
Carbon dioxide (CO2) is a critical factor in mushroom farming. Mushrooms require specific CO2 levels at different stages of growth, and too much or too little can negatively impact their development. Oyster mushrooms, for instance, release a larger amount of CO2 compared to shiitake mushrooms. However, mushroom farming is still considered highly sustainable due to the ability to recycle by-products and waste from other agricultural sectors, as well as the low water and energy requirements of mushroom cultivation.
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What You'll Learn

Oyster mushrooms emit more CO2 than shiitake mushrooms
Oyster mushrooms emit more carbon dioxide (CO2) than shiitake mushrooms. This is due to the temperature-dependent nature of CO2 emission rates for shiitake and oyster mushroom substrates. Oyster mushroom substrates have been shown to have a significantly higher CO2 emission rate, which is twice as high during the incubation stage and even higher during fructification, compared to shiitake substrates.
The cultivation of mushrooms in controlled environments generates a significant amount of CO2 as a byproduct. This presents opportunities for carbon dioxide enrichment in leafy green production. Oyster mushrooms have a notable CO2 enrichment potential, which is greater than that of shiitake mushrooms.
CO2 levels are critical in mushroom farming, and along with darkness and humidity, they are one of the most important factors in growth success. All of these factors must be tightly controlled during mushroom growth to obtain high-quality, large sizes, and overall yield. Most experts suggest CO2 levels between 10,000 and 20,000 ppm during the spawn run but no more than 1,000 ppm during the fruiting phase, preferably between 500 and 800 ppm. If CO2 concentrations are higher than 1,000 ppm during fruiting, yields will decrease.
Mushroom growers control CO2 levels with sensors and controllers, and they require a ventilation system in which the amount of fresh air is automatically regulated. The age of the oyster mushroom at the time of exposure to CO2 is also important. When pins appear, the carbon dioxide level must be reduced. If it remains high, then the development of mushrooms can take different paths depending on other conditions in the growing room, such as temperature, humidity, and airflow speed.
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CO2 levels must be controlled during mushroom farming
Carbon dioxide (CO2) level control is critical in mushroom farming. Along with darkness and humidity, it is one of the most important factors in achieving growth success. All three factors must be tightly controlled during mushroom growth to obtain high-quality, large sizes and overall yield.
CO2 is essential for mushroom respiration. Mushrooms require oxygen and release CO2 during respiration, like humans. However, unlike plants, mushrooms do not photosynthesize, so they do not rely on sunlight or CO2 for energy production. Nevertheless, the levels of CO2 in a mushroom farm significantly affect how mushrooms develop, especially during their fruiting stage. High CO2 levels during the fruiting stage cause mushrooms to develop abnormally long stems as they stretch out to reach areas with lower CO2 and higher oxygen concentrations. This phenomenon is known as "legging". Mushrooms grown in high CO2 environments may also develop small or misshapen caps, which is undesirable for commercial purposes.
To maintain optimal CO2 levels, mushroom farmers can employ various strategies. One simple method is to increase ventilation by using fans and ducts to circulate air in and out of the grow room. Adequate air circulation can help reduce CO2 levels and prevent stagnant pockets of CO2 from building up. Another strategy is to utilise humidifiers and exhaust fans in harmony to balance CO2 and humidity levels. Exhaust fans are particularly effective in reducing CO2 levels, while humidifiers help maintain the high humidity levels mushrooms require. Additionally, modern mushroom farms can utilise CO2 sensors to monitor CO2 levels in real-time. These sensors can be connected to automated systems that regulate ventilation based on CO2 concentrations, ensuring optimal conditions for mushroom growth.
By controlling CO2 levels, farmers can improve the quality and uniformity of their mushroom yields. Proper CO2 control results in mushrooms with thicker caps, shorter stems, and a more uniform appearance, which are highly valued in the market. Furthermore, maintaining optimal CO2 levels can help prevent financial losses by avoiding poorly formed mushrooms that may be rejected by consumers or fetch lower prices. Therefore, it is essential for mushroom farmers to invest in CO2 monitoring and control equipment to ensure the success and profitability of their farms.
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Mushrooms are one of the most sustainably produced foods in the US
Mushroom growers in the US are known as the "ultimate recyclers" for their ability to convert byproducts and waste from other sectors of agriculture into compost or a medium used to grow mushrooms. The unique growing process of mushrooms is unlike that of any other produce item. They start out as mycelium, a branching, thread-like colony of fungus, which is grown in compost. The result is a mushroom. Mushroom farming is the attempt to recreate the natural process of mycelium flowering on a commercial scale.
The production of a pound of mushrooms requires only 1.8 gallons of water and 1.0 kilowatt-hours of energy, and generates only 0.7 pounds of CO2 equivalent emissions. This is a very small carbon footprint when compared to other foods. For example, using one gallon of fuel in the US emits nearly 20 pounds of CO2. Mushrooms are also a low-energy footprint crop, with a serving of mushrooms emitting just 0.08 kg of CO2—only lentils have a lower per-serving CO2 emission level.
