
Mushroom pinning is a crucial stage in the mushroom cultivation process, marking the transition from vegetative growth to the formation of fruiting bodies. It is the process that takes place in the first stages of mushroom formation. When a fungus has enough nutrients, specific environmental conditions will trigger the production of fruiting bodies that we know as mushrooms. To get started with pinning mushrooms, beginners can use a mushroom growing kit, thermometer, and spray bottle. It is important to understand the ideal environmental conditions for the type of mushroom being grown, such as temperature, humidity, and light exposure. Once the substrate is ready, misting can be initiated to increase humidity and trigger pinning. Maintaining optimal conditions and providing a stable growing environment are key to successful pinning and fruiting.
| Characteristics | Values |
|---|---|
| Definition | Mushroom pinning is the process that takes place in the first stages of mushroom formation. |
| Visuals | Tiny bumps on the surface of the mycelium covering the mushroom substrate. |
| Conditions | Optimal conditions include high humidity, fresh air exchange, and specific lighting. |
| Temperature | Lower temperatures of around 53-64°F (12-18°C) are ideal. |
| Humidity | Generally, RH levels of 85% or higher are preferred. |
| Air Exchange | Aim for 3-5 air exchanges per hour to maintain ideal CO2 levels. |
| Lighting | A 12-hour on/off cycle with white-blue spectrum light is optimal. |
| Timeframe | The pinning process can take anywhere from a few days to a couple of weeks. |
| Techniques | Misting the substrate, using a mushroom fruiting chamber, and maintaining proper humidity are crucial. |
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What You'll Learn

Mushroom pinning requirements vary by species
Mushroom pinning requirements do indeed vary by species. For example, shiitake mushrooms require a drop in temperature to initiate pinning, whereas oyster mushrooms prefer higher humidity and more frequent air exchanges. Shiitake mushrooms also benefit from a cold shock – a sudden drop in temperature – to stimulate pinning, while oyster mushrooms benefit from regular misting and increased air circulation.
Some mushroom species are considered photosensitive and require light to form pins. For example, Psilocybe Cubensis and Panaeolus are photosensitive species. In contrast, light is not necessary for the induction of fruiting in fungi, but some spectra have been found to stimulate the initiation of pins and are essential for the normal development of the fruiting body.
The environmental conditions required for pinning also depend on the climate and season. In temperate and continental climates, most mushrooms fruit during the cool, rainy seasons of spring and autumn. In tropical and subtropical climates, mushrooms fruit during the rainy season, and Psilocybe Cubensis and Panaeolus are particularly well-suited to these conditions.
To optimise pinning conditions, it is important to research the specific needs of the species you are cultivating. Understanding and mastering mycelium pinning is essential for anyone interested in growing mushrooms successfully. Maintaining optimal environmental conditions, fine-tuning growing techniques, and learning from the experiences of other growers can help maximise the yield and quality of your harvest.
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Optimal environmental factors for pinning
The following are some optimal environmental factors for pinning mushrooms:
Temperature
The ideal temperature range for most mushrooms is between 53 to 64°F (12 to 18°C). However, certain species, like shiitake mushrooms, require a drop in temperature to initiate pinning. Monitor temperature fluctuations, as these can impact the colonisation process.
Humidity
Mushrooms require high humidity levels of 85% or higher during the pinning process. If the humidity is too low, the pins will dry out and stop growing. To maintain high humidity, mist the substrate regularly with a spray bottle or nozzle attachment. This should be done two to three times a day for the first three to four days. Covering the pallets with a low tunnel made of plastic or metal hoops and greenhouse plastic can also help increase humidity.
Air Exchange
Fresh air exchange is crucial, and stuffy conditions should be avoided. CO2 levels should be kept below 1000-1200 ppm, as higher levels can negatively impact pin set development.
Light
Complete darkness is preferable when the mycelium is actively colonising the substrate, as light can trigger premature pinning. However, during the pinning stage, light becomes an important environmental trigger, signalling the mycelium to transition from vegetative growth to reproductive growth. Sufficient lighting to read comfortably is recommended.
Nutrients
Ensure that the substrate has been fully colonised and provides enough nutrients. If the substrate appears depleted, consider adding supplemental nutrients or transferring the mycelium to a new substrate.
Other Factors
- Research the specific requirements of the mushroom species you are cultivating, as different species have varying pinning preferences. For example, oyster mushrooms prefer higher humidity and more frequent air exchanges.
- Visual confirmation is the best way to determine when the substrate is ready for fruiting conditions. Look for the formation of hyphal knots and primordia.
- Continuously monitor and adjust fruiting conditions, tracking changes in temperature, humidity, and air exchange to maintain an ideal environment.
- Embrace experimentation and trial and error to optimise your growing process and gain a deeper understanding of your mushrooms' needs.
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Signs that your mushrooms are ready for pinning
Mushroom pinning is the process that takes place in the first stages of mushroom formation. It occurs when a fungus has enough nutrients, and specific environmental conditions trigger the production of spore-producing fruiting bodies. Here are some signs that your mushrooms are ready for pinning:
Visual Confirmation of Hyphal Knots and Primordia
Before initiating pinning, it is crucial to visually confirm the presence of hyphal knots and primordia on the substrate. These are tiny bumps or knots that form when the mycelium clumps together. Be patient and wait for these structures to develop before providing fresh air and simulating autumn-like conditions.
