
The world of mushroom supplements can be confusing for new consumers, with terms like dual extract and hot water extraction not shedding much light on the quality or benefits of the product. The extraction process is important because mushrooms have thick chitinous cell walls, which protect all their active compounds. The mushroom cell walls are made from an indigestible fiber called chitin, the same material found in crustacean and insect shells. Hot water extraction is used to remove beta-glucans from mushroom material and is the most common type of mushroom extraction.
| Characteristics | Values |
|---|---|
| Main use | To remove beta-glucans from mushroom material |
| Mushroom types | Cordyceps, Lion's Mane, Turkey Tail, shiitake, or maitake |
| Mushroom form | Raw mushroom material ground into a coarse powder |
| Extraction process | Add hot water to the powder, filter the liquid, concentrate the liquid into a powder using a vacuum, spray dry, sift the powder |
| Mushroom compounds | Water-soluble compounds (beta-glucans), alcohol-soluble compounds (triterpenoids, sterols, flavonoids) |
| Extraction temperature | 70-80°C |
| Dual extraction | Hot water extraction followed by alcohol extraction |
| Mushroom cell wall | Made of indigestible fiber called chitin |
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What You'll Learn
- Hot water extraction is a traditional method for preparing mushroom supplements
- It is used to remove beta-glucans from mushroom material
- Alcohol extraction is used to remove flavonoids, triterpenes, and other minor compounds
- Dual extraction uses both hot water and alcohol extraction methods
- Hot water extraction is the only clinically validated method for breaking polysaccharides out of cell walls

Hot water extraction is a traditional method for preparing mushroom supplements
Mushrooms have thick cell walls made of chitin, the same indigestible fibre found in crustacean and insect shells. This fibrous component acts as a barrier, trapping the therapeutic compounds within. Hot water extraction is the only clinically validated method to break down these polysaccharides and release the beneficial compounds, such as beta-glucans, from the cell walls. This process ensures that the final product contains a high concentration of the desired compounds.
The hot water extraction method is favoured by many herbalists and is commonly used for preparing mushroom supplements. Mushrooms such as Cordyceps, Lion's Mane, Turkey Tail, Shiitake, and Maitake are often extracted using hot water as their active ingredients, beta-glucans, are water-soluble. This method is simple and effective, making it a popular choice for supplement preparation.
However, it is important to note that not all mushrooms are suitable for hot water extraction alone. Some mushrooms, like Chaga and Reishi, have super-thick cell walls, requiring a dual extraction process with ethanol to fully unlock their active compounds. In these cases, hot water extraction is followed by an additional step with ethanol, ensuring a more comprehensive extraction.
While hot water extraction is a valuable technique, dual extraction methods that utilise both hot water and alcohol can provide a wider range of beneficial compounds. Alcohol helps break down fat-soluble compounds, such as triterpenoids and certain antioxidants, that hot water alone cannot extract. Therefore, the choice of extraction method depends on the specific mushroom and the desired compounds to be extracted.
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It is used to remove beta-glucans from mushroom material
Hot water extraction is used to remove beta-glucans from mushroom material. Beta-glucans are the main active ingredient in the majority of medicinal mushrooms, and they are water-soluble.
To extract beta-glucans from mushrooms, the mushroom material is first dried and ground into a coarse powder. Hot water is then added to the powder, and the liquid filtrate is filtered out from the solids. The liquid filtrate is then concentrated into a powder using a vacuum, and the final product is spray-dried. This process can be performed at varying temperatures, pressures, and extraction times, depending on the specific mushroom and desired yield. For example, a study on the extraction of beta-glucans from gray oyster mushrooms found that the optimal extraction conditions were at 140 °C, 0.92 MPa, and 40 minutes, with a corresponding extraction yield of 3.20 g/100 g.
Hot water extraction is a valuable technique for creating mushroom supplements with high beta-glucan content, which is beneficial for immune support. However, it is important to note that some mushrooms, such as Chaga and Reishi, have super-thick cell walls that require a dual extraction process with ethanol to fully unlock their active compounds.
Overall, hot water extraction plays a crucial role in the production of mushroom supplements by effectively removing beta-glucans from mushroom material, contributing to the creation of potent and beneficial medicinal products.
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Alcohol extraction is used to remove flavonoids, triterpenes, and other minor compounds
The process of extracting mushroom supplements involves exposing raw mushroom material to a solvent, which breaks down certain components and draws them out from the mushroom material. The solvent can then be removed, leaving only the desired components behind.
Hot water extraction is a traditional mushroom extraction process and is the most common. It is used to remove beta-glucans from mushroom material. Beta-glucans are water-soluble and are the main active ingredient in the majority of medicinal mushrooms.
