
Mushrooms are a nutritious and sustainable food source with a small environmental footprint. They require careful management of carbon dioxide (CO2) levels during cultivation to ensure successful growth and high yields. CO2 levels that are too high can cause issues such as legging, where mushroom pins develop long, thin stems as they reach for oxygen. On the other hand, low oxygen environments can slow spoilage, a preservation method that has been utilised for centuries. Mushrooms also require adequate ventilation to prevent a build-up of CO2 and water vapour, which can lead to suffocation. In addition to CO2 and humidity control, mushrooms need the right combination of temperature and moisture to flower and thrive.
| Characteristics | Values |
|---|---|
| Gases Mushrooms Need | Oxygen, Carbon Dioxide |
| Gases Mushrooms Produce | Carbon Dioxide, Water Vapor |
| Carbon Dioxide Levels During Spawn Run | 10,000 - 20,000 ppm |
| Carbon Dioxide Levels During Fruiting Phase | No more than 1,000 ppm, preferably 500 - 800 ppm |
| Carbon Dioxide Levels During Pinning | 1,200 - 1,500 ppm |
| Carbon Dioxide Emissions for One Pound of Button Mushrooms | 0.7 pounds of CO2 |
| Carbon Dioxide Emissions per Pound of Food Consumed | 0.5 kg of CO2 |
| Carbon Dioxide Emissions per Serving | 0.08 kg of CO2 |
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What You'll Learn

Mushrooms need oxygen to live
Mushrooms are fungi, and like all fungi, they are aerobic organisms. This means that they require oxygen to survive. Mushrooms absorb oxygen from the air and release carbon dioxide as part of their respiration process. They also release water vapour, which is why high humidity is crucial for their growth.
While mushrooms do not photosynthesise and therefore do not require carbon dioxide for growth, carbon dioxide levels are critical in mushroom farming. During the pinning phase, a critical part of the mushroom's growth cycle, carbon dioxide levels that are too high can result in "legging" or long stems as the developing fruit pushes higher in the air to search for oxygen. Conversely, carbon dioxide levels that are too low can result in loose, underdeveloped clusters and fewer mushrooms. Therefore, carbon dioxide levels must be carefully monitored and controlled during mushroom growth to obtain high-quality, large sizes, and overall yield.
In terms of packaging and shelf life, mushrooms can be preserved in a low-oxygen environment, as this slows down spoilage. This preservation method has been used for centuries, as evidenced by ancient Chinese documents that describe the sealing of litchi fruits in clay pots with leaves and grasses before transport. However, dangerous microorganisms, such as Clostridium botulinum, can also flourish in low-oxygen environments, so this preservation method must be carefully managed.
Overall, while mushrooms do require oxygen to live, the role of gases in mushroom growth and preservation is complex and involves careful management of oxygen, carbon dioxide, and water vapour levels.
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Carbon dioxide is important during the pinning stage of growth
Mushrooms absorb oxygen from the air and release carbon dioxide as part of their respiration process. While carbon dioxide is not required for photosynthesis, it is critical for the growth and development of mushrooms.
During the pinning stage, carbon dioxide levels play a crucial role in the growth cycle of mushrooms. This stage marks the formation of baby mushrooms, which later develop into mature fruiting bodies. Maintaining optimal carbon dioxide levels during pinning ensures the development of strong and healthy mushrooms.
The recommended carbon dioxide concentration for the pinning stage varies depending on the mushroom strain. It is generally advised to maintain levels between 1,200 and 1,500 ppm during the early pinning stage for most mushroom varieties. However, it is crucial to gradually lower the carbon dioxide levels once strong pin formation is observed.
Carbon dioxide meters are essential tools for mushroom growers, enabling them to monitor and adjust carbon dioxide levels in the growing environment. These devices help prevent issues such as "legging," where high carbon dioxide levels result in long, thin stems as the mushrooms reach upward in search of oxygen. By maintaining optimal carbon dioxide levels, growers can promote the development of robust mushrooms with desirable characteristics.
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High CO2 levels can cause misshapen mushrooms
Mushrooms are aerobic organisms, meaning they require oxygen to live. They absorb oxygen from the air and release carbon dioxide as part of their respiration process. While carbon dioxide (CO2) is an essential gas for mushroom growth, it must be maintained at the right level to achieve optimal growth and yield.
Oyster mushrooms grown in high-CO₂ environments often develop stretched stems and misshapen caps. This occurs when CO₂ builds up and forms a layer over the mushrooms, blocking access to fresh air. When this happens, mushrooms can’t breathe well and start to suffocate.
To prevent high CO2 levels, mushroom farmers use CO2 meters to monitor and control CO2 levels in their mushroom-growing environment. CO2 sensors can be connected to automated systems that regulate ventilation based on CO2 concentrations, ensuring optimal conditions for mushroom growth.
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Mushrooms require ventilation to avoid suffocation
While mushrooms do not photosynthesize and therefore do not require carbon dioxide for growth, excessive carbon dioxide can deform the fruiting body. For example, oyster mushrooms grown in high-carbon dioxide environments often develop stretched stems and misshapen caps. This phenomenon is known as "legging," where the developing fruit pushes higher in the air to search for oxygen.
To prevent this, mushroom farmers must carefully monitor and control carbon dioxide levels during the pinning or growth phase. Carbon dioxide levels are critical in mushroom farming, along with darkness and humidity. Most experts recommend carbon dioxide levels between 10,000 and 20,000 ppm during the spawn run and no more than 1,000 ppm during the fruiting phase, preferably between 500 and 800 ppm.
Additionally, proper ventilation is crucial to prevent carbon dioxide and water vapour from forming a layer over the mushrooms, blocking their access to fresh air. By ensuring adequate ventilation, mushroom farmers can maintain optimal oxygen levels and avoid suffocating the mushrooms, promoting healthy growth and development.
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Mushrooms have a low carbon footprint
Secondly, mushrooms require less water to grow than other fresh produce items. It takes around 32 glasses of water to grow, harvest, and process one pound of mushrooms, compared to an average of 50 gallons of water per pound of other fresh produce. By controlling water usage and recycling water, mushroom growers can minimize the amount of water they need and reduce the impact of their farms on the surrounding environment.
Thirdly, mushrooms have a low-energy footprint. It takes just one kilowatt-hour (kwh) of electricity to produce one pound of button mushrooms, which is the same amount of energy it takes to run a coffeemaker for one hour each day.
Finally, mushrooms have low CO2 emissions. They absorb oxygen and release carbon dioxide as part of their respiration process, but they do not photosynthesize, so they do not use carbon dioxide for growth. In fact, too much CO2 can deform the fruiting body, causing stretched stems and misshapen caps. Therefore, it is important to monitor and control CO2 levels when growing mushrooms, to ensure high-quality yields.
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Frequently asked questions
Carbon dioxide (CO2) is an essential gas for mushroom growth, but it must be maintained at the right level to achieve optimal growth and yield. Too much CO2 can cause mushrooms to grow too quickly, resulting in poor quality and low yield. On the other hand, too little CO2 can slow down mushroom growth and result in small or deformed mushrooms.
Most experts suggest CO2 levels between 10,000 and 20,000 ppm during the spawn run but no more than 1,000 ppm during the fruiting phase, preferably between 500 and 800 ppm. If the CO2 concentration is higher than 1,000 ppm during fruiting, yields will decrease. Before primordia (pins) appear, the CO2 level shouldn't drop below 2000 ppm.
Mushrooms absorb oxygen from the air and release carbon dioxide as part of their respiration process. They also release water vapour, which is why high humidity is crucial for their growth.

