Complete Colonization of the Substrate
Ensure that the mycelium has completely colonized the substrate before initiating pinning. The mycelium should cover the substrate densely and may appear as white blobs or bumps. Inadequate colonization may lead to stalled pin growth or increased chances of contamination.
Optimal Environmental Conditions
Monitor and adjust the environmental conditions, including temperature, humidity, and air exchange. For example, oyster mushrooms prefer higher humidity and more frequent air exchanges, while shiitake mushrooms require a drop in temperature to initiate pinning. Research the specific needs of the mushroom species you are cultivating.
Light Exposure
Light serves as a key environmental trigger during the pinning stage. While complete darkness is preferable during the colonization phase, light becomes important to signal the mycelium to transition from vegetative growth to reproductive growth (pinning). Provide sufficient lighting, but avoid excessive direct light, as it can increase substrate temperature and reduce humidity, potentially affecting pin development.
Remember that the specific conditions required for successful pinning may vary depending on the mushroom species and your growing method. Always refer to reliable sources and seek guidance from experienced mushroom growers if needed.
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How to create the right conditions for pinning
Pinning is the process that takes place in the first stages of mushroom formation. It occurs when a fungus has enough nutrients, and specific environmental conditions trigger the production of fruiting bodies. To create the right conditions for pinning, follow these steps:
Firstly, ensure your mycelium has completely colonized the substrate. You will know this has happened when you see the substrate begin to get denser and form white blobs or bumps, which are called hyphal knots. These hyphal knots will continue to grow and develop into tiny matchstick-like structures called primordia or mushroom pins. During this stage, the mycelium requires complete darkness to prevent premature pinning and reduce contamination risks.
Once you observe the formation of these hyphal knots and primordia, it is time to introduce fresh air and autumn-like conditions. This includes maintaining a temperature range of 53 to 64°F (12 to 18°C) and high humidity levels of 85% Relative Humidity (RH) or higher. Aim for 3-5 air exchanges per hour to maintain ideal CO2 levels, as high CO2 levels can inhibit pin formation.
During the pinning stage, it is crucial to maintain relative humidity levels of 90-95% RH to prevent the pins from drying out. However, be cautious not to exceed this range, as higher humidity can lead to mould and bacterial contamination.
Finally, introduce light during the pinning stage. The optimal lighting cycle for pinhead initiation is a 12-hour on/off cycle with white-blue spectrum light (~435-480 nm) or fluorescent light with 6500k lux power.
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Troubleshooting pinning problems
Mushroom pinning refers to the process that takes place in the first stages of mushroom formation. When a fungus has enough nutrients, specific environmental conditions will trigger the production of fruiting bodies that we know as mushrooms. However, there are several issues that can arise during this process.
One common issue is side pinning, which occurs when mushrooms fruit along the sides of their container. This can lead to an uneven and less productive yield, and it can also make harvesting more difficult. Side pinning can be caused by low humidity, a dry substrate surface, mycelial overlay or overgrowth, poor air exchange, or light exposure on the container walls. To prevent side pinning, it is important to maintain optimal fruiting conditions, including proper moisture levels, indirect light exposure, and adequate air exchange. Regularly misting the substrate and creating a humidity dome can help to maintain the necessary high humidity levels. Additionally, techniques such as the fork technique can be used to break up thick mycelium and induce top fruiting.
Another issue that can arise is mushroom pins stalling or failing to grow. This is often due to a lack of humidity, as mushroom pins need high humidity levels of between 85 to 95% while forming. If they dry out, they will stop growing. Excessive moisture can also lead to contamination and other issues, so it is important to find the right balance. Environmental factors, contamination, and bad luck can also contribute to pinning problems and mushroom failure.
If you are experiencing pinning problems, it is important to research the optimal pinning and fruiting conditions for the specific type of mushroom you are trying to grow and replicate those conditions as closely as possible. Additionally, time is often a crucial factor, as some mushroom varieties can take a year or more to produce. Patience and careful observation are key to successful mushroom growth.
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Frequently asked questions
Mushroom pinning is the process that takes place in the first stages of mushroom formation. When a fungus has enough nutrients, specific environmental conditions will trigger the production of fruiting bodies that we know as mushrooms.
Different mushroom species have varying pinning requirements. For example, oyster mushrooms prefer higher humidity and more frequent air exchanges, while shiitake mushrooms require a drop in temperature to initiate pinning. Other conditions include lower temperatures, high humidity, fresh air exchange, and CO2 levels less than 1000ppm.
The time it takes for mushrooms to pin varies depending on the species and environmental conditions. Generally, it can take anywhere from a few days to a couple of weeks for the pins to develop into mature mushrooms. Once pins have formed, the duration from pinning to fruiting typically ranges from 5 to 12 days.

























