Laura Smith
Psilocybe Cubensis, also known as magic mushrooms, are illegal in many places. It is important to check the laws in your jurisdiction before attempting to cultivate them. If growing Psilocybe Cubensis is legal in your area, you can use a grow kit to cultivate them. The amount of CCs needed for a 3 lb bag of cubensis mushrooms varies depending on the source. Some sources recommend using 2.5-5 CCs per 3 lb bag, while others suggest using 3-5 CCs. One source mentions using 10 CCs or more in a 3 lb bag, but this may be excessive and could result in a higher moisture content in the bag. It is important to note that using too little may result in insufficient mycelium or spores, while using too much can cause the substrate to become too damp, leading to mold and mildew issues.
| Characteristics | Values |
|---|---|
| Amount of CCs in a 3 lb bag | 2.5-5ccs |
| Amount of water to be added to a 3 lb bag | 80 mL |
| Amount of CCs in a 5 lb bag | 10cc or more |
| Amount of water to be added to a 5 lb bag | 100 mL |
| Amount of CCs in a 1 lb bag | 1-3ccs |
| Amount of CCs in a 2 lb bag | 2-4ccs |
| Amount of time to store a 3 lb bag with liquid culture | 1-3 weeks |
| Amount of time to store a 3 lb bag with spores | 2-4 weeks |
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What You'll Learn
2.5-5ccs per 3lb bag
When cultivating cubensis mushrooms, it is essential to follow specific steps to ensure successful growth and avoid contamination. One critical aspect is determining the appropriate amount of liquid culture or spores to inject into the spawn bag. For a 3-pound injection port bag, it is generally recommended to use 2.5 to 5 ccs of liquid. This range ensures that the bag receives sufficient inoculation without adding excessive moisture, which can lead to oversaturation and hinder mycelium colonisation.
It is important to note that the amount of liquid culture or spores required can vary depending on factors such as the specific mushroom strain and the colonisation time desired. Some cultivators suggest using 3 to 5 ccs for a 3-pound bag, while others have successfully used higher amounts, such as 10 ccs or more, in their 3-pound bags. However, it is generally advised not to use an entire syringe in a single 3-pound bag to prevent over-saturation.
To ensure a successful inoculation process, it is crucial to follow sterile techniques and work in a controlled environment. This includes wearing gloves and a mask, cleaning the workstation and bag with alcohol wipes or spray, and using a sterile needle for injection. Additionally, it is recommended to inject the liquid towards the side of the bag rather than the centre, as this allows for faster detection of any contamination.
After inoculation, the colonisation process begins, during which the mycelium grows and spreads throughout the bag. This process can be accelerated by shaking or massaging the bag once it reaches about 25% to 30% colonisation. However, it is important to note that shaking is not recommended when using multispore syringes, as it can disrupt the spores' ability to germinate and meet, forming mycelium. Once the bag is fully colonised, it can be prepared for mushroom growth by transferring it to fruiting conditions or following the specific instructions provided by the mushroom cultivation kit manufacturer.
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3-5ccs for good colonisation
To achieve good colonisation, 3-5 ccs is the recommended amount for a 3-pound bag of cubensis mushrooms. This amount will ensure that the bag is not oversaturated with excessive moisture, which can occur when using larger amounts of liquid culture.
When inoculating the bag, it is important to inject the liquid culture or spores into the centre of the bag, as this will allow for faster colonisation. However, injecting on the side of the bag has its advantages, as it enables you to check for contamination much faster, usually within a few days.
After injecting the liquid culture or spores, it is recommended to let the bag sit undisturbed and out of direct sunlight. Maintaining a temperature between 55 and 75 degrees Fahrenheit is crucial for successful colonisation. Higher temperatures within this range will encourage faster colonisation.
During the colonisation process, it is common to shake or massage the bag to speed up mushroom growth and evenly distribute the mycelium. This step is typically done when the bag is about 20% colonised, and it involves gently kneading and shaking the bag to break up the mycelium until it is evenly distributed throughout.
By following these steps and using 3-5 ccs of liquid culture or spores, you can achieve good colonisation in your 3-pound bag of cubensis mushrooms.
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10ccs for faster colonisation
When growing cubensis mushrooms, it is important to follow the correct procedures for inoculation, colonisation, and fruiting. Sterilisation is critical, as contamination can easily ruin your batch. To achieve faster colonisation, some sources recommend injecting 10ccs of spores or liquid culture into the grow bag. This is higher than the commonly recommended amount of 5ccs, but it is thought to speed up the colonisation process without throwing off the moisture content of the bag.
To begin, you will need to gather your supplies, including gloves, masks, 70% isopropyl alcohol, a spray bottle, a lighter, and syringes. It is important to sterilise your syringe needle before injecting the spores. This can be done by heating the tip of the needle with a lighter and allowing it to cool for about 30 seconds.
Once the needle is sterilised, inject 10ccs of spores or liquid culture into the grow bag. Aim for the bottom and sides of the bag, as this will help with colonisation. If using spores, ensure that all the fluid is injected in a single spot so that the reproductive cells remain close to each other. Keep in mind that higher inoculation rates may improve colonisation times and reduce the risk of contamination.
