
Inoculating mushroom logs is a process that involves transferring mushroom mycelium from one growth medium to another. This method of mushroom cultivation mimics how mushrooms thrive in the wild. The best time of year to inoculate logs is in the late winter, when logs have higher concentrations of sugar. The logs should be cut from healthy, living trees without signs of rot or decay, with a diameter of 4 to 10 inches and a length of 2 to 5 feet. After drilling holes in the logs, spawn is inserted into the holes using an inoculator tool. The logs are then placed in a shady area out of the wind, and the first fruiting can be initiated 9 to 12 months after inoculation.
| Characteristics | Values |
|---|---|
| Best time of year to inoculate | Late winter, when logs have higher sugar concentrations |
| Species to start with | Oyster mushrooms, Shiitake |
| Log health | Fresh, healthy trees with no rot or decay |
| Harvest time | Fall or winter, when sugar concentration is highest |
| Inoculation time after harvest | As soon as possible, no later than a month or two |
| Log diameter | 4-10 inches |
| Log length | 2-5 feet |
| Drill hole depth | 1 1/4 – 1 1/2 inches |
| Hole spacing | 3-4 inches apart within a row, 3-4 inches between rows |
| Number of plugs | Approximately 100 plugs for 3-4 logs |
| Fruiting time | 9-12 months after inoculation |
| Fruiting conditions | Cool temperatures (50-75 degrees Fahrenheit), spring, early summer, or fall |
| Log production | 2.5-4 lbs over its lifetime, lasting up to 5 years or more |
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What You'll Learn
- Choose the right logs: Select fresh, healthy trees with no rot or decay
- Drill holes: Drill holes in a diamond pattern to ensure equal colonisation
- Insert spawn: Use an inoculator to firmly and cleanly insert spawn into the holes
- Wax over: Seal the holes with melted bees or soy wax to protect the inoculation sites
- Initiate fruiting: Shock your logs by submerging them in cool water for 24-48 hours

Choose the right logs: Select fresh, healthy trees with no rot or decay
Choosing the right logs is essential for successfully inoculating mushroom logs. Here are some detailed guidelines to help you select the best logs:
First, it is crucial to select fresh, healthy trees with no rot or decay. Avoid trees that are already decaying, as they may have other species of fungi growing on them, leading to competition for your mushroom spawn. Look for trees without any signs of mushrooms growing on them. Healthy, living trees with high sugar concentrations are ideal for mushroom cultivation. The sapwood and cambium layers of these trees provide essential nutrients for mushrooms, so choose trees with larger sapwood areas to increase productivity.
The best time to harvest trees is during the fall or
Once you've selected the right trees, cut the logs to the appropriate size. Logs should typically be four to six inches in diameter and two to five feet long. If you're using stumps, they should be two to four feet tall. It is best to inoculate the logs as soon as possible after cutting them, ideally within one to two months. The longer you wait, the higher the chance of other mushroom species populating your logs.
After cutting the logs, the next step is to drill holes in the wood using a drill with a specific bit size, such as a 5/16-inch drill bit. Create a diamond pattern of holes to ensure even coverage and an equal chance for the mushroom mycelium to colonize the log. The number of holes will depend on the diameter of the log, ranging from 50 to 250 holes per log.
By following these guidelines for choosing the right logs and preparing them for inoculation, you'll be well on your way to successfully growing mushrooms. Remember, the health and freshness of the logs are critical to giving your mushroom spawn the best environment to thrive.
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Drill holes: Drill holes in a diamond pattern to ensure equal colonisation
Drilling holes in a diamond pattern is a crucial step in inoculating mushroom logs, as it ensures that all parts of the log have an equal opportunity to be colonized by the mushroom mycelium. The number of holes drilled can range from 50 to 250, depending on the diameter of the log. For a four-inch-diameter log, you'll need three rows of holes, with each hole in the row six inches apart. A larger six-inch-diameter log will require nine rows.
When drilling the holes, start two inches from the end of the log and space the holes six to eight inches apart along the length of the log. The holes should be drilled one and a quarter to one and a half inches deep. The specific depth will depend on the type of mushroom spawn you are using. It's important to use the right drill bit size, typically a 5/16-inch bit, to ensure the holes are the correct size for the spawn plugs.
By arranging the holes in a diamond pattern, you maximize the surface area exposed to the mushroom mycelium. This pattern allows for optimal colonization, as the mycelium can spread more effectively along the grain of the wood. The more plugs you use per log, the faster the colonization process will be.
