Monotubing Mushrooms: A Step-By-Step Guide To Success

how to monotube mushrooms

The monotub method is a popular technique for cultivating mushrooms, offering a simple, cost-effective, and streamlined approach. It involves using a large plastic container with holes for air exchange, filled with a substrate colonized by mycelium. Monotubs are versatile, accommodating various mushroom species, and can be easily scaled up for bulk cultivation. They are particularly effective for growing Psilocybe cubensis, a species that thrives in stable temperatures and humidity monotubs provide. The process is straightforward: prepare the substrate, ensure sterility, maintain optimal temperatures, introduce fresh air and light, and finally, harvest the mushrooms. With their ease of use and impressive yields, monotubs have become a favoured choice for mushroom cultivation.

Characteristics Values
Materials Large Tupperware-style containers, clear or opaque plastic storage containers, wood chips, hardwood-based substrate, micropore tape, monotub filters, Polyfill
Temperature 21-27°C
Lighting Indirect sunlight or room light
Mushroom types Psilocybe Cubensis, edible species of agaricus, shaggy mane, oyster mushrooms, chestnut, namekos
Advantages Cheap, easy to scale up, don't require much equipment or experience, produce large harvests, simple, efficient
Disadvantages Sterility and sterile technique are critical

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Choosing a container: Large, clear or opaque plastic containers with holes for air exchange

When creating a monotub, the container you choose is crucial. Opt for a large, clear or opaque plastic container with holes drilled in it to ensure adequate air exchange. This design allows for the optimal growth of mushrooms by providing the necessary ventilation and airflow.

The size of the container is an important consideration. Large Tupperware-style containers are commonly used for monotubs, as they offer a spacious environment for mushroom growth and can accommodate a substantial amount of substrate. The larger the container, the more mushrooms you can potentially grow.

The material of the container is also key. Plastic is the preferred choice due to its durability, lightweight nature, and affordability. It is easily accessible and can be modified to suit your specific needs. Clear plastic containers offer the advantage of allowing you to observe the growth process and monitor the progress of your mushrooms. However, if you prefer a more discreet option or want to limit light exposure, you can opt for opaque plastic containers.

The holes in the container serve a vital purpose. They facilitate air exchange, allowing fresh air to enter and carbon dioxide to exit. This exchange regulates the oxygen and carbon dioxide levels, creating an optimal environment for mushroom growth. The number, placement, and size of the holes can vary depending on the species of mushrooms you're cultivating, allowing for flexibility in your setup.

To prevent the intrusion of contaminants and competing fungal spores, it's essential to cover the holes with an appropriate material. Polyfill, a polyester stuffing commonly used in pillows and cushions, can be used to fill the holes, acting as a simple and effective microfilter. Alternatively, micropore tape or adhesive monotub filters designed specifically for this purpose can be employed to cover the holes and maintain a sterile environment for your mushroom cultivation.

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Substrate selection: Manure-based for Psilocybe, hardwood for oysters

When it comes to monotub mushroom cultivation, the substrate—the food source that provides the mycelium with nutrients and water—will vary depending on the species being grown. Manure-based substrates are ideal for manure-loving Psilocybe species, such as P. Cubensis, and edible Agaricus species like white button or cremini. This substrate should be sterilized to prevent contamination from competing organisms. A blend of one part coco coir and two parts manure is recommended, with enough water to reach field capacity.

For Psilocybe species, a coconut coir substrate recipe like "Damion5050's Coir Tek" is also an option. This recipe calls for equal parts coco coir and vermiculite by volume, resulting in a loose and airy substrate. Other ingredients that can be added to this substrate include peat moss, brown rice flour, gypsum, agricultural lime, and coffee grounds.

On the other hand, if you're looking to grow oyster mushrooms of the Pleurotus family, a hardwood-based substrate or wood chips are more suitable. Hardwood logs, such as beech, poplar, maple, oak, birch, elm, alder, or cottonwood, are excellent choices for wood-loving mushrooms. These logs should be between 4 and 6 inches in diameter and cut into 4-foot-long pieces. Unlike with manure-based substrates, pasteurization or sterilization is not required for hardwood logs.

To inoculate the logs with mushroom spawn, drill holes about 4 to 6 inches deep, spacing them about 3 inches apart throughout the log. A mix of 50% straw, 30% coffee grounds, 10% grain spawn, and 20% sawdust pellets is another substrate option for growing oyster mushrooms. Additionally, even cardboard makes for an excellent substrate for oyster mushrooms, as it is free, holds moisture well, and facilitates decent air exchange.

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Sterile technique: Critical for success, keep everything clean

Sterility and sterile technique are critical for successfully growing mushrooms. Before you start, familiarise yourself with these concepts and make sure to keep everything clean. For the inoculation phase, find a sanitary environment, or as clean a space as you can. A kitchen surface wiped down with 70% isopropyl alcohol will suffice. Eliminate drafts from your workspace, put on gloves and a face mask, and sanitise your hands, arms, and the area you are working in. Use the isopropyl alcohol spray bottle and a sterile wipe to sanitise the entire monotub lid and base. Make sure the lid to your monotub is also sterilised with rubbing alcohol, and wipe down the sides of the monotub with a paper towel and rubbing alcohol.

