
Spore printing is a simple process that can be done at home with few materials. It is a crucial step in correctly identifying mushrooms, helping to distinguish edible mushrooms from toxic species. Mushroom spores are very small and can only be seen with a microscope. They are the reproductive cells of fungi, comparable to plant seeds. Each fungus produces spores of different shapes and colours, characteristic of the various species. A spore print is the deposit of the spores of a fungus on a surface, usually paper, allowing their colour and density to be visualised. The colour of the spore print is one of the most reliable distinguishing features for differentiating mushroom species.
| Characteristics | Values |
|---|---|
| Purpose | Identification, scientific research, biodiversity study, mushroom cultivation |
| Process | Cut the stem of the mushroom, place the cap on a sheet of paper, cover with a bowl or container, wait for several hours or overnight, observe the spore print |
| Materials | Freshly picked mushroom cap, sheet of white and/or black paper, aluminium foil, glass or container, knife |
| Preservation | Hair spray, artist spray |
| Colour | White, cream, black, red, purple, brown, green, yellow, pale pink, rusty brown, orange |
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What You'll Learn

Spore prints are used to identify mushrooms
Spore prints are a crucial step in correctly identifying mushrooms. They are used to capture and observe the spores of a fungus, helping to identify the fungus in question. Mushroom spores are very small and can only be seen with a microscope. However, by making a spore print, we can see the colour and density of the spores en masse.
Each fungus produces spores of different shapes and colours, characteristic of the various species. The colour of the spore print is one of the most reliable distinguishing features for differentiating mushroom species, especially those that may resemble each other in shape and cap colour. For example, the poisonous false parasol (Chlorophyllum molybdites) has a green spore deposit, while Russula crustosa and Russula virescens have yellowish and white spore deposits, respectively.
To make a spore print, the mushroom cap is carefully separated from the stem, ensuring the gills are not disturbed or squished. The cap is then placed gill-side down on a sheet of paper (usually both black and white paper are used to improve contrast), foil, or another firm surface. A bowl or container is placed over the cap to prevent the spores from being disturbed by the wind or air currents. The mushroom is left for several hours, or even overnight, during which time the spores fall onto the surface and create a spore print pattern.
Spore prints can be preserved by spraying them lightly with an artist spray or hairspray. Spore prints are not only useful for identifying mushrooms but also for studying biodiversity and scientific research, helping to understand the distribution and ecology of different species of fungi.
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They can help distinguish between similar species
Spore prints can be extremely useful in helping to distinguish between similar mushroom species. The colour of the spores is one of the key identifying features of a mushroom and can vary widely, from white to black, with many shades of yellow, brown, purple, pink, and green in between.
While some mushrooms have spores that are a similar colour to the gill, others have surprising colours that are not visible until they are captured on a spore print. For example, the common and widely consumed *
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Prints are made by placing the mushroom on paper
Spore printing is a simple process that can be done at home with few materials. It is a crucial step in correctly identifying mushrooms. Mushroom spores are very small and can only be seen with a microscope. However, by making a spore print, we can see the colour of the spores en masse. Spore colour can range from white to cream, black, or shades of red, purple, or brown.
To make a spore print, you will need a freshly picked mushroom cap, a sheet of paper (white and black paper side by side if you don't know the colour of the spores), a container or a glass bowl to cover the cap, and possibly a sharp knife to prepare the mushroom. First, cut the stem of the mushroom near the base of the cap to expose the gills. Make sure the cap is dry, as moisture can affect the quality of the print. Place the cap on the paper, gill-side down. Cover the cap with a glass bowl or container and leave it for 2 to 24 hours, depending on the humidity and the freshness of the mushroom. The spores will fall on the paper, making a spore print pattern.
If you are collecting mushrooms in the field or woods, you can place the mushroom cap on a sheet of aluminium foil and enclose it, together with the rest of the mushroom, in the foil. You might have a spore print when you return home. If the mushroom is hard, it is more difficult to obtain spore prints. Try wrapping them in wet paper towels or newspapers overnight before putting them down on foil, paper, or glass to make a spore print.