CO2 levels are critical in mushroom farming. Along with darkness and humidity, it is one of the most important factors in growth success. All must be tightly controlled to obtain high-quality, large sizes and overall yield. Most experts suggest CO2 levels between 10,000 and 20,000 ppm during the spawn run but no more than 1,000 ppm during the fruiting phase, preferably between 500 and 800 ppm. Oyster mushrooms have been found to release a larger quantity of CO2 compared to shiitake strains.
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Mushrooms are sensitive to CO2 levels during the fruiting stage
Mushrooms are highly sensitive to CO2 levels during the fruiting stage. While CO2 is critical for the development of the fruiting body, excessive CO2 can inhibit the growth of the mycelium and even lead to mushroom poisoning. Therefore, it is important to maintain optimal CO2 levels in the mushroom growing environment.
The ideal CO2 level for mushroom growth is between 10,000 and 20,000 ppm during the spawning process, but it should be reduced to no more than 1,000 ppm during the fruiting phase, with a recommended level between 500 and 800 ppm. This is because mushrooms require higher oxygen levels during the fruiting stage for proper cap development and to prevent stem elongation. If CO2 levels are too high, mushrooms can exhibit abnormal growth, such as elongated stems and underdeveloped caps, reducing their market value.
Different species of mushrooms have specific CO2 requirements during the fruiting stage. For example, Shiitake mushrooms prefer lower CO2 levels (around 600–800 ppm) during the fruiting stage to develop their distinct cap and stem structure, while Button mushrooms have an ideal CO2 concentration range of 800 to 1500 ppm during fruiting.
To ensure optimal CO2 levels, mushroom farmers can employ a variety of techniques and technologies. Proper ventilation is crucial to exchanging CO2-rich air with fresh oxygen during the fruiting stage. Exhaust fans can help reduce CO2 levels, while humidifiers maintain the high humidity levels mushrooms require. CO2 meters are also useful tools that allow farmers to monitor and control CO2 levels in real time, making adjustments as needed to ensure healthy mushroom growth and high yields.
Overall, mushrooms are highly sensitive to CO2 levels during the fruiting stage, and careful management of their growing environment is necessary to achieve successful mushroom farming.
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CO2 levels affect mushroom morphology
Carbon dioxide (CO2) levels are critical in mushroom farming. CO2 levels influence the growth, morphology, and overall yield of mushrooms. Different types of mushrooms have specific environmental needs to flourish. For example, button mushrooms are highly sensitive to CO2 levels and require ideal concentrations between 800 and 1500 ppm during fruiting. If CO2 levels rise beyond this range, button mushrooms develop long, skinny stems and small caps, which are less desirable in the market.
Oyster mushrooms can tolerate slightly higher CO2 levels than button mushrooms, but still require low concentrations (less than 1000 ppm) during fruiting to maintain their proper shape and size. Oyster mushrooms on wood substrate during incubation yielded an average release of 53.5 μg CO2·s⁻1·kgsub⁻1, exhibiting a notable CO2 enrichment potential. Oyster mushrooms have a higher CO2 emission rate than shiitake mushrooms, twice as high during incubation and even higher during fructification.
Shiitake mushrooms prefer lower CO2 levels (around 600–800 ppm) during the fruiting stage to develop their distinct cap and stem structure. Shiitake mushrooms also have a minimal heat exchange rate during incubation, making them an energy-efficient option for cultivation.
CO2 levels play a significant role in the development of mushrooms. During the pinning stage, mushrooms require higher CO2 concentrations (1200-1500 ppm) to initiate growth. However, if CO2 levels remain high as the pins grow, the mushrooms will develop long stems ("legging") as they extend to find oxygen. This results in deformed or stunted mushrooms. Therefore, CO2 levels should be reduced to below 1000 ppm during the fruiting stage, with most experts recommending levels between 500 and 800 ppm.
To optimize mushroom growth and morphology, farmers employ various techniques, such as proper ventilation, CO2 sensors, and other CO2 management practices. By managing CO2 levels effectively, farmers can create an ideal environment for their crops, maximizing yield and ensuring high-quality mushrooms for the market.
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Frequently asked questions
Oyster mushrooms release a larger quantity of CO2 compared to shiitake mushrooms. Oyster mushrooms also release a significant amount of heat during incubation.
The ideal CO2 level for mushroom growth is between 10,000 and 20,000 ppm during the spawning process, but no more than 1,000 ppm during the fruiting phase, preferably between 500 and 800 ppm.
High levels of CO2 can be harmful to mushrooms and may cause them to become discoloured or deformed. It can also inhibit their growth and yield.
A serving of mushrooms emits just 0.08 kg of CO2, which is lower than any other food except lentils.
























