
The number of syringes one should order for mushrooms depends on the type of mushrooms being cultivated, the volume of the syringes, and the desired yield. Syringes for mushroom cultivation typically range from 10 to 60 ml in volume, with 10 ml being a common option. Some sources recommend using 1 ml per jar of substrate, while others suggest that a single 10 ml syringe can inoculate up to 10 jars. Additionally, the yield from each syringe can vary depending on the growth conditions and technique. When ordering syringes for mushrooms, it is essential to consider the specific requirements of the mushroom variety and the desired scale of cultivation.
| Characteristics | Values |
|---|---|
| Syringe size | 10-12 ml, 60 ml, 18 guage 1.5'', 12cc, 10cc, 1cc |
| Number of syringes | 2, 4, 5-pack |
| Syringe contents | Myc water, spore solutions, sporeprints, liquid culture, grain spawn |
| Syringe temperature | Should not freeze, should not exceed 110°F for 2+ hours |
| Syringe storage | Store in the fridge, away from light, for up to 3 months |
| Syringe reuse | Possible, but needle should be sterilized with a lighter |
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What You'll Learn

Syringe size: 10-12ml for spores, 60ml for mycelium water
Syringe size is an important consideration when ordering for mushroom spores and mycelium water. For spores, a 10-12ml syringe is recommended. This size is easy to handle and store, and holds more than enough spores for your needs. Some sources suggest that even a 10ml syringe may be too large, as 1cc is generally plenty per jar of the substrate. Therefore, a 10-12ml syringe will provide more than enough volume for inoculating multiple jars.
For mycelium water, a 60ml syringe is ideal. Smaller syringes are not recommended for mycelium water, as they can be inefficient and inconvenient. A 60ml syringe will allow you to store and handle a larger volume of mycelium water, which is beneficial as mycelium water is handy to have around for various purposes.
It is worth noting that the number of syringes you need will depend on the scale of your operation and your level of experience. Some sources suggest that a single 10ml syringe can be enough to inoculate 10 jars, while others recommend using 2 syringes for the same number of jars. Additionally, the concentration of your spore solution is a factor to consider. It should not be too diluted, but it should also not be packed full of spores.
When ordering syringes, it is important to prioritise quality and sterility. Syringes should be stored properly, with some sources recommending room temperature storage and others suggesting refrigeration. Proper storage will help maintain sterility and increase the lifespan of the syringes, which can be up to several years if stored correctly.
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Syringe quantity: 2-4 recommended for beginners
If you are a beginner, it is recommended that you order 2-4 syringes to start with. This is because syringe plungers can be "sticky", and it can be tricky to avoid injecting too much. With 2-4 syringes, you can get a feel for how much to inject, and you will have a few attempts without needing to reorder.
Syringes typically come in packs of 5 or 10, and each syringe is usually 10ml, although some are 12ml or 60ml. One user on a forum noted that they ordered 4 syringes for $48 and managed to grow over 100 grams of mushrooms. Another user on the same forum noted that they inoculated 18 half-pint jars with one syringe, but that 32 jars was possible, although this would take some practice.
Syringes can be stored unopened in the fridge for up to 3 months, and they will last up to 2-5 years in the fridge. However, it is always recommended to use them as soon as possible, and if you plan to use them within 3-4 months, it is best to store them at room temperature, away from light.
It is important to note that the number of syringes you need depends on your specific project and level of expertise. Some users note that one syringe is enough for them, while others recommend having a few extra in case of mistakes. It is always better to have more than you need than to run out halfway through a project.
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Storage: 2-5+ years in the fridge
Syringes containing liquid culture are best stored in the fridge at 2–4 °C. They can be stored unopened in the fridge for up to 3 months. However, it is important to note that liquid culture syringes containing Pink Oyster mycelium should not be stored in the fridge.
If you have used only a portion of the liquid culture in the syringe, you can store the leftover liquid culture in the fridge for up to 2 weeks. Before using the leftover liquid culture, it is recommended to scrub down the whole syringe with rubbing alcohol and sterilize the needle with a lighter.
Syringes should not be frozen and should not be exposed to temperatures exceeding 110 °F for more than 2 hours. They are typically good for 6-12+ months at room temperature (away from sunlight) and can be stored in the fridge for 2-5+ years. It is always recommended to use them as soon as possible. If you plan to use the syringes within 3-4 months, it is better to store them at room temperature away from light.