Alcohol extraction, on the other hand, is used to remove flavonoids, triterpenes, and other minor compounds from mushroom material. These compounds are non-water-soluble and are typically isolated using alcohol as a solvent. During alcohol extraction, mushrooms are combined with alcohol and left to sit for several weeks to months. The alcohol breaks down the mushroom cell walls, which are made of chitin, and releases the non-water-soluble compounds.
Some mushrooms, like Reishi and Chaga, contain a high amount of alcohol-soluble compounds and benefit from dual extraction, which uses both hot water and alcohol. However, it is important to note that alcohol can harm beta-glucans, so the order of extraction is important. Herbalists typically recommend doing a hot water extraction first and then an alcohol extraction to avoid the solidification and filtration of beta-glucans.
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Dual extraction uses both hot water and alcohol extraction methods
Medicinal mushroom supplements are prepared using different extraction methods, including hot water extraction, alcohol extraction, and dual extraction. Dual extraction uses both hot water and alcohol extraction methods to create a potent mushroom product.
The process of extraction involves exposing raw mushrooms to a solvent, such as hot water or alcohol (ethanol), to release certain ingredients from the raw material. The vital compounds in medicinal mushrooms are mostly water-soluble or alcohol-soluble, making hot water and alcohol the most common solvents for mushroom extraction.
Hot water extraction involves heating coarse mushroom powder in hot water over several hours. This method is used to extract water-soluble compounds like beta-glucans and polysaccharides. Beta-glucans are the main active ingredient in many medicinal mushrooms, and they are released when heat is applied.
However, not all vital ingredients in mushrooms are water-soluble. Some compounds, like triterpenes, are not easily extracted with water alone. This is where alcohol extraction comes into play. Alcohol extraction is similar to water extraction but uses alcohol or ethanol as the solvent. This method can extract ingredients that are not water-soluble, such as triterpenoids, sterols, and flavonoids.
Dual extraction combines both hot water and alcohol extraction methods to create a more comprehensive product. In this process, the mushrooms are first extracted with hot water, and then the remaining plant material is extracted with alcohol. This ensures that a wider range of beneficial compounds, including both water-soluble and alcohol-soluble bioactives, are captured. Additionally, alcohol helps partially break down chitin, the tough component in mushroom cell walls, allowing for better extraction of compounds within.
While dual extraction offers a broader spectrum of compounds, it is not always necessary or superior. Hot water extraction is often preferred for certain mushrooms, like Cordyceps, Lion's Mane, Turkey Tail, shiitake, and maitake, as their key active ingredient, beta-glucans, are water-soluble. On the other hand, mushrooms with thicker cell walls, like Reishi and Chaga, benefit from dual extraction due to their mix of water-soluble and alcohol-soluble compounds.
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Hot water extraction is the only clinically validated method for breaking polysaccharides out of cell walls
Hot water extraction is a commonly used method for preparing mushroom supplements. This is because the main active ingredient in most medicinal mushrooms, beta-glucans, is water-soluble. The process involves immersing cells in hot water, causing them to absorb water and expand, leading to a breakdown in the cell wall and facilitating the release of polysaccharides.
Hot water extraction is a simple, convenient, and low-cost method that does not require specialized equipment. However, it is time-consuming and requires high temperatures, which can be a drawback. Additionally, the high temperatures may destroy the structure of polysaccharides, leading to degradation and a reduction in their pharmacological activities.
To overcome the limitations of hot water extraction, alternative methods such as alcohol extraction and dual extraction have been developed. Alcohol extraction, typically using ethanol, is employed to extract alcohol-soluble compounds like triterpenoids, sterols, and flavonoids. On the other hand, dual extraction combines both hot water and alcohol extraction to target a wider range of beneficial compounds, resulting in a more potent and comprehensive supplement.
While dual extraction offers a broader spectrum of benefits, hot water extraction remains the only clinically validated method for breaking polysaccharides out of cell walls. This traditional technique has been used for centuries, even in ancient Traditional Chinese Medicine (TCM) Cordyceps tonics. Modern extractions build upon this foundation, utilizing processes similar to brewing tea to create mushroom supplements with high beta-glucan content for immune support.
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Frequently asked questions
Mushroom extraction is a process that makes the natural, beneficial compounds in mushrooms more readily available to our bodies.
Hot water extraction is a traditional mushroom extraction process that uses hot water to extract water-soluble compounds from mushrooms.
Hot water extraction is used for mushroom supplements because the main active ingredient in most medicinal mushrooms, beta-glucans, are water-soluble.
Hot water extraction is a clinically validated method for breaking down the cell walls of mushrooms and releasing beneficial compounds. It is also a simple and effective process that has been used in traditional Chinese medicine.
Hot water extraction cannot extract all the beneficial compounds from mushrooms, as some compounds are alcohol-soluble. Dual extraction, which uses both hot water and alcohol, may be more effective in some cases.

