After injecting the spores or liquid culture, allow the bag to colonise undisturbed in a dark place, with a temperature between 72°F and 80°F. Avoid direct sunlight, and maintain a temperature above 55°F but below 75°F. Keeping the bag at the higher end of this temperature range will encourage faster colonisation. During this colonisation period, the mycelium will start to take over the grain that was inoculated with spores. This process usually takes between three to six weeks, depending on the strain and environmental conditions.
Once your bag reaches about 20% colonisation, you can speed up mushroom growth by gently shaking the bag to distribute the mycelium evenly. This ensures that all the nutrients inside the substrate are consumed. After shaking, place the bag back in storage for another week or two to finish colonising. When the bag is fully colonised, you can begin preparing for mushroom growth by wiping down your workstation, gloves, and bag with alcohol, being careful to avoid the air filter patch.
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1/2 syringe per 3lb bag
Growing Cubensis Mushrooms
Preparing the Work Area and Materials
Before injecting the grow bag, it is important to prepare your work area and materials to prevent contamination. Turn off any fans, air conditioners, or heaters. Put on gloves and a mask, and clean your workstation, gloves, and the outside of the bag with alcohol wipes or 70% isopropyl alcohol. Do not get alcohol inside the air filter patch. Leave a fresh alcohol wipe or paper towel with isopropyl alcohol over the injection port until you are ready to inject.
Injecting the Bag
Attach a sterile needle to your syringe and flame-sterilize it, allowing it to cool before inserting it into the injection port. For a 3-pound bag, inject 2.5 to 5 cc of liquid culture or spores. If using spores, inject all the fluid in a single spot in the bag so the reproductive cells are near each other. Carefully pull out the needle and cover the injection port with tape or an alcohol wipe to prevent contamination.
Storing the Bag
Store the bag in a semi-dark room with no direct sunlight, off the ground, at about 70-78 degrees Fahrenheit (or 55-75 degrees Fahrenheit if incubating). The bag should be stored until it is 20-40% colonized (covered in white mycelium). If using liquid culture, store the bag for about 1-3 weeks, and if using spores, store for 2-4 weeks.
Encouraging Growth
Once the bag is about 20% colonized, gently knead and shake it to break up the mycelium and distribute it evenly throughout the bag. Place the bag back in storage for another week or two until it is fully colonized.
Harvesting Mushrooms
After the bag is fully colonized, wipe down your workstation, gloves, and bag with alcohol, avoiding the air filter patch. Open the bag and harvest the mushrooms by lightly twisting and pulling at the base of the stems. Set the mushrooms aside on a clean surface and rehydrate the growing medium by spraying distilled or RO water down the sides of the bag. For a 3-pound bag, add 80 mL of water, being careful to add it slowly to avoid pooling at the bottom. Close the bag with paper clips and wait two weeks for the next round of mushrooms to grow. After harvesting, cut the bottom portion of each stem and ensure there is no growing medium left on the mushrooms before drying them.
Additional Flushes
You should be able to get 1-2 more flushes of mushrooms out of the bag. Repeat the harvesting and rehydration steps for the second and third flushes. After the third flush, dispose of the grow bag.
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3-5ccs for a 3lb bag of millet
It is important to note that the information provided here is for educational purposes only. The growing of illegal mushrooms, such as Psilocybe Cubensis (magic mushrooms), is not condoned. It is your responsibility to check the laws in your jurisdiction regarding lawful and unlawful mushroom cultivation.
With that said, let's discuss the topic of how many ccs (cubic centimetres) of spore solution are recommended for a 3-pound bag of millet spawn. The general consensus from various sources is that a range of 2.5 to 5 ccs per 3-pound injection port bag is suitable. More specifically, several experienced growers recommend using 3 to 5 ccs for a 3-pound bag of millet. This amount is considered sufficient and allows for more efficient use of the spore syringe, as it can be distributed across multiple bags.
It is worth noting that the amount of spore solution used can depend on various factors, including the type of mushroom being cultivated and personal preferences. Some growers opt for higher amounts, such as 10 to 20 ccs, especially when using liquid culture (LC) or when seeking faster colonisation. However, it is important not to overuse the spore solution, as it can lead to excessive moisture and over-saturation in the bag, creating unfavourable conditions for mycelium growth.
To ensure a successful grow, it is recommended to follow sterile practices when inoculating the grain bag. This includes using clean hands, wearing gloves, and working in a clean environment to minimise the introduction of contaminants. Additionally, it is advisable to wait at least one week after receiving the bag before injecting it, to ensure it was not compromised during transit.
Millet, as a substrate, offers several advantages for mushroom cultivation. It has a lower endospore load compared to rye and wheat, making it less prone to contamination. Its small size provides more inoculation points for the same volume of grain, allowing the mycelium to spread more evenly and abundantly. Millet also encourages rapid mycelial expansion due to its high-nutrient content.
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Frequently asked questions
It is recommended to inject 3 to 5 ccs of spores or liquid culture into a 3 lb bag of cubensis mushrooms.
Yes, shaking the bag is necessary to ensure even distribution of the mycelium and to speed up mushroom growth. However, it is important to wait until the bag is about 20% colonized before shaking.
If using liquid culture, store the bag for about 1-3 weeks. If using spores, store the bag for about 2-4 weeks.