Spore prints can be preserved on paper or foil by spraying them lightly with an artist spray or hair spray. If you want to study the spores with a microscope, scrape off some of the spores from your spore print with a needle or scalpel, and place the spores on a microscope slide. Place a drop of water on the spores and cover them with a cover slip.
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The mushroom's spores fall onto the paper, creating a print
Spore printing is a simple process that can be done at home with a few materials. It is a crucial step in correctly identifying mushrooms. The spores are the reproductive cells of fungi, comparable to plant seeds. Each fungus produces spores of different shapes and colours, characteristic of the various species.
To make a spore print, you will need a freshly picked mushroom cap, a sheet of white and black paper (or an aluminium surface), a container or a glass to cover the cap, and possibly a knife to prepare the mushroom. Cut the stem of the mushroom near the base of the cap to expose the gills. Make sure the cap is dry, as moisture can affect the quality of the print. Place the cap, gill side down, on the paper. Use white and black paper side by side if you don't know the colour of the spore in advance, as some colours may be difficult to see against a light or dark background. Cover the cap with a glass or container to prevent the air from dispersing the spores. Let the mushroom sit for 2 to 24 hours, or overnight. Observe the spore print: after the rest time, carefully remove the cap. You should see a colourful imprint of the spores on the paper. The imprint can show not only colour but also patterns that correspond to the lamellae or pores of the fungus.
The colour of the spore print is one of the most reliable distinguishing features for differentiating mushroom species, especially those that may resemble each other in shape and cap colour. In addition to its usefulness for identification, spore fingerprinting is also valuable for the study of biodiversity and scientific research, helping to understand the distribution and ecology of different species of fungi. For lovers of edible mushrooms, this method helps avoid potentially dangerous errors by distinguishing edible mushrooms from toxic species.
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Prints can be preserved with hair spray
Spore printing is a process used to identify mushrooms. It involves collecting and examining the spores of a mushroom, which are too small to be seen by the naked eye and require a microscope. The colour of the spores can vary, ranging from white to black, and sometimes green. Spore prints are made by placing the mushroom cap on a surface, such as paper, foil, or glass, and allowing the spores to fall and create a print. This process can take several hours, or even a few days, and the resulting prints are delicate and can easily be disturbed or blown away.
To preserve spore prints, one method is to use hair spray. Hair spray can be used to fix the spores onto paper or foil, preventing them from being disturbed or blown away. It is important to hold the hair spray can at least 12 to 15 inches away from the print to avoid blowing the spores off the surface. Additionally, it is recommended to follow the instructions on the hair spray can and to work in a well-ventilated area.
Hair spray is a simple and effective way to preserve spore prints, allowing for the creation of permanent artwork featuring the unique patterns and colours of mushroom spores. This method is accessible to anyone interested in creating spore print art or studying mycology, as it does not require specialised art supplies.
While hair spray is a commonly suggested preservation method, some people may prefer to use alternative fixatives, such as artist spray or Mod Podge, to avoid the strong smell and chemicals associated with hair spray. Additionally, some artists choose to stabilise their spore prints by matting and framing them without the use of any fixative.
Overall, hair spray is a practical and widely available option for preserving spore prints, but it is important to follow safety precautions and consider other preservation methods if desired.
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Frequently asked questions
Spore printing mushrooms outdoors can be done by placing the mushroom cap on a sheet of aluminium foil and enclosing it in your collecting basket. This way, you might have a spore print by the time you return home.
The best surface for a mushroom spore print is a sheet of paper, specifically white and black paper side by side, as some colours may be difficult to see against a light or dark background. However, you can also use other firm surfaces such as aluminium foil, glass, or a black plate.
The amount of time it takes to get a mushroom spore print varies. Some sources say that it takes 2-12 hours or overnight, while others say that it can take up to three days to get a nice spore print.

























