports for the use of injectable mushroom genetic material.

