Once you've drilled the holes, the next step is to insert the spawn into them. This is typically done using a tool called an "inoculator," which helps to firmly and cleanly fill the holes with spawn without disturbing the bark of the log. After inoculation, make sure to seal the holes with wax to protect the spawn and maintain moisture levels in the log.
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Insert spawn: Use an inoculator to firmly and cleanly insert spawn into the holes
Once you've drilled the holes, the next step is to insert the spawn. To do this, you'll need an inoculator—a tool that picks up the sawdust spawn and releases it when you depress the end. The goal here is to insert the spawn firmly and cleanly, ensuring that it doesn't stick up above the bark yet completely fills the hole.
For plug spawn, use a 5/16-inch drill bit to create holes that are 1 1/4 to 1 1/2 inches deep. The holes should be spaced about 3 to 4 inches apart within a row, with approximately 3 to 4 inches between the rows. For a 4-inch diameter log, you'll need six rows, a 5-inch log will need seven rows, and a 6-inch log will need nine rows. The number of holes will depend on the diameter of your log, ranging from 50 to 250 holes for a four-foot log.
The holes should be arranged in a diamond pattern to allow the mycelium to spread evenly along the grain of the wood. This pattern ensures that all parts of the log have an equal opportunity to be colonized by the mushroom mycelium. After inoculating each hole, wax over the holes with melted bees or soy wax to protect the spawn.
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Wax over: Seal the holes with melted bees or soy wax to protect the inoculation sites
Waxing over the inoculation sites on mushroom logs is an important step in protecting your mushroom spawn. Beeswax or soy wax can be used to seal the holes, preserving the wood and safeguarding your mushroom spawn from potential invaders.
Beeswax, in particular, has several benefits. Firstly, it provides a natural and chemical-free approach to protecting your mushroom logs. Paint and stain, for instance, may expose your mushroom spawn to strong chemicals, which can be detrimental. Secondly, beeswax is flexible and acts as a binder, adhering well to the wood. Thirdly, it offers protection from environmental factors that can damage the logs, such as rain, sun, and snow, thus preventing rot.
To effectively seal the inoculation sites with wax, follow these steps:
- Melt the wax: Heat the wax to its flash point, ensuring it is as hot as possible without exceeding safety limits.
- Heat the log: Warm the inoculation sites on the log to a temperature similar to that of the melted wax. This helps the wax penetrate the wood effectively.
- Apply the wax: Use a brush or dip the log directly into the melted wax, ensuring the wax covers the inoculation sites and their surrounding areas.
- Allow drying: Give the wax enough time to dry and harden, forming a protective seal over the inoculation sites.
By following these steps, you will successfully seal the inoculation sites with wax, providing your mushroom spawn with a safe and protected environment to grow and thrive.
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Initiate fruiting: Shock your logs by submerging them in cool water for 24-48 hours
Initiating fruiting in mushroom logs requires "shocking" the logs by submerging them in cool water for 24-48 hours. This process mimics the natural monsoon conditions of the native Shiitake habitat. You can use a rain barrel, pond, or bathtub to do this, with a weight to keep them from floating. The logs should be submerged by about 6 inches of water.
After submerging, pat the log dry with paper towels or a clean rag. Then, place the log in your refrigerator overnight. This creates a temperature difference, simulating a cool fall or spring night. This temperature change, along with moisture, kickstarts the fruiting process.
In about a week, mushroom buds will emerge from the log and grow to maturity after another one to two weeks. The best time to fruit your logs is in the spring, early summer, or fall, when daytime temperatures are between 50 and 75 degrees Fahrenheit. During dry weather, mushrooms can harden and stop growing unless they are given a daily sprinkling of water.
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Frequently asked questions
The best time of year to inoculate logs with mushroom plugs is in late winter when logs have higher concentrations of sugar. If you're planting Shiitake, it's recommended to inoculate in early spring if the logs have been cut during the winter.
You should use logs from healthy, living trees without any signs of rot or decay. Oak is particularly good for the cultivation of most mushrooms, but many other species work well, including poplar, aspen, sugar maple, willow, alder and birch. Logs should be four to ten inches in diameter and two to five feet long.
Approximately 100 plugs are needed to inoculate 3-4 logs.
Mushrooms will generally begin producing 6 months to 1 year after inoculation, but this depends on the type of mushroom. Oysters have a shorter incubation period, so if you plant them in late winter, they could start fruiting in the fall of that same year. Shiitake mushrooms have a longer incubation period and may take nine months to two years before they produce mushrooms.
