When filling and sterilising grain bags, wash and soak the grains to remove dirt and plump them up, ensuring they are ready for sterilisation. Then, fill the bags or jars, leaving some space at the top for expansion. Sterilise at 15 PSI for 90-120 minutes using a pressure cooker or autoclave. Allow the grain bags to cool completely before inoculating with spores or mycelium. Work in a clean, sanitised environment, and wear gloves and a mask to prevent introducing contaminants.

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Temperature control: Maintain 24-27°C for colonisation, then 21-24°C for fruiting

Temperature control is a critical factor in successfully growing mushrooms in a monotub. The optimal temperature range for the colonisation stage is generally agreed to be between 24-27°C. During this stage, the mycelium will grow and colonise the grain. After about 10 days, the monotub should be fully colonised and ready to fruit. However, it is important to note that this process can sometimes take longer, ranging from two to three weeks, depending on factors such as the genetics of the spawn and the incubation temperature.

To initiate the fruiting process, it is necessary to increase oxygen levels by opening the air vents slightly but not fully. Additionally, fresh air and light are essential triggers for mushroom pinning. Therefore, the monotub should be placed in a warm spot that receives indirect sunlight or room light, maintaining a temperature range of 21-24°C. This temperature range is optimal for most mushroom species during the fruiting stage, promoting healthy growth and development.

It is worth mentioning that different mushroom species may have specific temperature preferences, so researching the requirements of your chosen species is essential. While temperatures above 24°C can lead to faster growth, they may also cause issues such as thin and weak stems or caps, reducing the overall yield and quality of the mushrooms. On the other hand, temperatures below 20°C can slow down growth and even lead to dormancy.

To achieve optimal temperature conditions, you can use a heat system such as an incubator specifically designed for mushroom cultivation. Additionally, maintaining sterility and using sterile techniques throughout the process are crucial for successful mushroom growth. By following these temperature guidelines and maintaining a clean environment, you can create favourable conditions for your mushrooms to thrive in your monotub setup.

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Fruiting conditions: Open air vents, provide indirect light to initiate pinning

To create the ideal fruiting conditions for your monotub, you need to understand the role of fresh air and light. These two factors are essential triggers to initiate mushroom pinning. The first step is to open the air vents of your monotub slightly, but not fully, to increase oxygen levels. Think of this as a way to mimic the natural environment where mushrooms thrive—they need a breath of fresh air to begin their growth journey.

The next step is to provide indirect light. Place your monotub in an area that receives indirect sunlight or room light. This light exposure is crucial for triggering the pinning process. Keep in mind that direct sunlight can be harmful, so a gentle, diffused light source is ideal. The temperature range you should aim for during this process is between 21°C and 24°C. This slightly warmer environment, combined with the right amount of light and oxygen, will encourage your mushrooms to enter the fruiting phase.

It's important to note that the specific conditions may vary slightly depending on the species of mushroom you're cultivating. Different mushrooms have unique preferences, so it's always a good idea to research the optimal conditions for your particular variety. However, the principles of providing fresh air and indirect light remain consistent across most species.

Additionally, it's worth mentioning that monotubs are known for their simplicity and ease of use. They don't require intricate equipment or highly specialized knowledge, making them accessible to novice mushroom cultivators. The flexibility of monotubs also allows for experimentation with different substrates and species, so you can explore various options to find what works best for your desired results.

Remember, the key to successful fruiting is creating an environment that mimics the natural habitat of mushrooms. By providing the right balance of fresh air and indirect light, you'll be well on your way to a bountiful mushroom harvest. Happy growing!

Frequently asked questions

A monotub is a single container that provides the perfect microclimate for growing mushrooms. It involves using a large, clear or opaque plastic storage container with holes drilled in it for air exchange.

The monotub method is ideal for beginners as it requires minimal equipment and space. It's also an all-in-one system that can yield impressive results at a low cost.

First, you need to procure mushroom grain spawn. Then, fill the container with a substrate that has been colonized by mycelium. Place the monotub somewhere dark and away from direct sunlight, maintaining a temperature of between 24-27°C. After about 10 days, your monotub should be fully colonized and ready to fruit. To initiate pinning, open the air vents slightly to increase oxygen levels and place the monotub in a warm spot between 21-24°C, where it can receive indirect sunlight or room light. To increase your chances of success, ensure that you maintain sterility and use sterile techniques. You can also automate your monotub by adding accessories like a fan, cycle timer, humidifier, humidity regulator, and LED lighting. Complete monotub kits for beginners are available from local gardening or specialty mycology shops and online retailers. To prevent other fungal spores from entering the chamber, you can cover the holes with Polyfill, micropore tape, or monotub filters.

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