To ensure the longevity of the syringes, it is important to follow proper handling and storage procedures, including maintaining optimal temperatures and avoiding contamination.
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Shipping: Cold-resistant and heat-resistant options available
When ordering syringes for mushrooms, it is important to consider the shipping options available to ensure their safe arrival. Syringes containing mushroom spores should not be frozen and must be protected from extreme temperatures during transport. While most of the time there are no issues, it is recommended to opt for cold-resistant and heat-resistant shipping options to safeguard your purchase.
Mushroom spore syringes are typically stored at room temperature, away from direct sunlight, and can last for 6-12 months or longer. However, if you plan to use them after this period, it is best to store them in the fridge, being mindful of the freeze risk. Proper storage ensures that the spores remain viable and effective for their intended purpose.
Some suppliers, like Mushroom Man Mycology, recognize the importance of safe shipping and offer cold-resistant and heat-resistant options for their customers. This ensures that the syringes remain within the recommended temperature range and reduces the risk of temperature-related issues. It is a valuable service for those ordering mushroom syringes, especially in regions with extreme climates.
When choosing a supplier, it is essential to inquire about their shipping methods and temperature control measures. Opt for suppliers who prioritize the safe delivery of their products and offer solutions tailored to your region's climate. This proactive approach ensures that your order arrives intact and that you can confidently proceed with your mushroom cultivation or research plans.
Additionally, consider the quantity of syringes you require. Some suppliers offer discounts on bulk orders, so purchasing multiple syringes can be cost-effective. By planning your mushroom project, you can estimate the number of syringes needed and take advantage of any available discounts, making your endeavor more affordable and efficient.
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Reuse: Possible with proper sterilisation
Syringes are an important tool for cultivating mushrooms. They can be used to inject spores into a growing medium, such as rye grains, sawdust, hay, or cardboard. When using syringes for mushroom cultivation, it is important to maintain proper sterilisation to prevent contamination.
While some sources suggest that certain syringes do not need to be sterilised before use, such as those from www.researchsupply.net, it is generally recommended to sterilise syringes before reusing them. This is because bacteria can grow on the syringe, even when stored in the fridge. To reuse a syringe, it is recommended to scrub down the entire syringe with rubbing alcohol and heat the needle with a lighter until it glows red to sterilise it.
Additionally, it is important to maintain good hand hygiene when handling syringes. Hands should be disinfected with rubbing alcohol before handling the syringe and needle. The syringe should also be shaken or gently flicked to mix the mycelium with the liquid inside. If the syringe is not used all at once, it is important to put the protective cap back on the needle and secure it with parafilm to prevent contamination.
Syringes can be stored for different lengths of time depending on the storage conditions. At room temperature, syringes can be stored for 6-12+ months, while in the fridge, they can last for 2-5+ years. However, it is important to prevent the syringes from freezing and to avoid exposing them to extreme temperatures during shipping.
By following these proper sterilisation and storage techniques, it is possible to reuse syringes for mushroom cultivation safely and effectively.
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Frequently asked questions
The number of syringes you should order depends on the quantity of mushrooms you want to grow. One user on a forum said they inoculated 18 half-pint jars with a single syringe, while another said they inoculated 10 jars with one syringe. A third user said that they regularly inoculate about 32 jars with a single syringe. It is also important to note that syringes should not freeze and should not exceed 110°F for 2+ hours during shipping.
Syringes should be stored at room temperature and away from direct sunlight. They will last 6-12 months when stored this way. If stored in the fridge, they can last up to 2-5 years.
Put the protection cap back on the needle and secure it with parafilm. Store the leftover liquid culture in the fridge and use it within 2 weeks. Before using the liquid culture again, scrub down the syringe with rubbing alcohol and sterilize the needle with a lighter.
Syringes ranging from 10-12ml are preferred for spores as they are easy to handle and store. 60ml syringes are also handy to have around, but they can be a pain to work with.
Yes, Eden Shrooms offers a beginner 5-pack of Psilocybe cubensis spore syringes containing strains that are easy to work with. These kits can help you avoid common pitfalls that beginners encounter and increase your chances of success.

























